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Invitrogen
SPY Polyclonal Antibody
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SPY Antibody (PA5-144417) in WB
Western blot analysis of SPY in recombinant protein using a dilution of 1:2,000. Samples were incubated with SPY polyclonal antibody (Product # PA5-144417). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 26.8 kDa. Observed band size: 33 kDa.
产品信息
PA5-144417
产品规格
种属反应
Bacteria
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Escherichia coli (strain K12) SPY protein (52-147aa)
偶联物
形式
Liquid
浓度
3.92 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.03% Proclin 300
保存条件
-20°C or -80°C if preferred
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标信息
An ATP-independent periplasmic chaperone, decreases protein aggregation and helps protein refolding. Binds substrate over a large region of its convex inner surface (PubMed:21317898, PubMed:24497545). Substrate protein folds while it is bound to chaperone (PubMed:26619265). Increasing Spy flexibility increases its substrate affinity and overall chaperone activity (shown for 3 different substrates) (PubMed:24497545). Protects proteins in vitro against tannin inactivation; tannins have antimicrobial activity (PubMed:21317898). Overexpression enhances the stability of otherwise unstable periplasmic proteins (PubMed:21317898).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: b1743; JW1732; Periplasmic chaperone Spyspy
