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SQSTM1 Antibody (701510) in ICC/IF
Immunofluorescence analysis of SQSTM1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SQSTM1 Recombinant Rabbit Monoclonal Antibody (11HC14LC25) (Product # 701510) at 1 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Pl... View More
产品信息
701510
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
11HC14LC25
抗原
proprietary
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532489
产品详细信息
This antibody is predicted to react with bovine, feline, non-human primate, mouse, rat, ovine and porcine based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: EBI3-associated protein of 60 kDa; EBI3-associated protein p60; EBIAP; oxidative stress induced like; phosphotyrosine independent ligand for the Lck SH2 domain p62; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Sequestosome-1; Ubiquitin-binding protein p62
基因别名: A170; FTDALS3; ORCA; OSIL; p60; p62; p62B; PDB3; SQSTM1; ZIP3
UniProt ID: (Human) Q13501
Entrez Gene ID: (Human) 8878
protein serine/threonine kinase activity
protein kinase C binding
protein binding
zinc ion binding
protein kinase binding
receptor tyrosine kinase binding
ubiquitin protein ligase binding
SH2 domain binding
identical protein binding
protein homodimerization activity
ubiquitin binding
K63-linked polyubiquitin binding
mitophagy
positive regulation of protein phosphorylation
immune system process
protein phosphorylation
ubiquitin-dependent protein catabolic process
autophagy
apoptotic process
response to stress
protein localization
regulation of mitochondrion organization
endosomal transport
macroautophagy
positive regulation of macroautophagy
cell differentiation
intracellular signal transduction
positive regulation of apoptotic process
negative regulation of apoptotic process
regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of growth of symbiont in host
positive regulation of transcription from RNA polymerase II promoter
regulation of Ras protein signal transduction
protein heterooligomerization
regulation of protein complex stability
mitophagy in response to mitochondrial depolarization
