Invitrogen

SREBP1 Monoclonal Antibody (2A4)

图: 1 / 4

SREBP1 Antibody (MA5-16124) in WB

Western blot analysis of SREBP1 using a monoclonal antibody (Product # MA5-16124).

Product Image Gallery

产品信息

MA5-16124

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

查看22篇已发表文章 22篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Dog, Chicken, Hamster, Human, Mouse, Rat

已发表种属

Avian, Human, Mouse, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

2A4

抗原

6 His-tag fusion protein of SREBP1 corresponding to amino acids 301-407

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_11152118

产品详细信息

In Western blot, a band can be seen at 125 kDa (precursor) and additional bands may be seen at 60-70 kDa (cleaved).

Suggested positive control: IMR 5 and HeLa cells..

靶标信息

Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2. SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment. In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Ginsenoside F2 Restrains Hepatic Steatosis and Inflammation by Altering the Binding Affinity of Liver X Receptor Coregulators.

Journal of ginseng research

Kim K,Kim MH,Kang JI,Baek JI,Jeon BM,Kim HM,Kim SC,Jeong WI

Published figure using SREBP1 monoclonal antibody (Product # MA5-16124) in Western Blot

Mon Jan 01 00:00:00 UTC 2024

Isthmin-1 is an adipokine that promotes glucose uptake and improves glucose tolerance and hepatic steatosis.

Cell metabolism

Jiang Z,Zhao M,Voilquin L,Jung Y,Aikio MA,Sahai T,Dou FY,Roche AM,Carcamo-Orive I,Knowles JW,Wabitsch M,Appel EA,Maikawa CL,Camporez JP,Shulman GI,Tsai L,Rosen ED,Gardner CD,Spiegelman BM,Svensson KJ

MA5-16124 was used in Western Blotting to identify isthmin-1 (Ism1) as an adipokine and one that has a dual role in increasing adipose glucose uptake while suppressing hepatic lipid synthesis.

Tue Sep 07 00:00:00 UTC 2021

Isthmin-1 is an adipokine that promotes glucose uptake and improves glucose tolerance and hepatic steatosis.

Cell metabolism

Jiang Z,Zhao M,Voilquin L,Jung Y,Aikio MA,Sahai T,Dou FY,Roche AM,Carcamo-Orive I,Knowles JW,Wabitsch M,Appel EA,Maikawa CL,Camporez JP,Shulman GI,Tsai L,Rosen ED,Gardner CD,Spiegelman BM,Svensson KJ

MA5-16124 was used in Western Blotting to identify isthmin-1 (Ism1) as an adipokine and one that has a dual role in increasing adipose glucose uptake while suppressing hepatic lipid synthesis.

Tue Sep 07 00:00:00 UTC 2021

Protein Arginine Methyltransferase PRMT5 Regulates Fatty Acid Metabolism and Lipid Droplet Biogenesis in White Adipose Tissues.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Jia Z,Yue F,Chen X,Narayanan N,Qiu J,Syed SA,Imbalzano AN,Deng M,Yu P,Hu C,Kuang S

MA5-16124 was used in Western Blotting to conclude that PRMT5 plays a critical role in regulating lipid metabolism and lipid droplet biogenesis in adipocytes.

Tue Dec 01 00:00:00 UTC 2020

Protein Arginine Methyltransferase PRMT5 Regulates Fatty Acid Metabolism and Lipid Droplet Biogenesis in White Adipose Tissues.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Jia Z,Yue F,Chen X,Narayanan N,Qiu J,Syed SA,Imbalzano AN,Deng M,Yu P,Hu C,Kuang S

MA5-16124 was used in Western Blotting to conclude that PRMT5 plays a critical role in regulating lipid metabolism and lipid droplet biogenesis in adipocytes.

Tue Dec 01 00:00:00 UTC 2020

TNF-Receptor-1 inhibition reduces liver steatosis, hepatocellular injury and fibrosis in NAFLD mice.

Cell death & disease

Wandrer F,Liebig S,Marhenke S,Vogel A,John K,Manns MP,Teufel A,Itzel T,Longerich T,Maier O,Fischer R,Kontermann RE,Pfizenmaier K,Schulze-Osthoff K,Bantel H

MA5-16124 was used in Western Blot to suggest that selective TNF-receptor-1 inhibition can be used to treat non-alcoholic fatty liver disease by reducing liver fibrosis and injury in mice.

Tue Mar 31 00:00:00 UTC 2020

TNF-Receptor-1 inhibition reduces liver steatosis, hepatocellular injury and fibrosis in NAFLD mice.

Cell death & disease

Wandrer F,Liebig S,Marhenke S,Vogel A,John K,Manns MP,Teufel A,Itzel T,Longerich T,Maier O,Fischer R,Kontermann RE,Pfizenmaier K,Schulze-Osthoff K,Bantel H

MA5-16124 was used in Western Blot to suggest that selective TNF-receptor-1 inhibition can be used to treat non-alcoholic fatty liver disease by reducing liver fibrosis and injury in mice.

Tue Mar 31 00:00:00 UTC 2020

Treg Cells Promote the SREBP1-Dependent Metabolic Fitness of Tumor-Promoting Macrophages via Repression of CD8⁺ T Cell-Derived Interferon-γ.

Immunity

Liu C,Chikina M,Deshpande R,Menk AV,Wang T,Tabib T,Brunazzi EA,Vignali KM,Sun M,Stolz DB,Lafyatis RA,Chen W,Delgoffe GM,Workman CJ,Wendell SG,Vignali DAA

MA5-16124 was used in Flow cytometry/Cell sorting to show that Treg cells shape the transcriptional landscape across multiple tumor-infiltrating immune cell types.

Tue Aug 20 00:00:00 UTC 2019

TTC39B deficiency stabilizes LXR reducing both atherosclerosis and steatohepatitis.

Nature

Hsieh J,Koseki M,Molusky MM,Yakushiji E,Ichi I,Westerterp M,Iqbal J,Chan RB,Abramowicz S,Tascau L,Takiguchi S,Yamashita S,Welch CL,Di Paolo G,Hussain MM,Lefkowitch JH,Rader DJ,Tall AR

MA516124 was used in western blot to describe the ubiquitination and degradation of liver X receptor

Thu Jul 14 00:00:00 UTC 2016

Reprogramming towards anabolism impedes degeneration in a preclinical model of retinitis pigmentosa.

Human molecular genetics

Zhang L,Justus S,Xu Y,Pluchenik T,Hsu CW,Yang J,Duong JK,Lin CS,Jia Y,Bassuk AG,Mahajan VB,Tsang SH

MA516124 was used in western blot to assess a non-gene-specific strategy to reprogram photoreceptors towards anabolism by upregulating the mechanistic target of rapamycin pathway

Sat Oct 01 00:00:00 UTC 2016

PPARδ activation attenuates hepatic steatosis in Ldlr-/- mice by enhanced fat oxidation, reduced lipogenesis, and improved insulin sensitivity.

Journal of lipid research

Bojic LA,Telford DE,Fullerton MD,Ford RJ,Sutherland BG,Edwards JY,Sawyez CG,Gros R,Kemp BE,Steinberg GR,Huff MW

MA5-16124 was used in western blot to study the roles of decreased lipogenesis, increased beta-oxidation and enhanced insulin-sensitivity in the mechanism by which PPAR-delta protects against hepatic steatosis in LDLR-deficient mice

Tue Jul 01 00:00:00 UTC 2014

The epigenetic drug 5-azacytidine interferes with cholesterol and lipid metabolism.

The Journal of biological chemistry

Poirier S,Samami S,Mamarbachi M,Demers A,Chang TY,Vance DE,Hatch GM,Mayer G

MA5-16124 was used in western blot to study the DNA methylation-independent mechanism by which the epigenetic drug 5-azacytidine inhibits the expression of genes involved in cholesterol and lipid metabolism

Fri Jul 04 00:00:00 UTC 2014

Effect of cholesterol on lipogenesis and VLDL-TG assembly and secretion in goose primary hepatocytes.

Molecular and cellular biochemistry

Han CC,Wang JW,Pan ZX,Tang H,Xiang SX,Wang J,Li L,Xu F,Wei SH

MA5-16124 was used in western blot to study lipogenesis and the assembly and secretion of VLDL-triglyceride in goose primary hepatocytes and the effects of different concentrations of cholesterol

Fri Feb 01 00:00:00 UTC 2013

Lack of liver X receptors leads to cell proliferation in a model of mouse dorsal prostate epithelial cell.

PloS one

Dufour J,Pommier A,Alves G,De Boussac H,Lours-Calet C,Volle DH,Lobaccaro JM,Baron S

MA5-16124 was used in western blot to study altered signaling pathways in prostate cells isolated from Lxralphabeta knockout mice

Thu Oct 17 00:00:00 UTC 2013

Glucose 6-phosphate, rather than xylulose 5-phosphate, is required for the activation of ChREBP in response to glucose in the liver.

Journal of hepatology

Dentin R,Tomas-Cobos L,Foufelle F,Leopold J,Girard J,Postic C,Ferré P

MA5-16124 was used in western blot to study the role of glucose-6-phosphate as the glucose-signaling metabolite that activates ChREBP in response to hepatic glucose

Sun Jan 01 00:00:00 UTC 2012

The lipogenic transcription factor ChREBP dissociates hepatic steatosis from insulin resistance in mice and humans.

The Journal of clinical investigation

Benhamed F,Denechaud PD,Lemoine M,Robichon C,Moldes M,Bertrand-Michel J,Ratziu V,Serfaty L,Housset C,Capeau J,Girard J,Guillou H,Postic C

MA5-16124 was used in western blot to study the role of the lipogenic transcription factor ChREBP in hepatic steatosis and insulin resistance in humans and mice

Fri Jun 01 00:00:00 UTC 2012

Evaluation of Fbxw7 expression and its correlation with expression of SREBP-1 in a mouse model of NAFLD.

Molecular medicine reports

Tu K,Zheng X,Yin G,Zan X,Yao Y,Liu Q

MA5-16124 was used in immunohistochemistry to study the relationship between Fbxw7 and SREBP-1 in a murine model of non-alcoholic fatty liver disease

Sat Sep 01 00:00:00 UTC 2012

Inhibition of intestinal bile acid transporter Slc10a2 improves triglyceride metabolism and normalizes elevated plasma glucose levels in mice.

PloS one

Lundåsen T,Andersson EM,Snaith M,Lindmark H,Lundberg J,Östlund-Lindqvist AM,Angelin B,Rudling M

MA5-16124 was used in western blot to study the beneficial changes in triglyceride metabolism and plasma glucose concentrations of ob/ob obese mice following inhibition of intestinal Slc10a2

Thu Dec 13 00:00:00 UTC 2012

The oxysterol 24(s),25-epoxycholesterol attenuates human smooth muscle-derived foam cell formation via reduced low-density lipoprotein uptake and enhanced cholesterol efflux.

Journal of the American Heart Association

Beyea MM,Reaume S,Sawyez CG,Edwards JY,O'Neil C,Hegele RA,Pickering JG,Huff MW

MA5-16124 was used in western blot to study the role of alterations in LDL and cholesterol metabolism in the mechanism by which an oxysterol reduces smooth muscle cell foam cell formation

Fri Jun 01 00:00:00 UTC 2012

Distinct regulation of adiponutrin/PNPLA3 gene expression by the transcription factors ChREBP and SREBP1c in mouse and human hepatocytes.

Journal of hepatology

Dubuquoy C,Robichon C,Lasnier F,Langlois C,Dugail I,Foufelle F,Girard J,Burnol AF,Postic C,Moldes M

MA5-16124 was used in western blot to study the differential roles of ChREBP and SREBP1c in regulating adiponutrin expression in mouse and human hepatocytes

Fri Jul 01 00:00:00 UTC 2011

The TRC8 ubiquitin ligase is sterol regulated and interacts with lipid and protein biosynthetic pathways.

Molecular cancer research : MCR

Lee JP,Brauweiler A,Rudolph M,Hooper JE,Drabkin HA,Gemmill RM

MA5-16124 was used in western blot to study the role of sterol-regulated TRC8 ubiquitin ligase in regulation of lipid and protein biosynthetic pathways

Fri Jan 01 00:00:00 UTC 2010

Regulation of energy substrate utilization and hepatic insulin sensitivity by phosphatidylcholine transfer protein/StarD2.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Scapa EF,Pocai A,Wu MK,Gutierrez-Juarez R,Glenz L,Kanno K,Li H,Biddinger S,Jelicks LA,Rossetti L,Cohen DE

MA5-16124 was used in western blot to examine the role of phosphatidylcholine transfer protein/StarD2 in hepatic insulin sensitivity

Tue Jul 01 00:00:00 UTC 2008

Liver-specific inhibition of ChREBP improves hepatic steatosis and insulin resistance in ob/ob mice.

Diabetes

Dentin R,Benhamed F,Hainault I,Fauveau V,Foufelle F,Dyck JR,Girard J,Postic C

MA5-16124 was used in western blot to study the beneficial effects of shRNA-mediated knockdown of ChREBP on hepatic steatosis and insulin sensitivity in ob/ob leptin-deficient mice

Tue Aug 01 00:00:00 UTC 2006

Insulin regulation of glucokinase gene expression: evidence against a role for sterol regulatory element binding protein 1 in primary hepatocytes.

FEBS letters

Gregori C,Guillet-Deniau I,Girard J,Decaux JF,Pichard AL

MA5-16124 was used in western blot to study the lack of SREBP-1 involvement in the regulation of glucokinase gene expression in primary hepatocytes

Mon Jan 23 00:00:00 UTC 2006

Polyunsaturated fatty acids suppress glycolytic and lipogenic genes through the inhibition of ChREBP nuclear protein translocation.

The Journal of clinical investigation

Dentin R,Benhamed F,Pégorier JP,Foufelle F,Viollet B,Vaulont S,Girard J,Postic C

MA5-16124 was used in western blot to study the mechanism by which polyunsaturated fatty acids suppress the expresson of genes involved in glycolysis and lipogenesis

Sat Oct 01 00:00:00 UTC 2005

Hepatic farnesyl diphosphate synthase expression is suppressed by polyunsaturated fatty acids.

The Biochemical journal

Le Jossic-Corcos C,Gonthier C,Zaghini I,Logette E,Shechter I,Bournot P

MA5-16124 was used in western blot to study the mechanism by which polyunsaturated fatty acids suppress liver farnesyl diphosphate synthase expression

Tue Feb 01 00:00:00 UTC 2005

Hepatic glucokinase is required for the synergistic action of ChREBP and SREBP-1c on glycolytic and lipogenic gene expression.

The Journal of biological chemistry

Dentin R,Pégorier JP,Benhamed F,Foufelle F,Ferré P,Fauveau V,Magnuson MA,Girard J,Postic C

MA5-16124 was used in western blot to study the role of hepatic glucokinase in the synergistic action of ChREBP and SREBP-1c on glycolytic and lipogenic gene expression

Fri May 07 00:00:00 UTC 2004

生物信息学

蛋白别名: ADD1; Adipocyte determination- and differentiation-dependent factor 1; bHLHd1; Class D basic helix-loop-helix protein 1; nt-SREBP1; SREBP-1; SREBP1; sterol regulatory element binding protein 1; sterol regulatory element binding protein-1; sterol regulatory element binding-protein 1; Sterol regulatory element-binding protein 1; Sterol regulatory element-binding transcription factor 1

查看更多收起

基因别名: ADD-1; ADD1; BHLHD1; D630008H06; I79_010155; SREBF1; SREBP-1; SREBP-1a; SREBP-1c; SREBP1; SREBP1a; SREBP1c