Invitrogen

STING Monoclonal Antibody (OTI4H1)

STING Antibody in Immunocytochemistry (ICC/IF)

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STING Antibody (MA5-26030) in ICC/IF

Immunofluorescence of STING1 was performed using PMA-treated THP-1 WT and STING1 KO cells that were labeled with a green or a far-red fluorescent dye, respectively. Post-labeling, WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with an optically clear flat bottom as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min at RT; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS containing 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min at RT. Cell... View More

STING Antibody in Immunohistochemistry (Paraffin) (IHC (P))

STING Antibody in Immunoprecipitation (IP)

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STING Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. THP-1 cells were transfected with STING siRNA and decrease in signal intensity was observed in western blot application using STING Monoclonal Antibody (OTI4H1) (Product # MA5-26030). {KD}

产品信息

MA5-26030

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

OTI4H1

抗原

Full length human recombinant protein of TMEM173 produced in HEK293T cell

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 1% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2725437

靶标信息

STING is a 379 amino acid containing signaling protein that acts as an important component of innate immune signaling. This signaling protein is able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon (IFN-alpha and IFN-beta) and exert a potent anti-viral state following expression. STING may have translocon function, the translocon possibly being able to influence the induction of type I interferons and is also involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II) thus facilitating the detection of intracellular viral RNA species as well as B-form DNA and mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway. Generally seen in endoplasmic reticulum membrane, cell membrane and mitochondrion outer membrane, it is ubiquitously expressed in most of tissues.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Activated cGAS/STING signaling elicits endothelial cell senescence in early diabetic retinopathy.

JCI insight

Liu H,Ghosh S,Vaidya T,Bammidi S,Huang C,Shang P,Nair AP,Chowdhury O,Stepicheva NA,Strizhakova A,Hose S,Mitrousis N,Gadde SG,Mb T,Strassburger P,Widmer G,Lad EM,Fort PE,Sahel JA,Zigler JS,Sethu S,Westenskow PD,Proia AD,Sodhi A,Ghosh A,Feenstra D,Sinha D

MA5-26030 was used in Immunohistochemistry to suggest that targeting STING may be an effective therapy for the early prevention and treatment of DR.

Thu Jun 22 00:00:00 UTC 2023

生物信息学

蛋白别名: endoplasmic reticulum IFN stimulator; Endoplasmic reticulum interferon stimulator; ERIS; hMITA; hSTING; Mediator of IRF3 activation; mitochondrial mediator of IRF3 activation; N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner; Stimulator of interferon genes protein; tm173; Transmembrane protein 173

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基因别名: ERIS; hMITA; hSTING; MITA; MPYS; NET23; SAVI; STING; STING1; TMEM173

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UniProt ID: (Human) Q86WV6

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Entrez Gene ID: (Human) 340061

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功能

protein binding transcription factor binding protein kinase binding ubiquitin protein ligase binding cyclic-di-GMP binding identical protein binding protein homodimerization activity cyclic-GMP-AMP binding

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