Invitrogen

STING Recombinant Rabbit Monoclonal Antibody (PSH07-44)

STING Antibody in Immunocytochemistry (ICC/IF)

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STING Antibody (MA562089) in ICC/IF

Immunofluorescence analysis of STING was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with STING Recombinant Rabbit Monoclonal Antibody (PSH07-44) (Product # MA562089) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790, 1:2,000 dilution) for 45 ... View More

STING Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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STING Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell line and tissue models owing to their inherent genetic constitution. Western blot analysis using STING Recombinant Rabbit Monoclonal Antibody (PSH07-44) (Product # MA562089) shows STING expression in THP-1, K-562, Jurkat and Mouse Spleen which are high expressors of STING and not in Jeko-1, A549, T-47D and Mouse Liver, which are low expressors. {RE}

产品信息

MA562089

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000

免疫组化（石蜡） (IHC (P))

1:4,000

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

1:1,000

免疫沉淀 (IP)

1-2 µg

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

PSH07-44

抗原

Recombinant protein within human STING aa 117-379.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Positive Control: HDLM-2 cell lysate, THP-1 cell lysate, HT-29 cell lysate, HepG2 cell lysate, HaCaT cell lysate, HL-60 cell lysate, HEK-293 cell lysate, K-562 cell lysate, A20 cell lysate, C2C12 cell lysate, EL4 cell lysate, Rat thymus tissue lysate, human lung tissue, human tonsil tissue, THP-1, PC-12.

Tissue Specificity: Low tissue specificity.

Subcellular Location: Endoplasmic reticulum membrane, Cytoplasm, perinuclear region, Endoplasmic reticulum-Golgi intermediate compartment membrane, Golgi apparatus membrane, Cytoplasmic vesicle, autophagosome membrane, Mitochondrion outer membrane, Cell membrane.

Sequence Similarities: 74%Mouse/Rat.

Predicted band size: 42 kDa.

靶标信息

STING is a 379 amino acid containing signaling protein that acts as an important component of innate immune signaling. This signaling protein is able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon (IFN-alpha and IFN-beta) and exert a potent anti-viral state following expression. STING may have translocon function, the translocon possibly being able to influence the induction of type I interferons and is also involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II) thus facilitating the detection of intracellular viral RNA species as well as B-form DNA and mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway. Generally seen in endoplasmic reticulum membrane, cell membrane and mitochondrion outer membrane, it is ubiquitously expressed in most of tissues.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: endoplasmic reticulum IFN stimulator; Endoplasmic reticulum interferon stimulator; ERIS; hMITA; hSTING; Mediator of IRF3 activation; mitochondrial mediator of IRF3 activation; mitochondrial transmembrane protein 173; MMITA; mSTING; N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner; rSTING; Stimulator of interferon genes protein; tm173; Transmembrane protein 173

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基因别名: 2610307O08Rik; ERIS; hMITA; hSTING; MITA; MPYS; NET23; RGD1562552; SAVI; STING; STING1; TMEM173

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UniProt ID: (Human) Q86WV6, (Mouse) Q3TBT3, (Rat) F1M391

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Entrez Gene ID: (Human) 340061, (Mouse) 72512, (Rat) 498840

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功能

protein binding transcription factor binding protein kinase binding ubiquitin protein ligase binding cyclic-di-GMP binding identical protein binding protein homodimerization activity cyclic-GMP-AMP binding nucleotide binding

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