Invitrogen

STMN2 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1个已发表图片

3 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

STMN2 Antibody in Immunocytochemistry (ICC/IF)

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STMN2 Antibody (720178) in ICC/IF

Immunofluorescence was performed on fixed and permeabilized SHSY-5Y cells for detection of Stathmin-2 using Anti-Stathmin-2 Rabbit Polyclonal Antibody (Product # 720178, 1 µg/mL), alpha-Tubulin was detected using Anti-alpha Tubulin Monoclonal Antibody (Product # 32-2500, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000), Goat anti-Mouse IgG Secondary Antibody, Alexa Fluor®594conjugate (Product # A-11032, 1:400) respectively. Panel a) shows repr... View More

Product Image Gallery

STMN2 Antibody — Figure 4 Silencing Stathmin-2 increased glucagon secretion and overexpression of stathmin-2 suppressed glucagon secretion in alphaTC1-6 cells. Wild type (wt; control) and stathmin-2 depleted (Stmn2-KD) aTC1-6 cells were pre-incubated 2 h in serum-free medium and then incubated with or without KCl (55 mM) for 15 min. (A) Glucagon secretion is significantly stimulated by KCL in wt cells, while in Stmn-KD cells, basal glucagon secretion is increased and does not respond to KCl. * p < 0.01 compared to basal secretion in wt cells. (B) Stmn2 mRNA levels are decreased by about 70% after siRNA-mediated depletion in alphaTC1-6 cells. Values are means +- SEM ( n = 5), * p < 0.01. (C) Stathmin-2 protein levels are depleted after siRNA-mediated silencing of Stmn2. C (control); KD (gene silenced). Beta-actin was used as a loading control. (D) Proglucagon mRNA levels are not affected by siRNA-mediated depletion of stathmin-2. (E) Glucagon secretion is inhibited by overexpression of Stmn2. alphaTC1-6 cells were transfected with pcDNA3.1 (+) MAr-stmn2 or empty vector (negative control). Both basal and K + -stimulated glucagon secretion were inhibited by overexpression of Stmn2. Values are means +- SEM ( n = 4). * p < 0.05; ** p < 0.001 compared to unstimulated control. {KD}

产品信息

720178

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-1 µg/mL

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免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human STMN2 (aa 29-179)

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偶联物

Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.1% BSA, 30% glycerol

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2606219

产品详细信息

These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.

靶标信息

STMN2, also known as SCG-10, is a neuronal growth-associated protein that shares significant amino acid sequence similarity with the phosphoprotein stathmin. STMN2 may play a role in neuronal differentiation, and in modulating membrane interaction with the cytoskeleton during neurite outgrowth. Reductions in the expression of this gene have been associated with Down's syndrome and Alzheimer's disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Stathmin-2 Mediates Glucagon Secretion From Pancreatic α-Cells.

Frontiers in endocrinology

Asadi F,Dhanvantari S

720178 was used in Western Blotting to describe a potential new pathway for the regulation of glucagon secretion, and may have implications for controlling glucagon hypersecretion in diabetes.

Wed Feb 03 00:00:00 EST 2021

The landscape of multiscale transcriptomic networks and key regulators in Parkinson's disease.

Nature communications

Wang Q,Zhang Y,Wang M,Song WM,Shen Q,McKenzie A,Choi I,Zhou X,Pan PY,Yue Z,Zhang B

720178 was used in Western Blotting to develop multiscale gene network models of Parkinson's disease and identify potential key regulators of the disease which require further investigation.

Wed Nov 20 00:00:00 EST 2019

The landscape of multiscale transcriptomic networks and key regulators in Parkinson's disease.

Nature communications

Wang Q,Zhang Y,Wang M,Song WM,Shen Q,McKenzie A,Choi I,Zhou X,Pan PY,Yue Z,Zhang B

720178 was used in Western Blotting to develop multiscale gene network models of Parkinson's disease and identify potential key regulators of the disease which require further investigation.

Wed Nov 20 00:00:00 EST 2019

生物信息学

蛋白别名: neuron-specific growth-associated protein; neuronal growth-associated protein (silencer element); Protein SCG10; SGC10; Stathmin-2; stathmin-like 2; superior cervical ganglia, neural specific 10; Superior cervical ganglion-10 protein; superiorcervical ganglia, neural specific 10

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基因别名: AI159727; SCG10; SCGN10; Stmb2; STMN2

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UniProt ID: (Human) Q93045, (Rat) P21818, (Mouse) P55821

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Entrez Gene ID: (Human) 11075, (Rat) 84510, (Mouse) 20257

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功能

protein binding tubulin binding calcium-dependent protein binding

参与通路

negative regulation of microtubule depolymerization positive regulation of neuron projection development negative regulation of neuron projection development negative regulation of microtubule polymerization positive regulation of microtubule depolymerization cellular response to nerve growth factor stimulus microtubule depolymerization neuron projection development regulation of cytoskeleton organization regulation of microtubule polymerization or depolymerization