Abnova

SYP Recombinant Rabbit Monoclonal Antibody

Name: SYP Recombinant Rabbit Monoclonal Antibody
Brand: Abnova
SKU: RAB02543

查看其他44个Synaptophysin抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)

SYP Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 3

SYP Antibody (RAB02543) in ICC/IF

Immunofluorescent analysis of NIH3T3 cells with SYP recombinant monoclonal antibody (Product # RAB02543). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

SYP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

RAB02543

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫组化（石蜡） (IHC (P))

1:50-1:100

免疫细胞化学 (ICC/IF)

1:50-1:100

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

50mM tris glycine, pH 7.4, with 0.05% BSA, 150mM NaCl, 50% glycerol

内含物

0.01% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_3711069

靶标信息

Synaptophysin is a calcium-binding and integral membrane glycoprotein present in presynaptic vesicles in almost all neurons. Synaptophysin has four transmembrane domains and it forms a complex with dynamin at high calcium concentrations suggesting an involvement in synaptic vesicle endocytosis. It is also involved in the regulation of short-term and long-term synaptic plasticity. Synaptophysin is currently the most widely used marker for nerve terminals and for differentiating neuroendocrine tumors. Mutations in the gene can result in mental retardation, X-linked 96.

仅用于科研。不用于诊断过程。未经明确授权不得转售。