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| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
1:100-1:1,000 | 查看1篇已发表文章 1篇已发表文章 |
免疫组化（石蜡） (IHC (P)) |
1:100-1:200 | - |
免疫组化（冰冻） (IHC (F)) |
1:100-1:200 | - |
免疫细胞化学 (ICC/IF) |
1:100-1:200 | 查看1篇已发表文章 1篇已发表文章 |
| 产品规格 | |
|---|---|
种属反应 |
Dog, Chicken, Cat, Human, Mouse, Non-human primate, Pig, Rat |
已发表种属 |
Human, Pig |
宿主/亚型 |
Mouse / IgG1 |
分类 |
Monoclonal |
类型 |
Antibody |
克隆号 |
R4A |
抗原 |
Cytoskeletal smoothelin extract of chicken gizzard. |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
1 mg/mL |
纯化类型 |
purified |
保存液 |
PBS |
内含物 |
0.09% sodium azide |
保存条件 |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2239715 |
OMA1-06020 detects smoothelin from human, canine, feline, porcine, chicken and monkey tissues. This antibody detects both the visceral and vascular smoothelin isoforms. Does not cross react with rat samples.
OMA1-06020 has been successfully used in Western blot, immunocytochemistry, and immunohistochemistry procedures. By Western blot, this antibody detects an ~59 kDa protein representing visceral smoothelin and an ~100 kDa protein representing vascular smoothelin from canine smooth muscle extracts. This antibody can be used to discriminate fully differentiated (contractile) smooth muscle cells from proliferative smooth muscle cells. Staining of formalin-fixed paraffin-embedded tissues requires boiling in 10 mM citrate buffer (pH 6.0) for 3x5 min, prior to immunostaining.
OMA1-06020 antigen is cytoskeletal smoothelin extract of chicken gizzard.
Store at 4°C, or in small aliquots at -20°C.
Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are a useful tool to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses reveal a broad species distribution of this protein. Cells with SMC-like characteristics, such as myofibroblasts, myoepithelial cells, and skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections, as well as transfection studies, show a filamentous organization of smoothelin that is different from that of desmin and vimentin. Smoothelin colocalizes with actin stress fibers. Two tissue specific isoforms have been identified: a 59 kDa isoform specific for visceral SMC; and a 100 kDa specific for vascular SMC. Human smoothelin is encoded by a single copy gene which is located on chromosome 22.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: FLJ35365; FLJ38597; Smoothelin; smoothelin-B; SMTN
基因别名: SMSMO; SMTN
UniProt ID: (Human) P53814, (Mouse) Q921U8
Entrez Gene ID: (Human) 6525, (Chicken) 100858894, (Pig) 414369, (Dog) 477542, (Mouse) 29856, (Rat) 289734
