Invitrogen

Smoothelin Monoclonal Antibody (R4A)

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Smoothelin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry on paraffin section of human smooth muscle stained with Smoothelin monoclonal antibody (Product # OMA1-06020).

Smoothelin Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry on frozen section of chicken gizzard striated muscle stained with Smoothelin monoclonal antibody (Product # OMA1-06020).

Smoothelin Antibody in Western Blot (WB) — Western blot was performed using Anti-Smoothelin Monoclonal Antibody (R4A) (Product # OMA1-06020) and a 100kDa band corresponding to Smoothelin was observed across cell lines and tissues tested . Nuclear enriched extracts (30 µg lysate) of PC-3 (Lane 1), HeLa (Lane 2), Mouse Uterus (Lane 3), Mouse Brain (Lane 4) and Mouse Lung (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:5000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

产品细节

Applications

Tested Dilution

Publications

免疫印迹 (WB)

1:100-1:1,000

View 1 publication 1 publication

免疫组化（石蜡） (IHC (P))

1:100-1:200

免疫组化（冰冻） (IHC (F))

1:100-1:200

免疫细胞化学 (ICC/IF)

1:100-1:200

View 1 publication 1 publication

产品规格

种属反应

Dog, Chicken, Cat, Human, Mouse, Non-human primate, Pig, Rat

已发表种属

Human, Pig

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

R4A

抗原

Cytoskeletal smoothelin extract of chicken gizzard.

偶联物

Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

purified

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2239715

产品详细信息

OMA1-06020 detects smoothelin from human, canine, feline, porcine, chicken and monkey tissues. This antibody detects both the visceral and vascular smoothelin isoforms. Does not cross react with rat samples.

OMA1-06020 has been successfully used in Western blot, immunocytochemistry, and immunohistochemistry procedures. By Western blot, this antibody detects an ~59 kDa protein representing visceral smoothelin and an ~100 kDa protein representing vascular smoothelin from canine smooth muscle extracts. This antibody can be used to discriminate fully differentiated (contractile) smooth muscle cells from proliferative smooth muscle cells. Staining of formalin-fixed paraffin-embedded tissues requires boiling in 10 mM citrate buffer (pH 6.0) for 3x5 min, prior to immunostaining.

OMA1-06020 antigen is cytoskeletal smoothelin extract of chicken gizzard.

Store at 4°C, or in small aliquots at -20°C.

靶标信息

Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are a useful tool to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses reveal a broad species distribution of this protein. Cells with SMC-like characteristics, such as myofibroblasts, myoepithelial cells, and skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections, as well as transfection studies, show a filamentous organization of smoothelin that is different from that of desmin and vimentin. Smoothelin colocalizes with actin stress fibers. Two tissue specific isoforms have been identified: a 59 kDa isoform specific for visceral SMC; and a 100 kDa specific for vascular SMC. Human smoothelin is encoded by a single copy gene which is located on chromosome 22.

仅用于科研。不用于诊断过程。未经明确授权不得转售。