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SnRK2.2, SnRK2.3, SnRK2.6/Ser/Thr-protein kinase SnRK Polyclonal Antibody
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SnRK2.2, SnRK2.3, SnRK2.6/Ser/Thr-protein kinase SnRK Antibody (AS14-2783) in WB
20 µg of total protein from two-week-old seedling of Arabidopsis thaliana Col-0 (1), snrk 2.2/3/6 (2), snrk 1/4/5/7/8/9/10 (3) extracted with kinase buffer were separated on 10% SDS-PAGE and blotted 30 min to PVDF using semi-dry. Blots were blocked with 5% skim milk in TBST for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:5,000 in 0.5% TBST for o... View More
产品信息
AS14-2783
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SnRK2.2, SnRK2.3, SnRK2.6.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
SRK2D, SRK2E and SRK2I encode closely related, plant-specific SNF1-related protein kinase 2 (SnRK2) family members in Arabidopsis thaliana that function primarily in stress hormone signaling; their gene products are soluble kinases found predominantly in the nucleus in relevant developmental contexts and are activated by abscisic acid (ABA) and/or osmotic stress. Structurally, these proteins comprise an N-terminal serine/threonine protein kinase catalytic domain coupled to a C-terminal regulatory region that is required for stimulus-dependent activation and protein-protein interactions; for SRK2E, the C-terminal regulatory region can be functionally subdivided into domains that differentially contribute to ABA-dependent versus ABA-independent (osmotic stress) activation and can mediate interaction with the PP2C phosphatase ABI1. Functionally, SRK2D/E/I act as major positive regulators of ABA signaling during water-deficit responses and seed physiology: SRK2E is required for rapid stomatal closure under decreased humidity and for full induction of ABA-responsive genes, while combined loss of SRK2D, SRK2E and SRK2I causes strong ABA insensitivity and drought susceptibility with broadly impaired ABA- and dehydration-induced transcription, consistent with their redundant control of downstream transcription factor phosphorylation and gene expression programs.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 41K; AT3G50500; AT4G33950; AT5G66880; ATOST1; F17I5.140; F17I5_140; OPEN STOMATA 1; OSKL2; OST1; P44; SNRK2-2; SNRK2-3; SNRK2-6; SNRK2.2; SNRK2.3; SNRK2.6; SPK-2-2; SRK2D; SRK2E; SRK2I
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