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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Sodium/Calcium Exchanger Antibodies

          Invitrogen

          Sodium/Calcium Exchanger Monoclonal Antibody (C2C12)

          高级验证
          27个已发表图片
          88 篇参考文献

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          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
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          Cite Sodium/Calcium Exchanger Monoclonal Antibody (C2C12)

          • 抗体检测数据 (6)
          • 已发表图片 (27)
          • 高级验证 (1)
          Sodium/Calcium Exchanger Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Sodium/Calcium Exchanger Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 34

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          Sodium/Calcium Exchanger Antibody (MA3-926) in ICC/IF

          Immunofluorescence analysis of Sodium/Calcium Exchanger was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Sodium/Calcium Exchanger Monoclonal Antibody (C2C12) (Product # MA3-926) at 1:200 dilution ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
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          查看产品

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          Sodium/Calcium Exchanger Antibody in Immunocytochemistry (ICC/IF)
          Sodium/Calcium Exchanger Antibody in Immunocytochemistry (ICC/IF)
          Sodium/Calcium Exchanger Antibody in Immunocytochemistry (ICC/IF)
          Sodium/Calcium Exchanger Antibody in Immunocytochemistry (ICC/IF)
          Sodium/Calcium Exchanger Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Sodium/Calcium Exchanger Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Sodium/Calcium Exchanger Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Sodium/Calcium Exchanger Antibody in Immunohistochemistry (IHC)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody in Western Blot (WB)
          Sodium/Calcium Exchanger Antibody

          产品信息

          MA3-926

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:1,000
          查看67篇已发表文章 67篇已发表文章

          免疫组化 (IHC)

          -
          查看6篇已发表文章 6篇已发表文章

          免疫组化(石蜡) (IHC (P))

          1/100
          -

          免疫组化(冰冻) (IHC (F))

          Assay-dependent
          -

          免疫细胞化学 (ICC/IF)

          1:200
          查看12篇已发表文章 12篇已发表文章

          免疫沉淀 (IP)

          Assay-dependent
          查看1篇已发表文章 1篇已发表文章

          in situ PLA (PLA)

          -
          查看1篇已发表文章 1篇已发表文章

          其他PubMed (Misc)

          -
          查看1篇已发表文章 1篇已发表文章
          产品规格

          种属反应

          Dog, Guinea pig, Human, Mouse, Rabbit, Rat

          已发表种属

          Bovine, Dog, Guinea pig, Human, Marsupial, Mouse, Pig, Rabbit, Rat, Rodent

          宿主/亚型

          Mouse / IgM

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          C2C12

          抗原

          Purified canine cardiac sodium/calcium exchanger.

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          1 mg/mL

          纯化类型

          Affinity chromatography - MBP

          保存液

          PBS with 1mg/mL BSA

          内含物

          0.05% sodium azide

          保存条件

          -20°C, Avoid Freeze/Thaw Cycles

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2191111

          产品详细信息

          MA3-926 detects sodium/calcium exchanger from human, canine, rabbit, mouse, guinea pig and rat kidney and cardiac tissues.

          MA3-926 has been successfully used in Western blot, immunofluorescence, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a 120 kDa protein representing the sodium/calcium exchanger from guinea pig cardiac extract. The bands seen at 70 kDa and 160 kDa represent a proteolytic fragment and non-reduced exchanger respectively. MA3-926 is not recommended for Western blot procedures of rat tissues. Immunohistochemical staining of the sodium/calcium exchanger in rat heart with MA3-926 results in staining of the plasma membrane and intense staining of cardiac T-tubular membrane.

          The MA3-926 antigen is purified canine cardiac sodium/calcium exchanger. This antibody recognizes an epitope between amino acids 371-525, which is on the intracellular side of the plasma membrane.

          靶标信息

          Ca2+ plays a critical role in intracellular signaling and thus regulation of calcium balance is critical for the maintenance of this signaling function. The plasma membrane Na+/Ca2+ (NaCX) exchangers mediate Na+-dependent Ca2+ efflux in a wide variety of cell types. NaCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane. In mammals, at least 5 distinct genes code for the exchangers: Three NaCX (NaCX-1, NaCX-2, and NaCX-3), and two in the NaCKX family (NaCKX-1 and NaCKX-2). NaCX-1 is most prominently expressed in the heart where it plays a major role in excitation-contraction coupling (Matsuoka et al., 1993). NACX-1 may also be a target for protection of the heart and brain from ischemia and reperfusion injury (Hagihara et al., 2005; Bano et al., 2005; Choi, 2005).

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: cardiac sarcolemmal sodium-calcium exchanger; isoform NCX1.13; isoform NCX1.3; isoform NCX1.9; Na(+)/Ca(2+)-exchange protein 1; Na+/C2+ exchanger; Na+/Ca++ exchanger; Na+/Ca2+ exchanger; Na-Ca exchanger; Na/Ca exchanger; NKX; sodium-calcium exchanger; sodium/calcium exchanger; Sodium/calcium exchanger 1; sodium/calcium exchanger isoform NaCa13; sodium/calcium exchanger isoform NaCa3; sodium/calcium exchanger isoform NaCa7; sodium/calcium exchanger isoform NaCa9; solute carrier family 8 (sodium/calcium exchanger), member 1; Solute carrier family 8 member 1; solute carrier family 8, member 1

          查看更多 收起

          基因别名: AI852629; AV344025; CNC; D930008O12Rik; NACA; Ncx; NCX1; SLC8A1

          查看更多 收起

          UniProt ID: (Dog) P23685, (Human) P32418, (Mouse) P70414, (Rat) Q01728, (Guinea pig) P48766

          查看更多 收起

          Entrez Gene ID: (Dog) 475738, (Rabbit) 100328570, (Human) 6546, (Mouse) 20541, (Rat) 29715, (Guinea pig) 100135620

          查看更多 收起

          功能
          calcium:sodium antiporter activity calcium ion binding calmodulin binding ankyrin binding calcium:cation antiporter activity involved in regulation of postsynaptic cytosolic calcium ion concentration protein binding cytoskeletal protein binding ion channel binding antiporter activity metal ion binding calcium:sodium antiporter activity involved in regulation of cardiac muscle cell membrane potential secondary carrier transporter
          参与通路
          cellular sodium ion homeostasis cell communication positive regulation of bone mineralization sodium ion transmembrane transport response to muscle stretch calcium ion import calcium ion transmembrane transport sodium ion import across plasma membrane positive regulation of the force of heart contraction regulation of postsynaptic cytosolic calcium ion concentration regulation of the force of heart contraction regulation of heart rate regulation of sodium ion transport ion transport muscle contraction response to nutrient response to glucose regulation of cell communication by electrical coupling positive regulation of fibroblast migration regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion regulation of cardiac muscle contraction by calcium ion signaling vascular smooth muscle contraction telencephalon development response to ATP cellular response to reactive oxygen species response to immobilization stress response to drug response to hydrogen peroxide relaxation of smooth muscle negative regulation of cytosolic calcium ion concentration cardiac muscle cell development calcium ion homeostasis relaxation of cardiac muscle cardiac muscle contraction cytosolic calcium ion transport calcium ion transport into cytosol cellular response to caffeine cellular response to cAMP sodium ion export cellular response to hypoxia membrane depolarization during cardiac muscle cell action potential cell communication by electrical coupling involved in cardiac conduction sodium ion import calcium ion export regulation of cardiac conduction response to hypoxia embryonic placenta development heart morphogenesis transport sodium ion transport calcium ion transport cellular calcium ion homeostasis positive regulation of cytosolic calcium ion concentration post-embryonic development embryonic heart tube development muscle fiber development regulation of calcium ion transport transmembrane transport regulation of cardiac muscle cell membrane potential
          It has to be done as per old AB suggested Products section.
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