Invitrogen

Sorcin Polyclonal Antibody

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 篇参考文献

查看其他9个Sorcin抗体

说明书

实验方案

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说明书

实验方案

技术支持

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Sorcin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of Sorcin in human cell line U-251 MG shows localization to nucleoplasm and cytosol. Samples were probed using a Sorcin Polyclonal Antibody (Product # PA5-64975).

Sorcin Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of Sorcin in human cerebral cortex, liver, rectum and skeletal muscle using Sorcin Polyclonal Antibody (Product # PA5-64975) (A) shows similar protein distribution across tissues to an independent Sorcin Polyclonal Antibody (B).

Sorcin Antibody in Western Blot (WB) — Western blot analysis of Sorcin in human cell line RT-4 and human cell line U-251 MG. Samples were probed using a Sorcin Polyclonal Antibody (Product # PA5-64975).

Sorcin Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of Sorcin demonstrates antibody specificity. Immunohistochemical analysis of Sorcin using anti-Sorcin Polyclonal Antibody (Product # PA5-64975), shows significant staining of Sorcin in human rectum and shows minimal or weak staining in human skeletal muscle tissues. The relative expression levels of Sorcin within each tissue is shown using RNA-Seq. {RE}

产品信息

PA5-64975

应用	建议稀释比	已发表文章
免疫印迹 (WB)	0.04-0.4 µg/mL	-
免疫组化 (IHC)	-	查看1篇已发表文章 1篇已发表文章
免疫组化（石蜡） (IHC (P))	1:200-1:500	-
免疫细胞化学 (ICC/IF)	0.25-2 µg/mL	-

产品规格
种属反应	Human
已发表种属	Mouse
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Recombinant Human Sorcin 查看免疫原
偶联物	Unconjugated
形式	Liquid
浓度	0.05 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.2, with 40% glycerol
内含物	0.02% sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2662771

产品详细信息

Immunogen sequence: GGGYYPGGYGG APGGPAFPGQ TQDPLYGYFA AVAGQDGQID ADELQRCLTQ SGIAGGYK

Highest antigen sequence identity to the following orthologs - mouse 100%, rat 95%.

靶标信息

Sorcin is a 198 amino acid, soluble, resistance-related, calcium-binding cytosolic protein belonging to the penta-EF hand (PEF) family of calcium binding proteins and contains 4 EF-hand calcium-binding motifs. Human Sorcin is known to share 95% sequence identity with hamster Sorcin. It is known to associate with the sarcolemmal proteins Annexin VII, cardiac ryanodine receptors and L-type Ca2+ channels and thus influence the intracellular Ca (2+)-homeostasis. It is also important in the regulation of intracellular Ca2+ cycling and Ca2+ influx pathways in the heart and skeletal muscle during excitation-contraction. It is overproduced in many multi-drug resistance (MDR) cells and regulates cell apoptosis pathways, thus acting as a new MDR marker for prognosis of acute myeloid leukemia (AML) and a good target for anti-MDR drug development.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Insights into the Biology of Hearing and Deafness Revealed by Single-Cell RNA Sequencing.

Cell reports

Ranum PT,Goodwin AT,Yoshimura H,Kolbe DL,Walls WD,Koh JY,He DZZ,Smith RJH

PA5-64975 was used in Immunohistochemistry-immunofluorescence to provide insights into the expression profiles of these cells and document an unappreciated complexity in isoform variety in deafness-associated genes.

Tue Mar 12 00:00:00 EDT 2019

生物信息学

蛋白别名: 22 kDa protein; calcium binding protein amplified in mutlidrug-resistant cells; CP-22; CP22; H_RG167B05.1; Sorcin; V19

基因别名: CP-22; CP22; SCN; SRI; V19

UniProt ID: (Human) P30626

Entrez Gene ID: (Human) 6717

分子生物学功能: actin or actin-binding cytoskeletal protein

功能

action potential proteolysis transport calcium ion transport intracellular sequestering of iron ion regulation of striated muscle contraction signal transduction heart development muscle organ development regulation of heart contraction negative regulation of heart rate regulation of cell communication by electrical coupling regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum positive regulation of insulin secretion involved in cellular response to glucose stimulus cytoplasmic sequestering of transcription factor positive regulation of release of sequestered calcium ion into cytosol regulation of calcium ion transport negative regulation of ryanodine-sensitive calcium-release channel activity regulation of cardiac muscle cell contraction regulation of relaxation of muscle regulation of high voltage-gated calcium channel activity regulation of cell communication by electrical coupling involved in cardiac conduction negative regulation of transcription regulatory region DNA binding

参与通路

protease binding calcium-dependent cysteine-type endopeptidase activity receptor binding calcium channel regulator activity calcium ion binding protein binding ion channel binding protein heterodimerization activity repressing transcription factor binding

Sorcin Polyclonal Antibody

产品信息

PA5-64975

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

生物信息学

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