Bethyl Laboratories

Sp1 Polyclonal Antibody

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抗体检测数据 (5)

Sp1 Antibody in ChIP-Sequencing (ChIP-seq)

Sp1 Antibody in ChIP-Sequencing (ChIP-seq)

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Sp1 Antibody (A300-134A) in ChIP-seq

Localization of Sp1 Binding Sites by ChIP-sequencing. Chromatin from acute myeloid leukemia cell line was immunoprecipitated with anti-Sp1 antibody A300-134A and analyzed by DNA sequencing. The figure illustrates the peak distribution of Sp1 binding within a 250 Kb region of chromosome 6 as detected using anti-Sp1 A300-134A. ChIP-seq validation performed by Active Motif, Carlsbad, CA.

Sp1 Antibody in ChIP-Sequencing (ChIP-seq)

Sp1 Antibody in Immunohistochemistry (IHC)

Sp1 Antibody in Immunohistochemistry (IHC)

Sp1 Antibody in Western Blot (WB)

Sp1 Antibody in Western Blot (WB)

Product Image Gallery

Sp1 Antibody in ChIP-Sequencing (ChIP-seq) — Localization of Sp1 Binding Sites by ChIP-sequencing. Chromatin from acute myeloid leukemia cell line was immunoprecipitated with anti-Sp1 antibody A300-134A and analyzed by DNA sequencing. The figure illustrates the peak distribution of Sp1 binding within a 250 Kb region of chromosome 6 as detected using anti-Sp1 A300-134A. ChIP-seq validation performed by Active Motif, Carlsbad, CA.

Sp1 Antibody in Immunohistochemistry (IHC) — Detection of mouse SP1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SP1 (Cat. No. A300-134A Lot5) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.

Sp1 Antibody in Immunohistochemistry (IHC) — Detection of human SP1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SP1 (Cat. No. A300-134A Lot5) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.

Sp1 Antibody in Western Blot (WB) — Detection of human and mouse SP1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and mouse Renca cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SP1 antibody A300-134A (lot A300-134A-5) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Sp1 Antibody in Western Blot (WB) — Detection of human SP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SP1 antibody A300-134A (lot A300-134A-5) used for IP at 6 µg per reaction. SP1 was also immunoprecipitated by a previous lot of this antibody (A300-134A-4) and rabbit anti-SP1 antibody A303-944A. For blotting immunoprecipitated SP1, A300-134A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

产品信息

A300-134A

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000-1:10,000

-

免疫组化 (IHC)

1:2,000-1:10,000

-

免疫沉淀 (IP)

2-10 µg/mg lysate

-

染色质免疫沉淀测序 (ChIP-Seq)

4 µg/30 µg chromatin

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Region between residue 750 and the C-terminus (residue 785).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

phosphate/tris citrate, pH 7-8

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

产品详细信息

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

靶标信息

SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: HGNC:11205; Specificity protein 1; Transcription factor Sp1

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基因别名: 1110003E12Rik; AA450830; AI845540; SP1; Sp1-1; TSFP1

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UniProt ID: (Human) P08047, (Mouse) O89090

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Entrez Gene ID: (Human) 6667, (Mouse) 20683

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RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding core promoter sequence-specific DNA binding RNA polymerase II repressing transcription factor binding DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding protein binding protein C-terminus binding transcription factor binding histone acetyltransferase binding protein homodimerization activity histone deacetylase binding bHLH transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding metal ion binding HMG box domain binding RNA polymerase II core promoter sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding nucleic acid binding enhancer binding C2H2 zinc finger transcription factor

参与通路

regulation of transcription, DNA-templated viral process snRNA transcription from RNA polymerase II promoter positive regulation by host of viral transcription positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter rhythmic process regulation of phenotypic switching by transcription from RNA polymerase II promoter positive regulation of hydrogen sulfide biosynthetic process ossification in utero embryonic development trophectodermal cell differentiation liver development embryonic placenta development transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter megakaryocyte differentiation lung development enucleate erythrocyte differentiation embryonic camera-type eye morphogenesis embryonic skeletal system development embryonic process involved in female pregnancy definitive hemopoiesis