Invitrogen

Survivin Recombinant Rabbit Monoclonal Antibody (9H18L32)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1个已发表图片

1 篇参考文献

查看其他46个Survivin抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

Survivin Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 12

Survivin Antibody (700387) in ICC/IF

Immunofluorescence analysis of Survivin was performed using 70 % confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Survivin Recombinant Rabbit Monoclonal Antibody (9H18L32) (Product # 700387) at 5 µg/mL in 0.1% BSA and incubated for overnight at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluo... View More

Survivin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

产品信息

700387

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.25-1 µg/mL

免疫组化（石蜡） (IHC (P))

1 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Bovine, Hamster, Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

9H18L32

抗原

A recombinant protein corresponding to amino acids 1-142.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2532319

产品详细信息

This antibody is predicted to react with bovine and hamster based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Survivin (IAP4) is the smallest member of the Inhibitors of Apoptosis Protein (IAP) gene family. The IAPs are involved in multiple cell functions such as cell signaling, cell division, metabolism, and exhibits differential expression in nearly all human cancers, but not in most normal tissues. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but the Survivin gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of survivin’s gene expression. Survivin is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. Survivin may counteract a default induction of apoptosis in G2/M phase. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affect regulation of neural cell proliferative responses after brain injury. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Survivin Splice Variants in Arsenic Trioxide (As₂O₃)-Induced Deactivation of PI3K and MAPK Cell Signalling Pathways in MCF-7 Cells.

Genes

Laka K,Makgoo L,Mbita Z

700387 was used in Immunocytochemistry to explore the downstream effect of arsenic trioxide on survivin splice variants and consequent cytotoxicity.

Mon Jan 14 00:00:00 EST 2019