Invitrogen

Synaptotagmin 7 Polyclonal Antibody

Product Image Gallery

Synaptotagmin 7 Antibody in Western Blot (WB) — Figure S4 Modulating Coronavirus Egress, Related to Figure 4 (A) Extent of Arl8b depletion after siRNA treatment. Cell lysates probed with anti-Arl8b and anti-actin antibodies. (B) Quantification of Arl8b depletion from 4 independent experiments. (C) Effect of Rab27 depletion on MHV egress. TCID50/mL was calculated from extracellular media of non-target and Rab27a siRNA treated, MHV-infected cells (10hr pi). Mean data of 2 independent sets of experiments plotted. (D) Effect of GW4869 on MHV egress. Infected cells in serum-free media were treated with GW4869 (10 muM) or DMSO (8-14 hr pi); extracellular media and cell lysates collected at 14hrpi and MHV genomic RNA quantified. Mean data of 2 independent sets of experiments plotted. (E) Western blot of extracellular media from (D) probed with antibody against CD81, a marker for exosomes. (F) Effect of BAPTA-AM calcium chelation on MHV egress and replication. Infected cells treated with BAPTA-AM (30 muM) or DMSO (8-12 hr pi) in calcium-free media with EGTA. Mean data of 2 independent sets of experiments plotted. (G) Effect of Synaptotagmin VII depletion on MHV egress. Cells incubated with Synaptotagmin VII (50 nm) or non-target siRNA (50 nm) for 72 hr and infected with MHV. Extracellular medium and cell lysates collected at 14 hr pi. Mean data of 2 independent sets of experiments plotted. (H) Extent of Synaptotagmin VII depletion. Western blot of cell lysates from (G) probed with anti-synaptotagmin VII and anti-actin antibodies.

产品信息

PA5-50270

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:2,000	查看1篇已发表文章 1篇已发表文章
免疫组化（石蜡） (IHC (P))	1:15-1:50	-

产品规格
种属反应	Human
已发表种属	Not Applicable
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Fusion protein corresponding to a region derived from internal residues of human synaptotagmin VII
偶联物	Unconjugated
形式	Liquid
浓度	0.5 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.4, with 40% glycerol
内含物	0.05% sodium azide
保存条件	-20°C
RRID	AB_2635723

产品详细信息

The antibody detects endogenous levels of total SYT7 protein.

靶标信息

This gene is a member of the synaptotagmin gene family and encodes a protein similar to other family members that mediate calcium-dependent regulation of membrane trafficking in synaptic transmission. A similar protein in rodents mediates hormone secretion and lysosome exocytosis. In humans, expression of this gene has been associated with prostate cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

β-Coronaviruses Use Lysosomes for Egress Instead of the Biosynthetic Secretory Pathway.

Cell

Ghosh S,Dellibovi-Ragheb TA,Kerviel A,Pak E,Qiu Q,Fisher M,Takvorian PM,Bleck C,Hsu VW,Fehr AR,Perlman S,Achar SR,Straus MR,Whittaker GR,de Haan CAM,Kehrl J,Altan-Bonnet G,Altan-Bonnet N

Published figure using Synaptotagmin 7 polyclonal antibody (Product # PA5-50270) in Western Blot

Thu Dec 10 00:00:00 EST 2020

生物信息学

蛋白别名: IPCA-7; Prostate cancer-associated protein 7; Synaptotagmin VII; Synaptotagmin-7; SYT-7; SytVII

基因别名: IPCA-7; IPCA7; PCANAP7; SYT-VII; SYT7; SYTVII

UniProt ID: (Human) O43581

Entrez Gene ID: (Human) 9066

分子生物学功能: membrane trafficking regulatory protein

功能

plasma membrane repair vesicle fusion phagocytosis regulation of calcium ion-dependent exocytosis synaptic vesicle recycling positive regulation of calcium ion-dependent exocytosis regulation of bone remodeling calcium ion-regulated exocytosis of neurotransmitter regulation of phagocytosis regulation of insulin secretion regulation of glucagon secretion vesicle-mediated cholesterol transport phagosome-lysosome fusion short-term synaptic potentiation calcium ion regulated lysosome exocytosis

参与通路

calcium ion binding protein binding calmodulin binding calcium-dependent phospholipid binding phosphatidylinositol-4,5-bisphosphate binding syntaxin binding clathrin binding

Synaptotagmin 7 Polyclonal Antibody

产品信息

PA5-50270

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!