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Rockland
T7 RNA Polymerase Polyclonal Antibody
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T7 RNA Polymerase Antibody (200-401-MX5) in WB
Western Blot Results of Rabbit Anti-T7 RNA Polymerase Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB210-0500) [10 µL]. Lane 2: T4 RNA Ligase [0.5 µg]. Lane 3: T7 RNA Polymerase [0.5 µg]. Lane 4: Bovine Albumin (BSA) (p/n 001-0133) [0.5 µg]. Primary Antibody: Rabbit Anti-T7 RNA Polymerase Antibody at 1.0 µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG Peroxidase Conjugated (p/n 611-1302) at 1: 40,000 at RT for 30 mins. Block: BlockOut Universal Blocking Buffer (p/n MB-073) at RT for 1 hr. Expected MW: ~... View More
产品信息
200-401-MX5
产品规格
种属反应
Virus
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Anti-T7 Polymerase antibody was produced from whole rabbit serum produced by repeated immunizations with T7 Polymerase isolated from E. coli.
偶联物
形式
Liquid
浓度
1.19 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2
内含物
0.01% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
靶标信息
T7 RNA polymerase is a DNA-dependent RNA polymerase enzyme derived from the T7 bacteriophage. It is commonly used in molecular biology research and biotechnology applications. Expressed and purified from E. coli, T7 RNA polymerase functions by recognizing and binding to the T7 promoter sequence, initiating transcription of RNA from the corresponding DNA template. It has a single-subunit structure with distinct functional domains, including a DNA-binding domain, a catalytic domain, and a C-terminal domain. T7 RNA polymerase is highly processive, allowing for the synthesis of long RNA molecules without dissociating from the DNA template.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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