Invitrogen

TAB1 Recombinant Rabbit Monoclonal Antibody (21H11L15)

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TAB1 Antibody (703255) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), SK-BR-3 (Lane 2), T-47D (Lane 3), HeLa (Lane 4), RAW 264.7 (Lane 5), HEK-293 (Lane 6), A-431 (Lane 7) and NIH3T3 (Lane 8). The blot was probed with Anti-TAB1 Recombinant Rabbit Monoclonal Antibody (Product # 703255, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:5000 dilution). A ~55 kDa band corresponding to TAB1 was observed across... View More

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TAB1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. HeLa cells were transfected with TAB1 siRNA and a decrease in signal intensity was observed in Western blot application using Anti-TAB1 Recombinant Rabbit Monoclonal Antibody (Product # 703255). {KD}

产品信息

703255

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

21H11L15

抗原

Protein corresponding to human TAB1 [aa1-aa370]

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2784571

产品详细信息

This antibody is predicted to react with Monkey, Pig, Dog.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

TGF-beta-activated kinase 1 and MAP3K7-binding protein 1 (TAB1) is a regulator of the MAP kinase kinase kinase MAP3K7/TAK1, which is known to mediate various intracellular signaling pathways, such as those induced by TGF beta, interleukin 1, and WNT-1. TAB1 interacts and thus activates TAK1 kinase. It has been shown that the C-terminal portion of TAB1 is sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF beta, suggesting that this protein may function as a mediator between TGF beta receptors and TAK1. It can also interact with and activate the mitogen-activated protein kinase 14 (MAPK14/p38alpha), and thus represents an alternative activation pathway, in addition to the MAPKK pathways, which contributes to the biological responses of MAPK14 to various stimuli.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: beta activated kinase-1 binding protein-1; MGC57664; mitogen activated protein kinase kinase kinase 7 interacting protein 1; mitogen-activated protein kinase kinase kinase 7 interacting protein 1; Mitogen-activated protein kinase kinase kinase 7-interacting protein 1; RP3-407F17.2; TAB1beta; TAK1-binding protein 1; TGF-beta-activated kinase 1 and MAP3K7-binding protein 1; TGF-beta-activated kinase 1-binding protein 1; transforming growth factor beta-activated kinase-binding protein 1

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基因别名: 2310012M03Rik; 3'-Tab1; b2b449Clo; MAP3K7IP1; TAB1

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UniProt ID: (Human) Q15750, (Mouse) Q8CF89

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Entrez Gene ID: (Human) 10454, (Mouse) 66513

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功能

protein serine/threonine phosphatase activity protein binding enzyme activator activity kinase activator activity catalytic activity protein complex binding mitogen-activated protein kinase p38 binding protein phosphatase

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