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Invitrogen
TAF13 Monoclonal Antibody (16TA-3C12)
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TAF13 Antibody (MA3-073) in WB
Western blot analysis of TAF13 was performed by loading 10-20 µg of the indicated nuclear lysates and 5 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BX10). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product # 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. TAF13 was detected at ~14 kDa using a TAF13 mouse monoclonal antibody (Product # MA3-073) at a dilution of 1:1000 in blocking buffer for 1... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA3-073
产品规格
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
16TA-3C12
抗原
Recombinant human TAF13 protein
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
Conc. Not Determined
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA3-073 detects TATA Binding protein (TBP) Associated factor at 14kDa in the human and mouse samples.
MA3-073 has been succesfully used in Western blot application.
The MA3-073 immunogen is a human recombinant human TAF13 protein.
靶标信息
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a small subunit associated with a subset of TFIID complexes. This subunit interacts with TBP and with two other small subunits of TFIID, TAF10 and TAF11. There is a pseudogene located on chromosome 6. [provided by RefSeq, Jul 2008].
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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