Invitrogen

TBK1 Monoclonal Antibody (108A429)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他18个TBK1抗体

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说明书

实验方案

技术支持

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TBK1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of TBK1 was performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TBK1 Monoclonal Antibody (108A429) (Product # MA1-20344) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with Hoechst 33342 (Product # H1399). F-actin (Panel c: Red) was stained with Alexa Fluor™ Plus 647 Phalloidin (Product # A30107, 1:2000 dilution). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 40 magnification.

TBK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of TBK1 in human testis tissue using TBK1 Monoclonal Antibody (108A429) (Product # MA1-20344).

TBK1 Antibody in Western Blot (WB) — Western blot was performed using Anti-TBK1 Monoclonal Antibody (108A429) (Product # MA1-20344) and a 83 kDa band corresponding to TBK1 was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), HeLa (Lane 2), K-562 (Lane 3), MCF7 (Lane 4), T-47D (Lane 5) and SK-BR-3 (Lane 6), tissue extracts of Mouse Heart (Lane 8), Rat Heart (Lane 7), Mouse Testis (Lane 9) and Rat Testis (Lane 10) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001) and then blocked with Pierce™ Protein-Free T20 (PBS) Blocking Buffe (Product # 37573). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

TBK1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK-293 cells were transfected with TBK1 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-TBK1 Monoclonal Antibody (108A429) (Product # MA1-20344). {KD}

产品信息

MA1-20344

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1-2 µg/mL	-
免疫组化（石蜡） (IHC (P))	1:20-1:1,000	-
免疫细胞化学 (ICC/IF)	1:20-1:100	-

产品规格
种属反应	Bovine, Dog, Human, Mouse, Rat
宿主/亚型	Mouse / IgG1
分类	Monoclonal
类型	Antibody
克隆号	108A429
抗原	A synthetic peptide corresponding to amino acids 563-577 (YYQFKKDKAERRLAYC) of human TBK1 was used as immunogen for this antibody (NP_037386)
偶联物	Unconjugated
形式	Liquid
浓度	1.0 mg/mL
纯化类型	Protein G
保存液	PBS
内含物	0.05% sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_559588

产品详细信息

MA1-20344 detects NAK in human samples.

靶标信息

Nuclear factor kappa B (NF-KB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-KB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-KB is associated with IKB proteins in the cell cytoplasm, which inhibit NF-KB activity. Phosphorylation of I-KB by IKB kinase (IKK) complex leads to degradation of I-KB and activation of NF-KB. The IKK complex contains IKKa, IKKß, and IKKK. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-KB-activating kinase), and T2K. NAK/TBK1 activates IKKß through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-kB activation in response to growth factors. NAK/TBK1 functions upstream of NIK and the IKK complex. NAK/TBK1 is also critical in protecting embryonic liver from apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: FLJ11330; NF-kappa-B-activating kinase; NF-kB-activating kinase; serine/threonine protein kinase TBK1; Serine/threonine-protein kinase TBK1; T2K; TANK-binding kinase 1; tbk

基因别名: 1200008B05Rik; AI462036; AW048562; FTDALS4; NAK; T2K; TBK1

UniProt ID: (Human) Q9UHD2, (Mouse) Q9WUN2

Entrez Gene ID: (Dog) 481145, (Human) 29110, (Bovine) 533141, (Mouse) 56480, (Rat) 299827

分子生物学功能: non-receptor serine/threonine protein kinase protein modifying enzyme