Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
TCF7 Recombinant Rabbit Monoclonal Antibody (18H15L9)
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 5
TCF7 Antibody (702957) in ICC/IF
Knockdown of TCF7 was achieved by transfecting HeLa cells with specific siRNA (Silencer® select Product # s13877, s13878). Immunofluorescence analysis was performed on HeLa cells (untransfected, panel a-d), transfected with TCF7 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labeled with Anti-TCF Recombinant Rabbit Monoclonal Antibody (Product # 702957, 1:100 dilution), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (P... View More
产品信息
702957
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
18H15L9
抗原
Protein corresponding to human TCF7 [aa1-aa384]
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2784552
产品详细信息
This antibody is predicted to react with Monkey, Cat, Bovine.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
TCF7 (transcriptional factor 7) is a transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4+ and CD8+ immature thymocytes. Isoforms for TCF7 lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. TCF7 binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. It may also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3, and TLE4 repress transactivation mediated by TCF7 and CTNNB1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: HMG-box; OTTHUMP00000159390; OTTHUMP00000159391; T-cell factor 1; T-cell specific; T-cell-specific transcription factor 1; TCF-7; Transcription factor 7
基因别名: TCF-1; TCF1; TCF7
UniProt ID: (Human) P36402
Entrez Gene ID: (Human) 6932
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
immune response
neural tube development
embryonic genitalia morphogenesis
T cell receptor V(D)J recombination
regulation of cell proliferation
canonical Wnt signaling pathway involved in negative regulation of apoptotic process
alpha-beta T cell differentiation
embryonic digestive tract morphogenesis
embryonic hindgut morphogenesis
canonical Wnt signaling pathway
cellular response to interleukin-4
beta-catenin-TCF complex assembly
