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Invitrogen
-Flow-20170216130641.jpg "TCR H-Y (male antigen) Antibody (12-9930-81) in Flow")
Description: The T3.70 monoclonal antibody reacts with the transgenic alpha betaTCR that recognizes the male antigen H-Y in the context of H-2D^b. A large fraction of T cells in H-Y TCR transgenic mouse expresses this receptor. The H-Y TCR transgenic mouse has been used extensively to study T cell development and the role of thymic major histocompatibility complex in CD4^+ and CD8^+ T cell differentiation.
Applications Reported: The T3.70 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The T3.70 antibody has been tested by flow cytometric analysis of CD11.3 line, which is derived from a thymic tumor of the H-Y TCR transgenic mice and expresses the transgenic H-Y TCR. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
A large fraction of T cells in H-Y TCR transgenic mouse expresses this receptor. The H-Y TCR transgenic mouse has been used extensively to study T cell development and the role of thymic major histocompatibility complex in CD4+ and CD8+ T cell differentiation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: Male antigen HY; TCR HY
