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产品信息
11-5792-41
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
流式细胞分析 (Flow) |
5 µL (1 µg)/test | 查看1篇已发表文章 1篇已发表文章 |
| 产品规格 | |
|---|---|
种属反应 |
Human |
已发表种属 |
Human |
宿主/亚型 |
Mouse / IgG2b, kappa |
推荐的同型对照 |
Mouse IgG2b kappa Isotype Control (eBMG2b), FITC, eBioscience™ |
分类 |
Monoclonal |
类型 |
Antibody |
克隆号 |
H131 |
偶联物 |
FITC |
激发/发射光谱 |
498/517 nm
查看光谱
|
形式 |
Liquid |
浓度 |
5 µL/Test |
纯化类型 |
Affinity chromatography |
保存液 |
PBS, pH 7.2, with 0.2% BSA |
内含物 |
0.09% sodium azide |
保存条件 |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_1944415 |
产品详细信息
Description: This H131 monoclonal antibody recognizes the human T cell receptor (TCR) Vbeta13.1 allele. Composed of an alpha and beta chain, TCR specificity is typically determined by Valpha, Jalpha, Vbeta, Dbeta, and Jbeta gene rearrangement. Vbeta expression in humans has been examined in studies on the effects of superantigens, inflammation, autoimmune disease, and HIV infection. More recently, assessment of TCR Vbeta expression has been used to phenotype T cell clonality in CD3+/TCRalpha beta+ large granular lymphocyte leukemias. A member of the Ig superfamily, this receptor is expressed on a subset of peripheral blood T cells.
Applications Reported: This H131 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This H131 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
Flow Cytometry
Panel Builder
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: TCR Vb13.1; TCRV beta-13.1
