Invitrogen

TER-119 Monoclonal Antibody (TER-119), Super Bright™ 600, eBioscience™

TER-119 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 17

TER-119 Antibody (63-5921-82) in Flow

C57BL/6 mouse bone marrow cells were stained with CD45 Monoclonal Antibody, FITC (Product # 11-0451-82) and 0.25 µg of Rat IgG2b kappa Isotype Control, Super Bright 600 (Product # 63-4031-82) (left) or 0.25 µg of TER-119 Monoclonal Antibody, Super Bright 600 (right). Total viable cells were used for analysis.

Product Image Gallery

TER-119 Antibody in Western Blot (WB) — Figure 1. Baseline characterization of RBC-extracellular vesicles (EVs) in transgenic murine model. (A) Schematic representation of the experimental murine models. Erythroid lineage specific conditional Cre knock-in mice (EpoR-Cre mice, BALB/c background) were crossed with membrane-targeted Tomato/membrane-targeted GFP (mTmG Rosa26 mice, C57BL6/Sv129) to produce double transgenic (EpoR-Cre/mTmG) off-springs. Activated Cre mediates the removal of membrane-targeted tandem dimer Tomato (mT) sequence allowing the expression of membrane-targeted green fluorescent protein (mG) in RBCs. Arrows represent the direction of transcription. Triangles represent loxP sites for Cre-mediated recombination. pA stands for polyadenylation sequences. (B) High-magnification fluorescence images (63x) of fresh prepared peripheral blood smear and cell-free plasma smear from mTmG and EpoR-Cre/mTmG mice to characterize the mature circulating RBCs, and RBC-EVs (n = 4 in each group). Small bright GFP positive dots in the peripheral blood smear represent platelets. Scale bar: 10 mum. (C) Nanoflow cytometric characterization of cell-free plasma from mTmG mice and EpoR-Cre/mTmG mice, showing presence of mGFP + RBC-EVs in EpoR-Cre/mTmG mouse plasma. The auto fluorescence from the Suspension Buffer (Phosphate Buffer Saline) has been accounted for, as background. Shown are Td on the y-axis and GFP in the x-axis and divided in four quadrants (Q1 is mTd+ [red]; Q2 is mTd+/mGFP+ [double positive]; Q3 is mGFP+ [gr

Product Details

63-5921-82

Applications

Tested Dilution

Publications

免疫印迹 (WB)

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流式细胞分析 (Flow)

0.5 µg/test

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产品规格

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 600, eBioscience™

分类

Monoclonal

类型

Antibody

克隆号

TER-119

Conjugate

Super Bright™ 600

Excitation/Emission Max

414/601 nm View spectra

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

RRID

AB_2688117

Product Specific Information

Description: The TER-119 monoclonal antibody reacts with mouse erythroid cells from early proerythroblast to mature erythrocyte stages. The TER-119 antigen is present in yolk sac, fetal and newborn liver, but is not expressed by cells carrying BFU-E and CFU-E activities. Several erythroleukemia cell lines tested so far are negative for expression of TER-119 antigen even after dimethylsulfoxide stimulation. Biochemical and molecular analysis of the TER-119 antigen indicate that this molecule is associated with the surface glycophorin A, but is not a typical glycophorin.

Applications Reported: This TER-119 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TER-119 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

TER-119 is a lineage marker for erythroid cells from early proerythroblast to mature erythrocyte stages in adult blood, spleen, and bone marrow. It is also present in yolk sac, and fetal and newborn liver. The TER-119 antigen is not expressed by cells of earlier erythroid development at BFU-e (blast-forming unit erythroid) stage or CFU-e (colony-forming unit erythroid) stage.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Flow Cytometry

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Bioinformatics

Protein Aliases: Ly-76; Ly76; TER119

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TER-119 Monoclonal Antibody (TER-119), Super Bright™ 600, eBioscience™

TER-119 Antibody (63-5921-82) in Flow

Product Details

63-5921-82

免疫印迹 (WB)

流式细胞分析 (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

分类

类型

克隆号

Conjugate

Excitation/Emission Max

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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