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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • TER-119 Antibodies

          Invitrogen

          TER-119 Monoclonal Antibody (TER-119), Super Bright™ 600, eBioscience™

          16 Published Figures
          11 References
          View all (33) TER-119 antibodies
          Datasheet
          Protocols
          Tech Support

          Cite TER-119 Monoclonal Antibody (TER-119), Super Bright™ 600, eBioscience™

          Datasheet
          Protocols
          Tech Support
          • Antibody Testing Data (1)
          • Published Figures (16)
          TER-119 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 17

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          TER-119 Antibody (63-5921-82) in Flow

          C57BL/6 mouse bone marrow cells were stained with CD45 Monoclonal Antibody, FITC (Product # 11-0451-82) and 0.25 µg of Rat IgG2b kappa Isotype Control, Super Bright 600 (Product # 63-4031-82) (left) or 0.25 µg of TER-119 Monoclonal Antibody, Super Bright 600 (right). Total viable cells were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          View Product

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          Product Details

          63-5921-82

          Applications
          Tested Dilution
          Publications

          免疫印迹 (WB)

          -
          查看1篇已发表文章 1篇已发表文章

          流式细胞分析 (Flow)

          0.5 µg/test
          查看10篇已发表文章 10篇已发表文章
          产品规格

          Species Reactivity

          Mouse

          Published species

          Not Applicable

          Host/Isotype

          Rat / IgG2b, kappa

          Recommended Isotype Control

          Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 600, eBioscience™

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          TER-119

          Conjugate

          Super Bright™ 600
          • Alexa Fluor 660 
          • Alexa Fluor 700 
          • APC 
          • APC-eFluor 780
          • Biotin 
          • Brilliant UV 395
          • Brilliant UV 615
          • Brilliant UV 661
          • Brilliant UV 737
          • Brilliant UV 805
          • Brilliant Violet 421
          • Brilliant Violet 480
          • eFluor 450
          • eFluor 506
          • FITC
          • Functional Grade
          • PE 
          • PE-Cyanine5
          • PE-Cyanine5.5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-Cyanine5.5
          • PerCP-eFluor 710
          • Super Bright 436
          • Super Bright 645
          • Super Bright 702
          • Super Bright 780
          • Unconjugated

          Excitation/Emission Max

          414/601 nm View spectra spectra

          形式

          Liquid

          浓度

          0.2 mg/mL

          纯化类型

          Affinity chromatography

          保存液

          PBS, pH 7.2, with BSA

          内含物

          0.09% sodium azide

          保存条件

          4° C, store in dark, DO NOT FREEZE!

          RRID

          AB_2688117

          Product Specific Information

          Description: The TER-119 monoclonal antibody reacts with mouse erythroid cells from early proerythroblast to mature erythrocyte stages. The TER-119 antigen is present in yolk sac, fetal and newborn liver, but is not expressed by cells carrying BFU-E and CFU-E activities. Several erythroleukemia cell lines tested so far are negative for expression of TER-119 antigen even after dimethylsulfoxide stimulation. Biochemical and molecular analysis of the TER-119 antigen indicate that this molecule is associated with the surface glycophorin A, but is not a typical glycophorin.

          Applications Reported: This TER-119 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This TER-119 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

          When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

          Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

          Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

          Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser

          Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

          Target Information

          TER-119 is a lineage marker for erythroid cells from early proerythroblast to mature erythrocyte stages in adult blood, spleen, and bone marrow. It is also present in yolk sac, and fetal and newborn liver. The TER-119 antigen is not expressed by cells of earlier erythroid development at BFU-e (blast-forming unit erythroid) stage or CFU-e (colony-forming unit erythroid) stage.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          Flow Cytometry
          Panel Builder

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: Ly-76; Ly76; TER119

          View more View less

          It has to be done as per old AB suggested Products section.
          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

          Contact tech support
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