Invitrogen

TET1 Monoclonal Antibody (GT1462)

Product Image Gallery

TET1 Antibody in Immunocytochemistry (ICC/IF) — TET1 Monoclonal Antibody (GT1462) detects TET1 protein at nucleus by immunofluorescent analysis. Sample: TET1-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min. Green: TET1 protein stained by TET1 antibody (Product # MA5-16312) diluted at 1:1,000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

TET1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — TET1 Monoclonal Antibody (GT1462) detects TET1 protein at nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. TET1 Monoclonal Antibody (GT1462) (Product # MA5-16312) dilution: 1:100. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

TET1 Antibody in Western Blot (WB) — TET1 Monoclonal Antibody (GT1462) detects TET1 protein by western blot analysis. A. 50 µg whole cell lysate/extract from 293T cells transfected with scramble siRNA. B. 50 µg whole cell lysate/extract from TET1-knockdowned 293T cells.6% SDS-PAGE. TET1 Monoclonal Antibody (GT1462) (Product # MA5-16312) dilution: 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

TET1 Antibody in Immunohistochemistry (IHC) — 1 Fig. TET1 expression is elevated in patients with CCA. (A) Heat map for the expression of 2-OG-dependent enzymes was generated using TCGA data, which include 9 normal samples, 6 CCA tumors with IDH1 mutation, and 30 CCA tumors with WT IDH1. (B) The association of molecular signaling pathways and functions with alpha-KG-dependent enzymes. (C) Relative TET1, TET2, and TET3 mRNA expression levels were analyzed in human normal bile ducts (n = 9) and patients with CCA (n = 36) using TCGA data. (D) Representative TET1 immunohistochemical images are shown in noncancerous bile duct (a) and CCA samples (b-d). (E) Semiquantification of TET1 immunohistochemical staining data was calculated using Image J software. ** P < 0.01, *** P < 0.001 compared with relevant control.

TET1 Antibody in Western Blot, Immunoprecipitation (WB, IP) — 5 Figure miR-376a directly targeted TET1 and HIF1A. (A) Bioinformatics prediction (TargetScan database) of putative binding site at 3'-UTRs of TET1 and HIF-1A by miR-376a. (B and C) Luciferase activity was measured in HGC-27 cells transfected with HIF-1A or TET1 3'-UTR-fused pGL3 vector plus NC mimic, miR-376a mimic, NC inhibitor, miR-376a inhibitor. * p < 0.05; ** p < 0.01. (D) RT-qPCR showed miR-376a, TET1, HIF-1A expression levels in HGC-27 cells transfected with NC mimic, miR-376a mimic, NC inhibitor, miR-376a inhibitor. * p < 0.05; ** p < 0.01. (E) Co-immunoprecipitation showed interaction of TET1 with HIF-1A in HGC-27 cells. IgG served as negative control. (F) RT-qPCR showed PD-L1 mRNA levels in HGC-27 cells transfected with shNC, shHIF-1A, shTET1, shHIF-1A plus shTET1. ** p < 0.01. (G) Flow cytometry analysis showed PD-L1 protein levels in HGC-27 cells transfected with shNC, shHIF-1A, shTET1, shHIF-1A plus shTET1

TET1 Antibody in ChIP assay (ChIP) — Published figure using TET1 monoclonal antibody (Product # MA5-16312) in ChIP assay

产品信息

MA5-16312

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:3,000	查看2篇已发表文章 2篇已发表文章
免疫组化 (IHC)	-	查看1篇已发表文章 1篇已发表文章
免疫组化（石蜡） (IHC (P))	1:100-1:1,000	-
免疫细胞化学 (ICC/IF)	1:100-1:1,000	-
免疫沉淀 (IP)	1:100-1:500	查看2篇已发表文章 2篇已发表文章
染色质免疫沉淀实验 (ChIP)	Assay-dependent	查看1篇已发表文章 1篇已发表文章

产品规格
种属反应	Human, Mouse
已发表种属	Human, Mouse
宿主/亚型	Mouse / IgG2b
分类	Monoclonal
类型	Antibody
克隆号	GT1462
抗原	Recombinant protein encompassing a sequence within the center region of human TET1.
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Protein G
保存液	PBS
内含物	no preservative
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2537831

产品详细信息

Recommended positive controls: 293T, DDDDK-human TET1-transfected 293T, NT2D1, NT2D1 nuclear extract.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Ten-Eleven Translocation 1 Promotes Malignant Progression of Cholangiocarcinoma With Wild-Type Isocitrate Dehydrogenase 1.

Hepatology (Baltimore, Md.)

Bai X,Zhang H,Zhou Y,Nagaoka K,Meng J,Ji C,Liu D,Dong X,Cao K,Mulla J,Cheng Z,Mueller W,Bay A,Hildebrand G,Lu S,Wallace J,Wands JR,Sun B,Huang CK

Published figure using TET1 monoclonal antibody (Product # MA5-16312) in Immunohistochemistry

Sat May 01 00:00:00 EDT 2021

LncRNA NUTM2A-AS1 positively modulates TET1 and HIF-1A to enhance gastric cancer tumorigenesis and drug resistance by sponging miR-376a.

Cancer medicine

Wang J,Yu Z,Wang J,Shen Y,Qiu J,Zhuang Z

MA5-16312 was used in Western Blotting to elucidate the molecular mechanism of the role of NUTM2A-AS1 in gastric cancer.

Tue Dec 01 00:00:00 EST 2020

LncRNA NUTM2A-AS1 positively modulates TET1 and HIF-1A to enhance gastric cancer tumorigenesis and drug resistance by sponging miR-376a.

Cancer medicine

Wang J,Yu Z,Wang J,Shen Y,Qiu J,Zhuang Z

MA5-16312 was used in Western Blotting to elucidate the molecular mechanism of the role of NUTM2A-AS1 in gastric cancer.

Tue Dec 01 00:00:00 EST 2020

LncRNA NUTM2A-AS1 positively modulates TET1 and HIF-1A to enhance gastric cancer tumorigenesis and drug resistance by sponging miR-376a.

Cancer medicine

Wang J,Yu Z,Wang J,Shen Y,Qiu J,Zhuang Z

MA5-16312 was used in Western Blotting to elucidate the molecular mechanism of the role of NUTM2A-AS1 in gastric cancer.

Tue Dec 01 00:00:00 EST 2020

Ligand-induced gene activation is associated with oxidative genome damage whose repair is required for transcription.

Proceedings of the National Academy of Sciences of the United States of America

Sengupta S,Wang H,Yang C,Szczesny B,Hegde ML,Mitra S

MA5-16312 was used in Immunoprecipitation to show induction of oxidized bases and single-strand breaks (SSBs), but not direct double-strand breaks (DSBs), in the genome during gene activation by ligands of the nuclear receptor superfamily.

Tue Sep 08 00:00:00 EDT 2020

Integrin α6 signaling induces STAT3-TET3-mediated hydroxymethylation of genes critical for maintenance of glioma stem cells.

Oncogene

Herrmann A,Lahtz C,Song J,Aftabizadeh M,Cherryholmes GA,Xin H,Adamus T,Lee H,Grunert D,Armstrong B,Chu P,Brown C,Lim M,Forman S,Yu H

MA5-16312 was used in Western Blotting to conclude that ECM- integrin α6-STAT3-TET3 axis regulates hydroxymethylation of genes important for GSCs, thereby increasing GSC tumorigenicity and resistance to therapies.

Sun Mar 01 00:00:00 EST 2020

Hemodynamic disturbed flow induces differential DNA methylation of endothelial Kruppel-Like Factor 4 promoter in vitro and in vivo.

Circulation research

Jiang YZ,Jiménez JM,Ou K,McCormick ME,Zhang LD,Davies PF

Published figure using TET1 monoclonal antibody (Product # MA5-16312) in ChIP assay

Fri Jun 20 00:00:00 EDT 2014

生物信息学

蛋白别名: CXXC finger 6; CXXC zinc finger 6; CXXC-type zinc finger protein 6; Leukemia-associated protein with a CXXC domain; Methylcytosine dioxygenase TET1; RP11-119F7.1; Ten-eleven translocation 1 gene protein; ten-eleven translocation 1 gene protein homolog; ten-eleven translocation-1; tet oncogene 1; TET1 splice variant VP_DE4; TET1 splice variant VP_DE456

基因别名: 2510010B09Rik; AA517754; bA119F7.1; BB001228; CXXC6; D10Ertd17e; KIAA1676; LCX; mKIAA1676; MLL-TET1; TET1; TET1-MLL

UniProt ID: (Human) Q8NFU7

Entrez Gene ID: (Human) 80312, (Mouse) 52463

功能

inner cell mass cell differentiation transcription, DNA-templated regulation of transcription, DNA-templated protein O-linked glycosylation chromatin modification stem cell population maintenance regulation of DNA methylation positive regulation of transcription from RNA polymerase II promoter oxidation-reduction process DNA demethylation negative regulation of methylation-dependent chromatin silencing regulation of genetic imprinting positive regulation of cell proliferation covalent chromatin modification positive regulation of histone methylation

参与通路

iron ion binding zinc ion binding structure-specific DNA binding methylcytosine dioxygenase activity DNA binding protein binding oxidoreductase activity metal ion binding dioxygenase activity

TET1 Monoclonal Antibody (GT1462)

产品信息

MA5-16312

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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TET1 Monoclonal Antibody (GT1462)

产品信息

MA5-16312

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀 实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Brands

Shopping Tools

eSolutions

Support

我们的公司

Your items have has been added!

染色质免疫沉淀实验 (ChIP)