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TFAM Antibody (MA5-35365) in IHC (P)
Immunohistochemistry analysis of TFAM in paraffin-embedded human esophageal cancer. Samples were incubated with TFAM Monoclonal antibody (Product # MA5-35365) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
产品信息
MA5-35365
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
4H2M9
抗原
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TFAM (NP_0031921)
偶联物
形式
Liquid
浓度
0.20 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
内含物
0.05% ProClin 300
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2849266
产品详细信息
Immunogen sequence: QDAYRAEWQV YKEEISRFKE QLTPSQIMSL EKEIMDKHLK RKAMTKKKEL TLLGKPKRPR SAYNVYVAER FQEAKGDSPQ EKLKTVKENW KNLSDSEKEL Y
靶标信息
Mitochondrial transcription factor A (TFAM) is a crucial protein encoded by nuclear genes, but it is transported from the cytoplasm to mitochondria where it plays a key role in the maintenance and expression of mitochondrial DNA (mtDNA). It regulates the replication and transcription of mtDNA, ensuring the proper functioning of mitochondrial processes. Structurally, TFAM contains a molecular region known as the LC3 interacting region (LIR) motif, which allows it to bind to autophagy proteins such as LC3. This interaction is essential for the autolysosomal pathway, helping to eliminate leaked mtDNA and limit inflammation. TFAM levels vary across tissues, with notable effects on mitochondrial function; high levels can repress mtDNA expression in certain tissues, such as skeletal muscle, and contribute to deficiencies in oxidative phosphorylation (OXPHOS). Conversely, in other tissues like the heart, increased mtDNA copy number maintains a balanced TFAM-to-mtDNA ratio, preserving OXPHOS capacity. TFAM is integral to the packaging, stability, and replication of the mitochondrial genome, and disruptions in TFAM can have severe effects, including implications in neurodegeneration and other diseases related to mtDNA stress.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Mitochondrial transcription factor 1; mitochondrial transcription factor A; MtTF1; mtTFA; TCF-6; Testis-specific high mobility group protein; testis-specific HMG-box protein m-tsHMG; Transcription factor 6; transcription factor 6-like 1; Transcription factor 6-like 2; transcription factor 6-like 2 (mitochondrial transcription factor); transcription factor 6-like 3; Transcription factor A, mitochondrial; TS-HMG
基因别名: AI661103; Hmgts; MTTF1; MTTFA; TCF6; TCF6L1; TCF6L2; TCF6L3; TFAM; tsHMG
UniProt ID: (Human) Q00059, (Rat) Q91ZW1, (Mouse) P40630
Entrez Gene ID: (Human) 7019, (Rat) 83474, (Mouse) 21780
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription coactivator binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
DNA binding, bending
heat shock protein binding
poly(A) RNA binding
mitochondrial light strand promoter sense binding
DNA binding
mitochondrial DNA replication
chromatin remodeling
regulation of transcription, DNA-templated
transcription from mitochondrial promoter
transcription initiation from mitochondrial promoter
mitochondrial respiratory chain complex assembly
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
DNA-dependent DNA replication
regulation of transcription from RNA polymerase I promoter
transcription from RNA polymerase II promoter
mitochondrion organization
transcription, DNA-templated
