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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • TGF beta-1 Antibodies

          Invitrogen

          TGF beta-1 Monoclonal Antibody (F.888.7)

          高级验证
          9个已发表图片
          12 篇参考文献
          查看其他36个TGF beta-1抗体

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          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite TGF beta-1 Monoclonal Antibody (F.888.7)

          • 抗体检测数据 (5)
          • 已发表图片 (9)
          • 高级验证 (3)
          TGF beta-1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          TGF beta-1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          图: 1 / 17

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          TGF beta-1 Antibody (MA5-15065) in WB

          Western blot was performed using Anti-TGF beta-1 Monoclonal Antibody (F.888.7) (Product # MA5-15065) and 44-53 kDa bands corresponding to latent TGF beta1 was observed to increase in expression upon cisplatin treatment to MDA-MB-231 spheroids and differentiation & polarization of THP-1 to M2 macrophages. Whole cell extracts (30 µg lysate) of MDA-MB-231 spheroids(Lane 1), cisplatin treated MDA-MB-231 spheroids(Lane 2) and conditioned medium from MDA-MB-231 spheroids(Lane 3), cisplatin treated MDA-MB-231 spheroids(Lane 4) in Fig. a, and whol... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          TGF beta-1 Antibody in Western Blot (WB)
          TGF beta-1 Antibody in Western Blot (WB)
          TGF beta-1 Antibody in Western Blot (WB)
          TGF beta-1 Antibody in Western Blot (WB)
          TGF beta-1 Antibody in Western Blot (WB)
          TGF beta-1 Antibody in Immunocytochemistry (ICC/IF)
          TGF beta-1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          TGF beta-1 Antibody in Western Blot (WB)
          TGF beta-1 Antibody in Western Blot (WB)
          TGF beta-1 Antibody in Western Blot (WB)
          TGF beta-1 Antibody in Western Blot (WB)
          TGF beta-1 Antibody in Western Blot (WB)
          TGF beta-1 Antibody in Western Blot (WB)
          TGF beta-1 Antibody in Western Blot (WB)
          TGF beta-1 Antibody
          TGF beta-1 Antibody
          TGF beta-1 Antibody

          产品信息

          MA5-15065

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:1,000
          查看9篇已发表文章 9篇已发表文章

          免疫组化 (IHC)

          -
          查看1篇已发表文章 1篇已发表文章

          免疫细胞化学 (ICC/IF)

          -
          查看2篇已发表文章 2篇已发表文章
          产品规格

          种属反应

          Human

          已发表种属

          Human, Mouse, Rat

          宿主/亚型

          Rabbit / IgG

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          F.888.7

          抗原

          Synthetic peptide corresponding to a region in the carboxy terminus of TGF-ß1 protein
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          194 µg/mL

          纯化类型

          Affinity chromatography

          保存液

          0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

          内含物

          <0.02% sodium azide

          保存条件

          -20°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10984297

          产品详细信息

          It is not recommended to aliquot this antibody.

          靶标信息

          TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: latency-associated peptide; prepro-transforming growth factor beta-1; TGF b; TGF b 1; TGF beta; tgf beta 1; TGF beta-1; TGF beta-1 protein; tgf beta1; TGF β; TGF β 1; TGF β1; TGF-B1; TGF-beta 1 protein; TGF-beta-1; TGFβ1; Transforming growth factor; transforming growth factor b1; transforming growth factor beta; Transforming growth factor beta-1 proprotein; transforming growth factor β1

          查看更多 收起

          基因别名: CED; DPD1; LAP; TGFB; TGFB1; TGFbeta

          查看更多 收起

          UniProt ID: (Human) P01137

          查看更多 收起

          Entrez Gene ID: (Human) 7040

          查看更多 收起

          功能
          glycoprotein binding antigen binding type II transforming growth factor beta receptor binding cytokine activity transforming growth factor beta receptor binding protein binding growth factor activity enzyme binding type I transforming growth factor beta receptor binding type III transforming growth factor beta receptor binding protein homodimerization activity protein serine/threonine kinase activator activity protein heterodimerization activity protein N-terminus binding growth factor
          参与通路
          protein import into nucleus, translocation negative regulation of transcription from RNA polymerase II promoter MAPK cascade ureteric bud development response to hypoxia epithelial to mesenchymal transition negative regulation of protein phosphorylation positive regulation of protein phosphorylation regulation of sodium ion transport chondrocyte differentiation hematopoietic progenitor cell differentiation connective tissue replacement involved in inflammatory response wound healing adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains tolerance induction to self antigen platelet degranulation protein phosphorylation protein export from nucleus ATP biosynthetic process phosphate-containing compound metabolic process cellular calcium ion homeostasis inflammatory response cell cycle arrest mitotic cell cycle checkpoint epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway common-partner SMAD protein phosphorylation SMAD protein complex assembly SMAD protein import into nucleus Notch signaling pathway negative regulation of neuroblast proliferation salivary gland morphogenesis endoderm development female pregnancy aging negative regulation of DNA replication positive regulation of cell proliferation negative regulation of cell proliferation germ cell migration response to radiation response to wounding response to glucose defense response to fungus, incompatible interaction positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression negative regulation of extracellular matrix disassembly positive regulation of epithelial to mesenchymal transition macrophage derived foam cell differentiation positive regulation of fibroblast migration positive regulation of peptidyl-threonine phosphorylation positive regulation of pathway-restricted SMAD protein phosphorylation negative regulation of macrophage cytokine production oligodendrocyte development cell growth regulation of striated muscle tissue development cell migration regulation of transforming growth factor beta receptor signaling pathway evasion or tolerance of host defenses by virus negative regulation of cell-cell adhesion hyaluronan catabolic process negative regulation of ossification negative regulation of cell growth regulation of cell migration positive regulation of cell migration positive regulation of bone mineralization negative regulation of transforming growth factor beta receptor signaling pathway positive regulation of histone deacetylation organ regeneration positive regulation of protein complex assembly positive regulation of exit from mitosis lipopolysaccharide-mediated signaling pathway positive regulation of cellular protein metabolic process response to estradiol response to progesterone regulation of interleukin-23 production negative regulation of interleukin-17 production positive regulation of interleukin-17 production receptor catabolic process positive regulation of superoxide anion generation mononuclear cell proliferation positive regulation of collagen biosynthetic process positive regulation of peptidyl-serine phosphorylation response to vitamin D response to laminar fluid shear stress positive regulation of histone acetylation positive regulation of protein dephosphorylation negative regulation of T cell proliferation regulation of protein import into nucleus positive regulation of protein import into nucleus positive regulation of odontogenesis response to drug myelination regulation of apoptotic process myeloid dendritic cell differentiation T cell homeostasis positive regulation of apoptotic process positive regulation of vascular permeability positive regulation of MAP kinase activity protein kinase B signaling positive regulation of blood vessel endothelial cell migration negative regulation of blood vessel endothelial cell migration positive regulation of phosphatidylinositol 3-kinase activity ossification involved in bone remodeling regulatory T cell differentiation cell-cell junction organization positive regulation of regulatory T cell differentiation negative regulation of cell differentiation negative regulation of fat cell differentiation negative regulation of myoblast differentiation negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter active induction of host immune response by virus positive regulation of fibroblast proliferation positive regulation of isotype switching to IgA isotypes lymph node development digestive tract development negative regulation of skeletal muscle tissue development inner ear development positive regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation positive regulation of protein secretion positive regulation of peptidyl-tyrosine phosphorylation negative regulation of phagocytosis positive regulation of chemotaxis positive regulation of NF-kappaB transcription factor activity regulation of binding regulation of DNA binding positive regulation of smooth muscle cell differentiation negative regulation of release of sequestered calcium ion into cytosol positive regulation of cell division positive regulation of protein kinase B signaling regulation of blood vessel remodeling face morphogenesis frontal suture morphogenesis pathway-restricted SMAD protein phosphorylation regulation of SMAD protein import into nucleus positive regulation of SMAD protein import into nucleus SMAD protein signal transduction mammary gland branching involved in thelarche branch elongation involved in mammary gland duct branching regulation of branching involved in mammary gland duct morphogenesis negative regulation of gene silencing by miRNA regulation of cartilage development lens fiber cell differentiation response to cholesterol positive regulation of cell cycle arrest cellular response to organic cyclic compound cellular response to dexamethasone stimulus cellular response to transforming growth factor beta stimulus extracellular matrix assembly positive regulation of branching involved in ureteric bud morphogenesis extrinsic apoptotic signaling pathway negative regulation of hyaluronan biosynthetic process positive regulation of extracellular matrix assembly positive regulation of NAD+ ADP-ribosyltransferase activity positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of protein localization to plasma membrane negative regulation of production of miRNAs involved in gene silencing by miRNA positive regulation of receptor clustering regulation of epithelial to mesenchymal transition involved in endocardial cushion formation regulation of actin cytoskeleton reorganization positive regulation of transcription regulatory region DNA binding positive regulation of cardiac muscle cell differentiation
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