Invitrogen

TGN46 Polyclonal Antibody

5个已发表图片

10 篇参考文献

查看其他15个TGN46抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (8)
已发表图片 (5)

TGN46 Antibody in Immunocytochemistry (ICC/IF)

TGN46 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 13

TGN46 Antibody (PA5-23068) in ICC/IF

Immunofluorescence analysis of TGN46 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TGN46 Polyclonal Antibody (Product # PA5-23068) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # ... View More

TGN46 Antibody in Immunocytochemistry (ICC/IF)

TGN46 Antibody in Immunocytochemistry (ICC/IF)

TGN46 Antibody in Immunocytochemistry (ICC/IF)

TGN46 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TGN46 Antibody in Western Blot (WB)

TGN46 Antibody in Western Blot (WB)

TGN46 Antibody in Western Blot (WB)

TGN46 Antibody in Flow Cytometry (Flow)

TGN46 Antibody in Immunocytochemistry (ICC/IF)

TGN46 Antibody in Immunocytochemistry (ICC/IF)

TGN46 Antibody in Immunocytochemistry (ICC/IF)

TGN46 Antibody in Immunocytochemistry (ICC/IF)

TGN46 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

TGN46 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of TGN46 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TGN46 Polyclonal Antibody (Product # PA5-23068) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

TGN46 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of TGN46 in HeLa cells. Samples were incubated with TGN46 polyclonal antibody (Product # PA5-23068) using a dilution of 2 µg/mL for 60 minutes at room temperature followed by anti-rabbit Dylight 488 (Green) at a dilution of 1:1,000 dilution for 60 minutes. Cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective.

TGN46 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of TGN46 in A549 cells. Samples were incubated in TGN46 polyclonal antibody (Product # PA5-23068) followed by Alexa Fluor 488-conjugated Goat to rabbit IgG secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

TGN46 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of TGN46 in human renal cell carcinoma. Samples were incubated in TGN46 polyclonal antibody (Product # PA5-23068).

TGN46 Antibody in Western Blot (WB) — Western blot was performed using TGN46 Polyclonal Antibody (Product # PA5-23068) and a 39 kDa band corresponding to TGN46 was observed across cell lines and tissues tested along with an uncharacterized band (*) at ~80 kDa. Modified whole cell extracts (30 µg lysate) of HeLa (Lane 1), T98G (Lane 2), Mouse muscle (Lane 3), Rat muscle (Lane 4), Mouse skin (Lane 5) and Rat skin (Lane 6) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (PA5-23068, 1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

TGN46 Antibody in Western Blot (WB) — Western blot analysis of TGN46 in lysates of HeLa human cervical epithelial carcinoma parental cell line and TGN46 knockout (KO) HeLa cell line. Samples were incubated with TGN46 polyclonal antibody (Product # PA5-23068) using a dilution of 1:1,000 followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. PVDF membrane. Specific band was detected for TGN46 at approximately 100 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

TGN46 Antibody in Western Blot (WB) — Western blot analysis of TGN46 in extracts from HeLa cells. Samples were incubated in TGN46 polyclonal antibody (Product # PA5-23068) using a dilution of 1:100.

TGN46 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of TGN46 in U-937 cells. Samples were incubated with TGN46 polyclonal antibody (Product # PA5-23068) at a dilution of 1 µg/mL for 30 minutes at room temperature followed by rabbit IgG APC-conjugated secondary antibody. An intracellular stain was performed with primary antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin.

TGN46 Antibody in Immunocytochemistry (ICC/IF) — Figure 4. ATP6V0C is required for HCMV virion assembly compartment biogenesis. Fibroblast cells were transfected with ATP6V0C siRNA or negative control siRNA and at 72 h post-transfection were infected with AD169. At 144 hpi cells were fixed, permeabilised and stained for early endosomes or trans-Golgi vacuoles, (EEA1:green or TGN46:green), viral tegument protein (pp28:red) and nuclei (DAPI:blue). Images were acquired on a Zeiss LSM710 confocal microscope. Images presented here are maximum-intensity projections compiled from multiple 0.33 um slices through the z -axis.

TGN46 Antibody in Immunocytochemistry (ICC/IF) — Published figure using TGN46 polyclonal antibody (Product # PA5-23068) in Immunocytochemistry

TGN46 Antibody in Immunocytochemistry (ICC/IF) — Published figure using TGN46 polyclonal antibody (Product # PA5-23068) in Immunocytochemistry

TGN46 Antibody in Immunocytochemistry (ICC/IF) — Published figure using TGN46 polyclonal antibody (Product # PA5-23068) in Immunocytochemistry

TGN46 Antibody in Western Blot (WB) — Published figure using TGN46 polyclonal antibody (Product # PA5-23068) in Western Blot

Immunogen image

TGN46 Polyclonal Antibody

Immunogen Image TGN46 Polyclonal Antibody

产品信息

PA5-23068

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:400

-

免疫细胞化学 (ICC/IF)

1:500

查看7篇已发表文章 7篇已发表文章

流式细胞分析 (Flow)

Assay-Dependent

-

其他PubMed (Misc)

-

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Bovine, Human, Mouse, Non-human primate

已发表种属

Human, Non-human primate

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A genomic peptide made to an internal region of the human TGN46 protein (within residues 200-350).

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

靶标信息

IASPP is one of the most evolutionarily conserved inhibitors of p53 (TP53; MIM 191170), whereas ASPP1. and ASPP2. are activators of p53.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: TGN38B; Trans-Golgi network integral membrane protein 2

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基因别名: TGN38B; TGN41; TGN46; Tgoln2; Ttgn2

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UniProt ID: (Mouse) Q62314

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Entrez Gene ID: (Mouse) 22135

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membrane traffic protein