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TIMP1 Antibody (PA5-95475) in WB
Western blot analysis of TIMP1 in Lane 1: human HeLa whole cell lysates, Lane 2: rat ovary tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse ovary tissue lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with TIMP1 polyclonal antibody (Product # PA5-95475) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a ... View More
Product Details
PA5-95475
Applications
Tested Dilution
Publications
产品规格
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
Immunogen
E.coli-derived human TIMP1 recombinant protein (Position: C24-A207).
Conjugate
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_2807277
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
TIMP1 (metalloproteinase inhibitor 1) is a metalloproteinase inhibitor that functions by forms one-to-one complexes with target metallproteinases, such as collagenases, and irreversible inactivates them by binding to their catalytic zinc cofactor. TIMP1 acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP14, and MMP16. It also functions as a growth factor that regulates cell differenation, migration, and cell death. TIMP1 activates cellular signaling cascades via CD63 and ITGB1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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Bioinformatics
Protein Aliases: Collagenase inhibitor; Collagenase inhibitor 16C8 fibroblast; EPA; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; Metalloproteinase inhibitor; Metalloproteinase inhibitor 1; MMP inhibitor; RP1-230G1.3; TIMP 1; TIMP-1; tissue inhibitor of metallopeptidase 1; tissue inhibitor of metalloproteinase 1; tissue inhibitor of metalloproteinase-1; Tissue inhibitor of metalloproteinases 1; TPA-induced protein; TPA-S1
Gene Aliases: CLGI; EPA; EPO; HCI; TIMP; TIMP-1; TIMP1; TPA-S1
UniProt ID: (Human) P01033, (Rat) P30120, (Mouse) P12032
Entrez Gene ID: (Human) 7076, (Rat) 116510, (Mouse) 21857
Molecular Function:
protease inhibitor
cell activation
platelet degranulation
aging
positive regulation of cell proliferation
response to hormone
negative regulation of endopeptidase activity
extracellular matrix disassembly
response to cytokine
wound healing
negative regulation of apoptotic process
negative regulation of catalytic activity
response to peptide hormone
negative regulation of membrane protein ectodomain proteolysis
cartilage development
negative regulation of trophoblast cell migration
regulation of integrin-mediated signaling pathway
steroid biosynthetic process
response to organic substance
negative regulation of peptidase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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