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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • TIMP2 Antibodies

          Invitrogen

          TIMP2 Monoclonal Antibody (F27 P3 A4)

          高级验证
          1个已发表图片
          5 篇参考文献
          查看其他36个TIMP2抗体

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          说明书
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          问题与答案
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          说明书
          实验方案
          问题与答案
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          Cite TIMP2 Monoclonal Antibody (F27 P3 A4)

          • 抗体检测数据 (7)
          • 已发表图片 (1)
          • 高级验证 (1)
          TIMP2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          TIMP2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 9

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          TIMP2 Antibody (MA1-774) in ICC/IF

          Immunofluorescence analysis of TIMP2 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TIMP2 (F27 P3 A4) Mouse Monoclonal Antibody (Product # MA1-774) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
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          查看产品

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          TIMP2 Antibody in Immunocytochemistry (ICC/IF)
          TIMP2 Antibody in Immunocytochemistry (ICC/IF)
          TIMP2 Antibody in Immunocytochemistry (ICC/IF)
          TIMP2 Antibody in Immunocytochemistry (ICC/IF)
          TIMP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          TIMP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          TIMP2 Antibody in Western Blot (WB)
          TIMP2 Antibody in Western Blot (WB)
          TIMP2 Antibody

          产品信息

          MA1-774

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:500
          查看4篇已发表文章 4篇已发表文章

          免疫组化 (IHC)

          -
          查看1篇已发表文章 1篇已发表文章

          免疫组化(石蜡) (IHC (P))

          1:50-1:500
          -

          免疫细胞化学 (ICC/IF)

          1-2 µg/mL
          -
          产品规格

          种属反应

          Human, Mouse

          已发表种属

          Human, Mouse, Plant

          宿主/亚型

          Mouse / IgG2a, kappa

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          F27 P3 A4

          抗原

          Synthetic peptide corresponding to residues D(56) S G N D I Y G N P I(66) of mouse TIMP-2.
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          1 mg/mL

          纯化类型

          Protein A

          保存液

          PBS with 1mg/mL BSA

          内含物

          0.05% sodium azide

          保存条件

          -20°C, Avoid Freeze/Thaw Cycles

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_889483

          产品详细信息

          MA1-774 contains 100 µg of in vitro produced, protein A purified antibody (1 mg/mL) in PBS containing 1 mg/mL BSA and 0.05% sodium azide.

          MA1-774 detects TIMP-2 from mouse samples.

          MA1-774 has been successfully used in Western blot, IF and immunohistochemistry procedures. By Western blot, MA1-774 detects a ~18-24 kDa band representing TIMP-2 from mouse platelets. It is recommended to block with 3% BSA.

          The MA1-774 immunogen is a synthetic peptide corresponding to residues D(56) S G N D I Y G N P I(66) of mouse TIMP-2. This sequence is 100% conserved in human, rat, rabbit, chicken, and bovine TIMP-2

          靶标信息

          TIMP-1 and TIMP-2 are inhibitory enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-2 shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: CSC-21K; Metalloproteinase inhibitor 2; testicular secretory protein Li 57; TIMP 2; TIMP-2; Tissue inhibitor of metalloproteinases 2

          查看更多 收起

          基因别名: CSC-21K; D11Bwg1104e; DDC8; Timp-2; TIMP2

          查看更多 收起

          UniProt ID: (Human) P16035

          查看更多 收起

          Entrez Gene ID: (Human) 7077, (Mouse) 21858

          查看更多 收起

          功能
          protease binding integrin binding protein binding metalloendopeptidase inhibitor activity metal ion binding enzyme inhibitor activity enzyme activator activity peptidase inhibitor activity protease inhibitor
          参与通路
          central nervous system development aging negative regulation of cell proliferation response to hormone negative regulation of endopeptidase activity extracellular matrix disassembly regulation of Rap protein signal transduction response to cytokine response to drug positive regulation of MAPK cascade positive regulation of neuron differentiation positive regulation of adenylate cyclase activity negative regulation of mitotic cell cycle negative regulation of Ras protein signal transduction negative regulation of membrane protein ectodomain proteolysis negative regulation of peptidase activity regulation of cAMP metabolic process positive regulation of catalytic activity negative regulation of catalytic activity regulation of MAPK cascade regulation of neuron differentiation negative regulation of proteolysis cellular response to organic substance
          It has to be done as per old AB suggested Products section.
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          性能保障

          只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符,您即可享受免费退换货服务。*

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