Invitrogen

TIMP2 Monoclonal Antibody (F27 P3 A4)

TIMP2 Antibody in Immunocytochemistry (ICC/IF)

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TIMP2 Antibody (MA1-774) in ICC/IF

Immunofluorescence analysis of TIMP2 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TIMP2 (F27 P3 A4) Mouse Monoclonal Antibody (Product # MA1-774) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) ... View More

TIMP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TIMP2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lysates tested owing to their inherent genetic constitution. Relative expression of TIMP2 was observed in HT-1080 as compared to Daudi using Anti-TIMP2 Monoclonal Antibody (F27 P3 A4) (Product # MA1-774) in Western Blot. {RE}

产品信息

MA1-774

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:500

免疫细胞化学 (ICC/IF)

1-2 µg/mL

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Plant

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

F27 P3 A4

抗原

Synthetic peptide corresponding to residues D(56) S G N D I Y G N P I(66) of mouse TIMP-2.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_889483

产品详细信息

MA1-774 contains 100 µg of in vitro produced, protein A purified antibody (1 mg/mL) in PBS containing 1 mg/mL BSA and 0.05% sodium azide.

MA1-774 detects TIMP-2 from mouse samples.

MA1-774 has been successfully used in Western blot, IF and immunohistochemistry procedures. By Western blot, MA1-774 detects a ~18-24 kDa band representing TIMP-2 from mouse platelets. It is recommended to block with 3% BSA.

The MA1-774 immunogen is a synthetic peptide corresponding to residues D(56) S G N D I Y G N P I(66) of mouse TIMP-2. This sequence is 100% conserved in human, rat, rabbit, chicken, and bovine TIMP-2

靶标信息

TIMP-1 and TIMP-2 are inhibitory enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-2 shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Therapeutic Benefit of Galectin-1: Beyond Membrane Repair, a Multifaceted Approach to LGMD2B.

Cells

Vallecillo-Zúniga ML,Poulson PD,Luddington JS,Arnold CJ,Rathgeber M,Kartchner BC,Hayes S,Gill H,Valdoz JC,Spallino JL,Garfield S,Dodson EL,Arthur CM,Stowell SR,Van Ry PM

MA1-774 was used in Western Blot to demonstrate that the reduced dimeric form of Gal-1 is responsible for inducing improvements to muscle membrane repair, while the monomeric form is responsible for changes in inflammation.

Wed Nov 17 00:00:00 UTC 2021

Therapeutic Benefit of Galectin-1: Beyond Membrane Repair, a Multifaceted Approach to LGMD2B.

Cells

Vallecillo-Zúniga ML,Poulson PD,Luddington JS,Arnold CJ,Rathgeber M,Kartchner BC,Hayes S,Gill H,Valdoz JC,Spallino JL,Garfield S,Dodson EL,Arthur CM,Stowell SR,Van Ry PM

Wed Nov 17 00:00:00 UTC 2021

Protective Effects of Kuding Tea (Ilex kudingcha C. J. Tseng) Polyphenols on UVB-Induced Skin Aging in SKH1 Hairless Mice.

Molecules (Basel, Switzerland)

Yi R,Zhang J,Sun P,Qian Y,Zhao X

MA1-774 was used in Western Blotting to study the protective effects of Kuding tea polyphenols on ultraviolet B-induced skin injury of SKH1 hairless mice.

Wed Mar 13 00:00:00 UTC 2019

A Genetic Screen Identifies PRP18a, a Putative Second Step Splicing Factor Important for Alternative Splicing and a Normal Phenotype in Arabidopsis thaliana.

G3 (Bethesda, Md.)

Kanno T,Lin WD,Chang CL,Matzke M,Matzke AJM

MA1-774 was used in Western Blotting to identify a putative second step splicing factor, important for alternative splicing and a normal phenotype in Arabidopsis thaliana.

Wed Mar 28 00:00:00 UTC 2018

CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes.

Cancer cell

Xia L,Huang W,Bellani M,Seidman MM,Wu K,Fan D,Nie Y,Cai Y,Zhang YW,Yu LR,Li H,Zahnow CA,Xie W,Chiu Yen RW,Rassool FV,Baylin SB

MA1-774 was used in Immunohistochemistry to identify that CHD4 knockdown activates silenced tumour suppressor genes, revealing their role for blunting colorectal cancer cell proliferation, invasion, and metastases.