Invitrogen

TLR2 Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

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说明书

实验方案

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TLR2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of TLR2 was performed using THP-1 cells and THP-1 treated with PMA (10 ng/mL, 20 hours) and LPS (20 ng/mL, 6 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TLR2 Polyclonal Antibody (Product # PA1-21611) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11078) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green) in THP-1 treated cells. Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image of THP-1 treated cells, which shows higher expression for TLR2 protein showing localization in lipid rafts in membrane. Panel e represents the merged image of THP-1 cells, that shows lower expression for TLR2 protein. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

TLR2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of TLR2/CD282 in human spleen tissue using a TLR2/CD282 polyclonal antibody (Product # PA1-21611).

TLR2 Antibody in Western Blot (WB) — Western blot was performed using Anti-TLR2 Polyclonal Antibody (Product # PA1-21611) and a 90 kDa band corresponding to TLR2 was observed in human THP-1 cell line tested and not in mouse Raw 264.7 cell line. Membrane enriched extracts (30 µg lysate) of THP-1 (Lane 1), THP-1 treated with 10 ng/mL PMA for 20 hours and 20 ng/mL LPS for 6 hours (Lane 2), Raw 264.7 (Lane 3) and Raw 264.7 treated with 1 µg/mL LPS for 6 hours (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

TLR2 Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis of TLR2 using TLR2 Goat Polyclonal Antibody (Product # PA1-21611) shows increased expression of TLR2 protein in THP-1 cell line upon treatment with PMA and LPS in membrane lipid rafts. {TM}

产品信息

PA1-21611

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:1,000	-
免疫组化 (IHC)	Assay-dependent	-
免疫组化（石蜡） (IHC (P))	1:250	-
免疫细胞化学 (ICC/IF)	1:100-1:500	-
酶联免疫吸附实验 (ELISA)	1:37,500	-

产品规格
种属反应	Human
宿主/亚型	Goat / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthetic peptide: CLEIDASDLQSYEPKSLKSIQNVSHLI, corresponding to amino acids 179-204 of Human TLR2.
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS with 1mg/mL BSA
内含物	0.1% sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_561722

产品详细信息

Recommended positive controls: Human spleen sections or human peripheral blood.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CD282; TLR-2; Toll-like receptor 2; Toll/interleukin-1 receptor-like protein 4

基因别名: CD282; TIL4; TLR2

UniProt ID: (Human) O60603

Entrez Gene ID: (Human) 7097

功能

参与通路

lipopolysaccharide binding lipopolysaccharide receptor activity receptor activity transmembrane signaling receptor activity protein binding signaling pattern recognition receptor activity Toll-like receptor binding triacyl lipopeptide binding peptidoglycan binding protein heterodimerization activity

TLR2 Polyclonal Antibody

产品信息

PA1-21611

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

酶联免疫吸附实验 (ELISA)

种属反应

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

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