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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • TLR2 Antibodies

          Invitrogen

          TLR2 Polyclonal Antibody

          Advanced Verification

          This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

          View all (44) TLR2 antibodies
          Datasheet
          Protocols
          Tech Support

          Cite TLR2 Polyclonal Antibody

          Datasheet
          Protocols
          Tech Support
          • Antibody Testing Data (3)
          • Advanced Verification (2)
          TLR2 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          TLR2 Antibody (PA1-21611) in WB

          Western blot was performed using Anti-TLR2 Polyclonal Antibody (Product # PA1-21611) and a 90 kDa band corresponding to TLR2 was observed in human THP-1 cell line tested and not in mouse Raw 264.7 cell line. Membrane enriched extracts (30 µg lysate) of THP-1 (Lane 1), THP-1 treated with 10 ng/mL PMA for 20 hours and 20 ng/mL LPS for 6 hours (Lane 2), Raw 264.7 (Lane 3) and Raw 264.7 treated with 1 µg/mL LPS for 6 hours (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins wer... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Product Details

          PA1-21611

          Applications
          Tested Dilution
          Publications

          免疫印迹 (WB)

          1:1,000
          -

          免疫组化(石蜡) (IHC (P))

          1:250
          -

          免疫细胞化学 (ICC/IF)

          1:100-1:500
          -

          酶联免疫吸附实验 (ELISA)

          1:37,500
          -
          产品规格

          Species Reactivity

          Human

          Host/Isotype

          Goat / IgG

          分类

          Polyclonal

          类型

          Antibody

          Immunogen

          Synthetic peptide: CLEIDASDLQSYEPKSLKSIQNVSHLI, corresponding to amino acids 179-204 of Human TLR2.
          View immunogen

          Conjugate

          Unconjugated

          形式

          Liquid

          浓度

          1 mg/mL

          纯化类型

          Antigen affinity chromatography

          保存液

          PBS with 1mg/mL BSA

          内含物

          0.1% sodium azide

          保存条件

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          RRID

          AB_561722

          Product Specific Information

          Recommended positive controls: Human spleen sections or human peripheral blood.

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          References (0)

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          Let us know so we can reference it here.
          Cite this product

          Bioinformatics

          Protein Aliases: CD282; TLR-2; Toll-like receptor 2; Toll/interleukin-1 receptor-like protein 4

          View more View less

          Gene Aliases: CD282; TIL4; TLR2

          View more View less

          UniProt ID: (Human) O60603

          View more View less

          Entrez Gene ID: (Human) 7097

          View more View less

          Function(s)
          cytokine secretion involved in immune response MyD88-dependent toll-like receptor signaling pathway apoptotic process inflammatory response immune response signal transduction I-kappaB phosphorylation positive regulation of gene expression positive regulation of Wnt signaling pathway lipopolysaccharide-mediated signaling pathway interleukin-10 production positive regulation of chemokine production positive regulation of interferon-beta production positive regulation of interleukin-12 production positive regulation of interleukin-18 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production positive regulation of toll-like receptor signaling pathway toll-like receptor 2 signaling pathway toll-like receptor TLR1:TLR2 signaling pathway toll-like receptor TLR6:TLR2 signaling pathway positive regulation of NF-kappaB import into nucleus detection of triacyl bacterial lipopeptide detection of diacyl bacterial lipopeptide innate immune response positive regulation of transcription from RNA polymerase II promoter regulation of cytokine secretion positive regulation of inflammatory response defense response to Gram-positive bacterium positive regulation of NF-kappaB transcription factor activity positive regulation of nitric-oxide synthase biosynthetic process cellular response to bacterial lipopeptide cellular response to lipoteichoic acid cellular response to diacyl bacterial lipopeptide cellular response to triacyl bacterial lipopeptide
          Process(es)
          lipopolysaccharide binding lipopolysaccharide receptor activity receptor activity transmembrane signaling receptor activity protein binding signaling pattern recognition receptor activity Toll-like receptor binding triacyl lipopeptide binding peptidoglycan binding protein heterodimerization activity
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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