Invitrogen
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
应用 | 建议稀释比 | 已发表文章 |
---|---|---|
免疫印迹 (WB) |
2-5 µg/mL | 查看2篇已发表文章 2篇已发表文章 |
免疫组化(石蜡) (IHC (P)) |
5 µg/mL | 查看1篇已发表文章 1篇已发表文章 |
免疫细胞化学 (ICC/IF) |
2-5 µg/mL | - |
流式细胞分析 (Flow) |
20 µg x 10^6 cells | - |
产品规格 | |
---|---|
种属反应 |
Human, Mouse, Rhesus monkey, Rat |
已发表种属 |
Mouse |
宿主/亚型 |
Rabbit / IgG |
分类 |
Polyclonal |
类型 |
Antibody |
抗原 |
Synthetic peptide (aa 39-56) of TLR4/CD284 protein. |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
1 mg/mL |
纯化类型 |
Protein G |
保存液 |
PBS |
内含物 |
0.05% sodium azide |
保存条件 |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2540651 |
Suggested positive control: recombinant protein, RAW, Daudi.
TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. Mammalian cells respond to LPS by activating TLR4. TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. Mutations in the TLR4 gene have been associated with differences in LPS responsiveness. Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain. TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: CD284; homolog of Drosophila toll; hToll; lipopolysaccharide response; TLR4; Toll-like receptor 4; toll/interleukin-l receptor (TIR) domain; Toll4
基因别名: ARMD10; CD284; EGK_07381; hToll; Lps; Ly87; Ran/M1; Rasl2-8; TLR-4; TLR4; TOLL
UniProt ID: (Human) O00206, (Mouse) Q9QUK6, (Rat) Q9QX05
Entrez Gene ID: (Rhesus monkey) 574360, (Human) 7099, (Mouse) 21898, (Rat) 29260