Invitrogen

TLR4 Recombinant Superclonal™ Antibody (20HCLC)

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TLR4 Recombinant Superclonal™ Antibody (20HCLC)

产品信息

710185

应用
建议稀释比
已发表文章

免疫印迹 (WB)

2-5 µg/mL
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免疫组化 (IHC)

-
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免疫组化（石蜡） (IHC (P))

1:20
-

免疫细胞化学 (ICC/IF)

1-3 µg/mL
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产品规格

种属反应

Human, Mouse

已发表种属

C. elegans, Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Superclonal

类型

Antibody

克隆号

20HCLC

抗原

Peptide corresponding to amino acids 816-830 of human Toll-like receptor 4
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2532610

产品详细信息

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

靶标信息

TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. Mammalian cells respond to LPS by activating TLR4. TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. Mutations in the TLR4 gene have been associated with differences in LPS responsiveness. Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain. TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: CD284; homolog of Drosophila toll; hToll; lipopolysaccharide response; Toll-like receptor 4

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基因别名: ARMD10; CD284; Lps; Ly87; Ran/M1; Rasl2-8; TLR-4; TLR4; TOLL

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UniProt ID: (Human) D1CS53, (Mouse) Q9QUK6

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Entrez Gene ID: (Human) 7099, (Mouse) 21898

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功能
lipopolysaccharide binding lipopolysaccharide receptor activity receptor activity transmembrane signaling receptor activity protein binding phosphatidylinositol 3-kinase binding
参与通路
activation of MAPK activity microglial cell activation activation of innate immune response toll-like receptor signaling pathway B cell proliferation involved in immune response immune system process nitric oxide production involved in inflammatory response regulation of dendritic cell cytokine production MyD88-dependent toll-like receptor signaling pathway innate immune response-activating signal transduction leukotriene metabolic process inflammatory response response to oxidative stress signal transduction activation of NF-kappaB-inducing kinase activity I-kappaB phosphorylation response to bacterium positive regulation of platelet activation positive regulation of B cell proliferation lipopolysaccharide-mediated signaling pathway response to lipopolysaccharide detection of lipopolysaccharide interferon-gamma production negative regulation of interferon-gamma production negative regulation of interleukin-17 production negative regulation of interleukin-23 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production positive regulation of chemokine production positive regulation of interferon-alpha production positive regulation of interferon-beta production positive regulation of interferon-gamma production positive regulation of interleukin-1 production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production positive regulation of stress-activated MAPK cascade toll-like receptor 4 signaling pathway macrophage activation positive regulation of NF-kappaB import into nucleus positive regulation of tumor necrosis factor biosynthetic process positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of DNA binding positive regulation of interleukin-12 biosynthetic process innate immune response positive regulation of MHC class II biosynthetic process positive regulation of interferon-beta biosynthetic process positive regulation of interleukin-1 biosynthetic process positive regulation of interleukin-13 biosynthetic process positive regulation of interleukin-6 biosynthetic process positive regulation of interleukin-8 biosynthetic process positive regulation of nitric oxide biosynthetic process response to ethanol mast cell activation positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade positive regulation of lymphocyte proliferation regulation of cytokine secretion regulation of inflammatory response positive regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity positive regulation of nitric-oxide synthase biosynthetic process regulation of sensory perception of pain intestinal epithelial structure maintenance positive regulation of macrophage cytokine production negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade positive regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway response to fatty acid cellular response to lipopolysaccharide cellular response to lipoteichoic acid MyD88-independent toll-like receptor signaling pathway immune response I-kappaB kinase/NF-kappaB signaling positive regulation of gene expression astrocyte development detection of fungus negative regulation of MyD88-independent toll-like receptor signaling pathway TRIF-dependent toll-like receptor signaling pathway T-helper 1 type immune response defense response to bacterium negative regulation of osteoclast differentiation interleukin-1 beta secretion necroptotic process cellular response to mechanical stimulus apoptotic signaling pathway positive regulation of NLRP3 inflammasome complex assembly
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