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产品信息
PETNFAI
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | 查看1篇已发表文章 1篇已发表文章 |
| 产品规格 | |
|---|---|
种属反应 |
Horse |
已发表种属 |
Bovine |
宿主/亚型 |
Rabbit / IgG |
分类 |
Polyclonal |
类型 |
Antibody |
抗原 |
Recombinant Equine TNF alpha |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
浓度 |
1 mg/mL |
纯化类型 |
Protein G |
保存液 |
PBS |
内含物 |
no preservative |
保存条件 |
4° C |
RRID |
AB_417106 |
产品详细信息
PETNFAI targets TNF Alpha in ELISA, and WB applications and shows reactivity with Equine samples.
The PETNFAI immunogen is recombinant Equine TNF alpha.
PETNFAI detects TNF Alpha which has a predicted molecular weight of approximately 25 kDa.
The PETNFAI equine TNFa polyclonal antibody has successfully been paired as the coating antibody in a sandwich ELISA with biotinylated detection antibody PETNFABI. Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.
Reconstitute in 1,000 µL PBS to create a stock of 1 mg/mL.
靶标信息
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: C-domain 1; C-domain 2; Cachectin; DADB-70P7.1; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; TNF alpha; TNF superfamily; TNF α; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand 1F; Tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha
基因别名: TNF; TNF-a; TNF-alpha; TNFA; TNFSF2; TNLG1F
UniProt ID: (Horse) P29553
Entrez Gene ID: (Horse) 100033834
