Invitrogen

TNF alpha Monoclonal Antibody (MAb11), eFluor™ 450, eBioscience™

TNF alpha Antibody in Flow Cytometry (Flow)

图: 1 / 30

TNF alpha Antibody (48-7349-42) in Flow

Intracellular staining of human peripheral blood cells unstimulated (left) or stimulated for 5 hours with PMA/ionomycin and Brefeldin A (right) with Anti-Human CD4 FITC (Product # 11-0048-42) and Anti-Human TNF alpha eFluor® 450. Cells in the lymphocyte gate were used for analysis.

TNF alpha Antibody in Immunohistochemistry (IHC)

TNF alpha Antibody in Flow Cytometry, Neutralization (Flow, Neu)

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产品信息

48-7349-42

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

5 µL (0.5 µg)/test

查看46篇已发表文章 46篇已发表文章

中和实验 (Neu)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse, Rhesus monkey

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

MAb11

偶联物

eFluor™ 450 eFluor™ 450 eFluor™ 450

激发/发射光谱

405/445 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2043889

产品详细信息

Description: The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TN alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.

Applications Reported: This MAb11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MAb11 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Antigen-Specific Intraocular Cytokine Responses Distinguish Ocular Tuberculosis From Undifferentiated Uveitis in Tuberculosis-Immunoreactive Patients.

American journal of ophthalmology

Alam K,Sharma G,Forrester JV,Basu S

48-7349-42 was used in Flow cytometry/Cell sorting to show that the intraocular polyfunctional cytokine response is stronger in undifferentiated TST+ uveitis than in OTB patients, likely representing an exaggerated anti-TB immune response rather than active infection.

Wed Feb 01 00:00:00 UTC 2023

The effect of metformin on senescence of T lymphocytes.

Immunity & ageing : I & A

Yang J,Liu HC,Zhang JQ,Zou JY,Zhang X,Chen WM,Gu Y,Hong H

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Tue Dec 12 00:00:00 UTC 2023

Dynamics of humoral and cellular response to three doses of anti-SARS-CoV-2 BNT162b2 vaccine in patients with hematological malignancies and older subjects.

Frontiers in immunology

Laquintana V,Mottini C,Marchesi F,Marcozzi B,Terrenato I,Sperandio E,de Latouliere L,Carrieri F,Pimpinelli F,Pontone M,Pellini R,Campo F,Conti L,Accetta C,Mandoj C,Petrone F,Di Bella O,Vujovic B,Morrone A,Compagnone M,Principato E,Pinto E,Papa E,Falcucci P,La Malfa A,Pallocca M,De Marco F,Piaggio G,Ciliberto G,Mengarelli A,di Martino S

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Wed Feb 14 00:00:00 UTC 2024

R274X-mutated Phf6 increased the self-renewal and skewed T cell differentiation of hematopoietic stem cells.

iScience

Lan Y,Yuan S,Guo T,Hou S,Zhao F,Yang W,Cao Y,Chu Y,Jiang E,Yuan W,Wang X

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Fri Jun 16 00:00:00 UTC 2023

An epitope encoded by uORF of RNF10 elicits a therapeutic anti-tumor immune response.

Molecular therapy oncolytics

Zeng L,Zheng W,Zhang J,Wang J,Ji Q,Wu X,Meng Y,Zhu X

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Tue Dec 19 00:00:00 UTC 2023

Targeted multi-omic analysis of human skin tissue identifies alterations of conventional and unconventional T cells associated with burn injury.

eLife

Labuz DR,Lewis G,Fleming ID,Thompson CM,Zhai Y,Firpo MA,Leung DT

48-7349-42 was used in Flow cytometry/Cell sorting to suggest that T cells in burn tissue have a pro-inflammatory rather than a homeostatic tissue-resident phenotype, and that unconventional T cells have a higher cytotoxic capacity.

Wed Feb 15 00:00:00 UTC 2023

Immunological memory to common cold coronaviruses assessed longitudinally over a three-year period pre-COVID19 pandemic.

Cell host & microbe

Yu ED,Narowski TM,Wang E,Garrigan E,Mateus J,Frazier A,Weiskopf D,Grifoni A,Premkumar L,da Silva Antunes R,Sette A

48-7349-42 was used in Flow cytometry/Cell sorting to understand the immune response to endemic coronaviruses and suggest that steady and sustainedcommon cold coronaviruses responses are likely from a stable pool of memory CD4+ T cells due to repeated earlier exposures and possibly occasional reinfections.

Wed Sep 14 00:00:00 UTC 2022

NVX-CoV2373 vaccination induces functional SARS-CoV-2-specific CD4+ and CD8+ T cell responses.

The Journal of clinical investigation

Rydyznski Moderbacher C,Kim C,Mateus J,Plested J,Zhu M,Cloney-Clark S,Weiskopf D,Sette A,Fries L,Glenn G,Crotty S

48-7349-42 was used in Flow cytometry/Cell sorting to demonstrate that vaccinated subjects made CD4+ T cell responses after one and two doses of NVX-CoV2373, and a subset of individuals made CD8+ T cell responses.

Mon Oct 03 00:00:00 UTC 2022

LINC01123 promotes immune escape by sponging miR-214-3p to regulate B7-H3 in head and neck squamous-cell carcinoma.

Cell death & disease

Li H,Yang Z,Yang X,Zhang F,Wang J,Wu Z,Wanyan C,Meng Q,Gao W,Yang X,Wei J

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Thu Feb 03 00:00:00 UTC 2022

PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.

Nature communications

Li Q,Zhang L,You W,Xu J,Dai J,Hua D,Zhang R,Yao F,Zhou S,Huang W,Dai Y,Zhang Y,Baheti T,Qian X,Pu L,Xu J,Xia Y,Zhang C,Tang J,Wang X

48-7349-42 was used in Flow cytometry/Cell sorting to demonstrate that the PRDM1-USP22-SPI1 axis regulates PD-L1 levels, resulting in infiltrated CD8+ T cell exhaustion.

Mon Dec 12 00:00:00 UTC 2022

PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.

Nature communications

Li Q,Zhang L,You W,Xu J,Dai J,Hua D,Zhang R,Yao F,Zhou S,Huang W,Dai Y,Zhang Y,Baheti T,Qian X,Pu L,Xu J,Xia Y,Zhang C,Tang J,Wang X

48-7349-42 was used in Flow cytometry/Cell sorting to demonstrate that the PRDM1-USP22-SPI1 axis regulates PD-L1 levels, resulting in infiltrated CD8+ T cell exhaustion.

Mon Dec 12 00:00:00 UTC 2022

PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.

Nature communications

Li Q,Zhang L,You W,Xu J,Dai J,Hua D,Zhang R,Yao F,Zhou S,Huang W,Dai Y,Zhang Y,Baheti T,Qian X,Pu L,Xu J,Xia Y,Zhang C,Tang J,Wang X

48-7349-42 was used in Flow cytometry/Cell sorting to demonstrate that the PRDM1-USP22-SPI1 axis regulates PD-L1 levels, resulting in infiltrated CD8+ T cell exhaustion.

Mon Dec 12 00:00:00 UTC 2022

Enhanced antibody responses in fully vaccinated individuals against pan-SARS-CoV-2 variants following Omicron breakthrough infection.

Cell reports. Medicine

Jeong HW,Kim SM,Jung MK,Noh JY,Yoo JS,Kim EH,Kim YI,Yu K,Jang SG,Gil J,Casel MA,Rare R,Choi JH,Kim HS,Kim JH,Um J,Kim C,Kim Y,Chin BS,Jung S,Choi JY,Song KH,Kim YD,Park JS,Song JY,Shin EC,Choi YK

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Tue Oct 18 00:00:00 UTC 2022

SARS-CoV-2 vaccination induces immunological T cell memory able to cross-recognize variants from Alpha to Omicron.

Cell

Tarke A,Coelho CH,Zhang Z,Dan JM,Yu ED,Methot N,Bloom NI,Goodwin B,Phillips E,Mallal S,Sidney J,Filaci G,Weiskopf D,da Silva Antunes R,Crotty S,Grifoni A,Sette A

48-7349-42 was used in Flow Cytometry to address whether T cell responses induced by different vaccine platforms (mRNA-1273, BNT162b2, Ad26.COV2.S, and NVX-CoV2373) cross-recognize early SARS-CoV-2 variants.

Thu Mar 03 00:00:00 UTC 2022

Humoral and cellular immune memory to four COVID-19 vaccines.

Cell

Zhang Z,Mateus J,Coelho CH,Dan JM,Moderbacher CR,Gálvez RI,Cortes FH,Grifoni A,Tarke A,Chang J,Escarrega EA,Kim C,Goodwin B,Bloom NI,Frazier A,Weiskopf D,Sette A,Crotty S

48-7349-42 was used in flow cytometry to investigate SARS-CoV-2-spike-specific immune responses to Moderna mRNA-1273, Pfizer/BioNTech BNT162b2, Janssen Ad26.COV2.S, and Novavax NVX-CoV2373.

Thu Jul 07 00:00:00 UTC 2022

Dissecting the role of CSF2RB expression in human regulatory T cells.

Frontiers in immunology

Côrte-Real BF,Arroyo Hornero R,Dyczko A,Hamad I,Kleinewietfeld M

48-7349-42 was used in Flow Cytometry to identify Treg subpopulations from pools of activated CD4+ T cells and demonstrate the overexpression of CSF2RB in Tregs of patients with autoimmune diseases like MS and SLE, thereby indicating that CSF2RB expression in Tregs could serve as a potential novel biomarker for disease.

Tue Dec 20 00:00:00 UTC 2022

Early and Polyantigenic CD4 T Cell Responses Correlate with Mild Disease in Acute COVID-19 Donors.

International journal of molecular sciences

Tarke A,Potesta M,Varchetta S,Fenoglio D,Iannetta M,Sarmati L,Mele D,Dentone C,Bassetti M,Montesano C,Mondelli MU,Filaci G,Grifoni A,Sette A

48-7349-42 was used in Flow cytometry/Cell sorting to highlight that both the magnitude and breadth of early SARS-CoV-2-specific CD4+ T cell responses correlated with milder disease outcomes in acute COVID-19 patients.

Tue Jun 28 00:00:00 UTC 2022

Defining antigen targets to dissect vaccinia virus and monkeypox virus-specific T cell responses in humans.

Cell host & microbe

Grifoni A,Zhang Y,Tarke A,Sidney J,Rubiro P,Reina-Campos M,Filaci G,Dan JM,Scheuermann RH,Sette A

48-7349-42 was used in Flow cytometry/Cell sorting to predict potential monkeypox virus (MPXV) targets recognized by CD4+ and CD8+ T cell responses based on vaccinia virus (VACV) epitopes, revealing a high degree of conservation between VACV and MPXV epitopes, which allowed the design of peptide pools to detect VACV-specific T cell responses and cross-reactive T cells in vaccinated individuals.

Wed Dec 14 00:00:00 UTC 2022

Baricitinib treatment resolves lower-airway macrophage inflammation and neutrophil recruitment in SARS-CoV-2-infected rhesus macaques.

Cell

Hoang TN,Pino M,Boddapati AK,Viox EG,Starke CE,Upadhyay AA,Gumber S,Nekorchuk M,Busman-Sahay K,Strongin Z,Harper JL,Tharp GK,Pellegrini KL,Kirejczyk S,Zandi K,Tao S,Horton TR,Beagle EN,Mahar EA,Lee MYH,Cohen J,Jean SM,Wood JS,Connor-Stroud F,Stammen RL,Delmas OM,Wang S,Cooney KA,Sayegh MN,Wang L,Filev PD,Weiskopf D,Silvestri G,Waggoner J,Piantadosi A,Kasturi SP,Al-Shakhshir H,Ribeiro SP,Sekaly RP,Levit RD,Estes JD,Vanderford TH,Schinazi RF,Bosinger SE,Paiardini M

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Thu Jan 21 00:00:00 UTC 2021

Membrane lymphotoxin-α₂β is a novel tumor necrosis factor (TNF) receptor 2 (TNFR2) agonist.

Cell death & disease

Kucka K,Lang I,Zhang T,Siegmund D,Medler J,Wajant H

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Tue Apr 06 00:00:00 UTC 2021

NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca²⁺ entry in T cells.

Cell reports

Wu B,Woo JS,Vila P,Jew M,Leung J,Sun Z,Srikanth S,Gwack Y

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Tue Aug 24 00:00:00 UTC 2021

Development of an Inflammatory CD14⁺ Dendritic Cell Subset in Humanized Mice.

Frontiers in immunology

Iwabuchi R,Ide K,Terahara K,Wagatsuma R,Iwaki R,Matsunaga H,Tsunetsugu-Yokota Y,Takeyama H,Takahashi Y

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Mon Sep 27 00:00:00 UTC 2021

Cellular and humoral immune responses following SARS-CoV-2 mRNA vaccination in patients with multiple sclerosis on anti-CD20 therapy.

Nature medicine

Apostolidis SA,Kakara M,Painter MM,Goel RR,Mathew D,Lenzi K,Rezk A,Patterson KR,Espinoza DA,Kadri JC,Markowitz DM,E Markowitz C,Mexhitaj I,Jacobs D,Babb A,Betts MR,Prak ETL,Weiskopf D,Grifoni A,Lundgreen KA,Gouma S,Sette A,Bates P,Hensley SE,Greenplate AR,Wherry EJ,Li R,Bar-Or A

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Mon Nov 01 00:00:00 UTC 2021

Rejuvenating Effector/Exhausted CAR T Cells to Stem Cell Memory-Like CAR T Cells By Resting Them in the Presence of CXCL12 and the NOTCH Ligand.

Cancer research communications

Ando M,Kondo T,Tomisato W,Ito M,Shichino S,Srirat T,Mise-Omata S,Nakagawara K,Yoshimura A

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Fri Oct 01 00:00:00 UTC 2021

Inhibition of Cholesterol Esterification Enzyme Enhances the Potency of Human Chimeric Antigen Receptor T Cells against Pancreatic Carcinoma.

Molecular therapy oncolytics

Zhao L,Liu Y,Zhao F,Jin Y,Feng J,Geng R,Sun J,Kang L,Yu L,Wei Y

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Fri Mar 27 00:00:00 UTC 2020

Abrogation of HLA surface expression using CRISPR/Cas9 genome editing: a step toward universal T cell therapy.

Scientific reports

Lee J,Sheen JH,Lim O,Lee Y,Ryu J,Shin D,Kim YY,Kim M

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Tue Oct 20 00:00:00 UTC 2020

Ghrelin ameliorates tumor-induced adipose tissue atrophy and inflammation via Ghrelin receptor-dependent and -independent pathways.

Oncotarget

Liu H,Luo J,Guillory B,Chen JA,Zang P,Yoeli JK,Hernandez Y,Lee II,Anderson B,Storie M,Tewnion A,Garcia JM

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Immunohistochemistry

Tue Sep 01 00:00:00 UTC 2020

Mucosal-associated invariant T (MAIT) cells mediate protective host responses in sepsis.

eLife

Trivedi S,Labuz D,Anderson CP,Araujo CV,Blair A,Middleton EA,Jensen O,Tran A,Mulvey MA,Campbell RA,Hale JS,Rondina MT,Leung DT

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Mon Nov 09 00:00:00 UTC 2020

Mucosal-associated invariant T (MAIT) cells mediate protective host responses in sepsis.

eLife

Trivedi S,Labuz D,Anderson CP,Araujo CV,Blair A,Middleton EA,Jensen O,Tran A,Mulvey MA,Campbell RA,Hale JS,Rondina MT,Leung DT

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Mon Nov 09 00:00:00 UTC 2020

CAIX Regulates GBM Motility and TAM Adhesion and Polarization through EGFR/STAT3 under Hypoxic Conditions.

International journal of molecular sciences

Huang BR,Liu YS,Lai SW,Lin HJ,Shen CK,Yang LY,Lu DY

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Fri Aug 14 00:00:00 UTC 2020

TLR2 on blood monocytes senses dengue virus infection and its expression correlates with disease pathogenesis.

Nature communications

Aguilar-Briseño JA,Upasani V,Ellen BMT,Moser J,Pauzuolis M,Ruiz-Silva M,Heng S,Laurent D,Choeung R,Dussart P,Cantaert T,Smit JM,Rodenhuis-Zybert IA

48-7349 was used in Flow cytometry/Cell sorting to demonstrate that TLR2, together with its co-receptors CD14 and TLR6, is an innate sensor of dengue virus particles.

Tue Jun 23 00:00:00 UTC 2020

Robust T Cell Response Toward Spike, Membrane, and Nucleocapsid SARS-CoV-2 Proteins Is Not Associated with Recovery in Critical COVID-19 Patients.

Cell reports. Medicine

Thieme CJ,Anft M,Paniskaki K,Blazquez-Navarro A,Doevelaar A,Seibert FS,Hoelzer B,Konik MJ,Berger MM,Brenner T,Tempfer C,Watzl C,Meister TL,Pfaender S,Steinmann E,Dolff S,Dittmer U,Westhoff TH,Witzke O,Stervbo U,Roch T,Babel N

48-7349 was used in Flow cytometry/Cell sorting to indicate that activation of differentiated memory effector T cells could cause hyperreactivity and immunopathogenesis in critical patients.

Tue Sep 22 00:00:00 UTC 2020

Persistence of dysfunctional natural killer cells in adults with high-functioning autism spectrum disorders: stigma/consequence of unresolved early infectious events?

Molecular autism

Bennabi M,Tarantino N,Gaman A,Scheid I,Krishnamoorthy R,Debré P,Bouleau A,Caralp M,Gueguen S,Le-Moal ML,Bouvard M,Amestoy A,Delorme R,Leboyer M,Tamouza R,Vieillard V

48-7349 was used in Immunocytochemistry-immunoflourescence to explore the phenotype and polyfunctional capacities of NK cells according to infectious stigma and clinical parameters.

Wed Jun 17 00:00:00 UTC 2020

PD1 is expressed on exhausted T cells as well as virus specific memory CD8+ T cells in the bone marrow of myeloma patients.

Oncotarget

Sponaas AM,Yang R,Rustad EH,Standal T,Thoresen AS,Dao Vo C,Waage A,Slørdahl TS,Børset M,Sundan A

48-7349 was used in Flow cytometry to study the expression of PD-1 on exhausted T cells as well as virus specific memory CD8+ T cells in the bone marrow of myeloma patients.

Fri Aug 10 00:00:00 UTC 2018

A single centre phase II trial to assess the immunological activity of TroVax® plus pemetrexed/cisplatin in patients with malignant pleural mesothelioma - the SKOPOS trial.

Oncoimmunology

Lester JF,Casbard AC,Al-Taei S,Harrop R,Katona L,Attanoos RL,Tabi Z,Griffiths GO

48-7349-42 was used in Flow Cytometry to reveal a circulating baseline immune signature that was significantly associated with long-term (>20 months in n = 8/23, 34.8%) survival.

Sat Feb 10 00:00:00 UTC 2024

Oncostatin M drives intestinal inflammation and predicts response to tumor necrosis factor-neutralizing therapy in patients with inflammatory bowel disease.

Nature medicine

West NR,Hegazy AN,Owens BMJ,Bullers SJ,Linggi B,Buonocore S,Coccia M,Görtz D,This S,Stockenhuber K,Pott J,Friedrich M,Ryzhakov G,Baribaud F,Brodmerkel C,Cieluch C,Rahman N,Müller-Newen G,Owens RJ,Kühl AA,Maloy KJ,Plevy SE,Keshav S,Travis SPL,Powrie F

48-7349 was used in Flow cytometry/Cell sorting to study how cytokine oncostatin M is a potential biomarker and therapeutic target for IBD, and has particular relevance for anti-TNF-resistant patients.

Mon May 01 00:00:00 UTC 2017

Heterogeneous Tumor-Immune Microenvironments among Differentially Growing Metastases in an Ovarian Cancer Patient.

Cell

Jiménez-Sánchez A,Memon D,Pourpe S,Veeraraghavan H,Li Y,Vargas HA,Gill MB,Park KJ,Zivanovic O,Konner J,Ricca J,Zamarin D,Walther T,Aghajanian C,Wolchok JD,Sala E,Merghoub T,Snyder A,Miller ML

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Thu Aug 24 00:00:00 UTC 2017

The distribution and function of human memory T cell subsets in lung cancer.

Immunologic research

Sheng SY,Gu Y,Lu CG,Zou JY,Hong H,Wang R

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Thu Jun 01 00:00:00 UTC 2017

Application of a whole blood mycobacterial growth inhibition assay to study immunity against Mycobacterium tuberculosis in a high tuberculosis burden population.

PloS one

Baguma R,Penn-Nicholson A,Smit E,Erasmus M,Day J,Makhethe L,de Kock M,Hughes EJ,van Rooyen M,Pienaar B,Stone L,Hanekom W,Brennan MJ,Wallis RS,Hatherill M,Scriba TJ

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Wed Oct 18 00:00:00 UTC 2017

A Quantitative Analysis of Complexity of Human Pathogen-Specific CD4 T Cell Responses in Healthy M. tuberculosis Infected South Africans.

PLoS pathogens

Lindestam Arlehamn CS,McKinney DM,Carpenter C,Paul S,Rozot V,Makgotlho E,Gregg Y,van Rooyen M,Ernst JD,Hatherill M,Hanekom WA,Peters B,Scriba TJ,Sette A

48-7349 was used in Flow cytometry/Cell sorting to estimate the breadth of T cell responses for the first time in the context of an infection and human population setting.

Fri Jul 01 00:00:00 UTC 2016

Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells.

Journal of immunology (Baltimore, Md. : 1950)

Marquardt N,Béziat V,Nyström S,Hengst J,Ivarsson MA,Kekäläinen E,Johansson H,Mjösberg J,Westgren M,Lankisch TO,Wedemeyer H,Ellis EC,Ljunggren HG,Michaëlsson J,Björkström NK

48-7349 was used in Flow cytometry/Cell sorting to study the unique lineage of CD49a(+)DX5(-) NK cells, showing that they are readily detectable in the liver but not in hepatic venous or peripheral blood.

Sun Mar 15 00:00:00 UTC 2015

NCR(+)ILC3 concentrate in human lung cancer and associate with intratumoral lymphoid structures.

Nature communications

Carrega P,Loiacono F,Di Carlo E,Scaramuccia A,Mora M,Conte R,Benelli R,Spaggiari GM,Cantoni C,Campana S,Bonaccorsi I,Morandi B,Truini M,Mingari MC,Moretta L,Ferlazzo G

48-7349 was used in Flow cytometry/Cell sorting to investigate the role of the NCR(+) ILC-3 in non-small cell lung cancer.

Wed Sep 23 00:00:00 UTC 2015

Targeting aPKC disables oncogenic signaling by both the EGFR and the proinflammatory cytokine TNFα in glioblastoma.

Science signaling

Kusne Y,Carrera-Silva EA,Perry AS,Rushing EJ,Mandell EK,Dietrich JD,Errasti AE,Gibbs D,Berens ME,Loftus JC,Hulme C,Yang W,Lu Z,Aldape K,Sanai N,Rothlin CV,Ghosh S

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Tue Aug 12 00:00:00 UTC 2014

Targeting aPKC disables oncogenic signaling by both the EGFR and the proinflammatory cytokine TNFα in glioblastoma.

Science signaling

Kusne Y,Carrera-Silva EA,Perry AS,Rushing EJ,Mandell EK,Dietrich JD,Errasti AE,Gibbs D,Berens ME,Loftus JC,Hulme C,Yang W,Lu Z,Aldape K,Sanai N,Rothlin CV,Ghosh S

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Tue Aug 12 00:00:00 UTC 2014

Targeting aPKC disables oncogenic signaling by both the EGFR and the proinflammatory cytokine TNFα in glioblastoma.

Science signaling

Kusne Y,Carrera-Silva EA,Perry AS,Rushing EJ,Mandell EK,Dietrich JD,Errasti AE,Gibbs D,Berens ME,Loftus JC,Hulme C,Yang W,Lu Z,Aldape K,Sanai N,Rothlin CV,Ghosh S

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Tue Aug 12 00:00:00 UTC 2014

Targeting aPKC disables oncogenic signaling by both the EGFR and the proinflammatory cytokine TNFα in glioblastoma.

Science signaling

Kusne Y,Carrera-Silva EA,Perry AS,Rushing EJ,Mandell EK,Dietrich JD,Errasti AE,Gibbs D,Berens ME,Loftus JC,Hulme C,Yang W,Lu Z,Aldape K,Sanai N,Rothlin CV,Ghosh S

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Tue Aug 12 00:00:00 UTC 2014

Dendritic cells restore CD8+ T cell reactivity to autologous HIV-1.

Journal of virology

Smith KN,Mailliard RB,Larsen BB,Wong K,Gupta P,Mullins JI,Rinaldo CR

48-7349 was used in Flow cytometry/Cell sorting to demonstrate that antigen-presenting cells can be used to identify antigen-specific T cell reactivity in HIV-1 infection.

Mon Sep 01 00:00:00 UTC 2014

Functional avidity and IL-2/perforin production is linked to the emergence of mutations within HLA-B*5701-restricted epitopes and HIV-1 disease progression.

Journal of immunology (Baltimore, Md. : 1950)

Buggert M,Norström MM,Salemi M,Hecht FM,Karlsson AC

48-7349 was used in Flow cytometry/Cell sorting to distinguish whether specific functional characteristics of HLA-B*5701-restricted CD8+ T-cells influence the emergence of mutations in high-risk progressors versus low-risk progressors.

Thu May 15 00:00:00 UTC 2014

Transcriptional profile of tuberculosis antigen-specific T cells reveals novel multifunctional features.

Journal of immunology (Baltimore, Md. : 1950)

Arlehamn CL,Seumois G,Gerasimova A,Huang C,Fu Z,Yue X,Sette A,Vijayanand P,Peters B

48-7349 was used in Flow cytometry/Cell sorting to study the role of CD4(+) T cells in latent tuberculosis infection, showing that tuberculosis antigen-specific T cells have novel multifunctional features.

Mon Sep 15 00:00:00 UTC 2014

Cell-mediated immune responses to respiratory syncytial virus infection: magnitude, kinetics, and correlates with morbidity and age.

Human vaccines & immunotherapeutics

Geevarghese B,Weinberg A

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Mon Apr 25 00:00:00 UTC 2016

Mucosal immunization of lactating female rhesus monkeys with a transmitted/founder HIV-1 envelope induces strong Env-specific IgA antibody responses in breast milk.

Journal of virology

Fouda GG,Amos JD,Wilks AB,Pollara J,Ray CA,Chand A,Kunz EL,Liebl BE,Whitaker K,Carville A,Smith S,Colvin L,Pickup DJ,Staats HF,Overman G,Eutsey-Lloyd K,Parks R,Chen H,Labranche C,Barnett S,Tomaras GD,Ferrari G,Montefiori DC,Liao HX,Letvin NL,Haynes BF,Permar SR

48-7349-42 was used in Flow Cytometry to show that systemic MVA prime followed by either intranasal or systemic protein boosts can elicit strong humoral responses in breast milk and may be a useful strategy to interrupt postnatal HIV-1 transmission.

Sat Jun 01 00:00:00 UTC 2013

CD4⁺CD73⁺ T cells are associated with lower T-cell activation and C reactive protein levels and are depleted in HIV-1 infection regardless of viral suppression.

AIDS (London, England)

Schuler PJ,Macatangay BJ,Saze Z,Jackson EK,Riddler SA,Buchanan WG,Hilldorfer BB,Mellors JW,Whiteside TL,Rinaldo CR

48-7349 was used in Flow cytometry/Cell sorting to assess suppression of T-cell function by exogenous ADO and CD4+ CD73+ T cells.

Wed Jun 19 00:00:00 UTC 2013

Functional OCT4-specific CD4⁺ and CD8⁺ T cells in healthy controls and ovarian cancer patients.

Oncoimmunology

Di J,Massuger LF,Duiveman-de Boer T,Zusterzeel PL,Figdor CG,Torensma R

Published figure using TNF alpha monoclonal antibody (Product # 48-7349-42) in Flow Cytometry

Wed May 01 00:00:00 UTC 2013

Tim-3 expression on PD-1+ HCV-specific human CTLs is associated with viral persistence, and its blockade restores hepatocyte-directed in vitro cytotoxicity.

The Journal of clinical investigation

McMahan RH,Golden-Mason L,Nishimura MI,McMahon BJ,Kemper M,Allen TM,Gretch DR,Rosen HR

48-7349 was used in Flow cytometry/Cell sorting to indicate the coexpression of Tim-3 and PD-1 correlates with defective T-cell responses and may limit immune control of persistent pathogens.

Wed Dec 01 00:00:00 UTC 2010

生物信息学

蛋白别名: APC1 protein; Cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; tumor necrosis factor ligand 1F; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha

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基因别名: DIF; TNF; TNF-alpha; TNFA; TNFSF2; TNLG1F

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UniProt ID: (Human) P01375

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Entrez Gene ID: (Human) 7124

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功能

protease binding cytokine activity tumor necrosis factor receptor binding protein binding identical protein binding transcription regulatory region DNA binding

参与通路