Invitrogen
应用 | 建议稀释比 | 已发表文章 |
---|---|---|
免疫印迹 (WB) |
- | 查看1篇已发表文章 1篇已发表文章 |
流式细胞分析 (Flow) |
0.25 µg/test | - |
酶联免疫吸附实验 (ELISA) |
- | 查看1篇已发表文章 1篇已发表文章 |
中和实验 (Neu) |
- | 查看1篇已发表文章 1篇已发表文章 |
产品规格 | |
---|---|
种属反应 |
Mouse, Rat |
已发表种属 |
Not Applicable |
宿主/亚型 |
Armenian hamster / IgG |
推荐的同型对照 |
Armenian Hamster IgG Isotype Control (eBio299Arm), FITC, eBioscience™ |
分类 |
Monoclonal |
类型 |
Antibody |
克隆号 |
TN3-19.12 |
偶联物 |
FITC
|
激发/发射光谱 |
查看光谱 |
形式 |
Liquid |
浓度 |
0.5 mg/mL |
纯化类型 |
Affinity chromatography |
保存液 |
PBS, pH 7.2 |
内含物 |
0.09% sodium azide |
保存条件 |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_465427 |
Description: The TN3-19.12 monoclonal antibody reacts with mouse, rat, and rabbit tumor necrosis factor-alpha (TNF alpha), but not with human TNF alpha. The TN3-19.12 antibody is a neutralizing antibody. Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF alpha is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF alpha have been observed.
Applications Reported: The TN3-19.12 antibody has been reported for use in intracellular staining for flow cytometric analysis, cytokine neutralization, and ELISA. Fluorochrome conjugated TN3-19.12 is recommended for use in intracellular staining and flow cytometry. Functional Grade purified TN3-19.12, cat. 16-7423, is recommended for use in functional assays. For ELISPOT capture, the alternative clone, 1F3F3D4 (cat # 16-7325), is recommended.
Applications Tested: This TN3-19.12 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes. It is offered in 2 formats: - µg size: can be used at less than or equal to 0.25 µg per test.- test size: can be used at 5 µL (0.125 µg) per test.
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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蛋白别名: C-domain 1; C-domain 2; Cachectin; DADB-70P7.1; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; TNF alpha; TNF superfamily; TNF α; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha
基因别名: DIF; RATTNF; Tnf; TNF-a; TNF-alpha; Tnfa; TNFalpha; Tnfsf1a; TNFSF2
UniProt ID: (Rat) P16599, (Mouse) P06804
Entrez Gene ID: (Rat) 24835, (Mouse) 21926