Invitrogen

TPI1 Monoclonal Antibody (P3711)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他16个TPI1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

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TPI1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of TPI1 was performed in paraffin-embedded mouse testis tissue using TPI1 Monoclonal Antibody (P3711) (Product # MA5-18297) at a dilution of 1:100.

TPI1 Antibody in Western Blot (WB) — TPI1 Monoclonal Antibody (P3711) detects Triosephosphate isomerase protein by western blot analysis. A. 30 µg Neuro2A whole cell extract. B. 30 µg C8D30 whole cell extract. C. 30 µg NIH-3T3 whole cell extract. D. 30 µg Raw264.7 whole cell extract. E. 30 µg C2C12 whole cell extract.12 % SDS-PAGE. TPI1 Monoclonal Antibody (P3711) (Product # MA5-18297) dilution: 1:1,000.

Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. 293T cells were transfected with TPI1 siRNA and decrease in signal intensity was observed in western blot application using TPI1 Antibody (Product # (MA5-18297). {KD}

产品信息

MA5-18297

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:3,000	-
免疫组化（石蜡） (IHC (P))	1:100-1:1,000	-

产品规格
种属反应	Human, Mouse, Rat
宿主/亚型	Mouse / IgG2a
分类	Monoclonal
类型	Antibody
克隆号	P3711
抗原	Recombinant fragment corresponding to a region within amino acids 187 and 249 of Triosephosphate isomerase 查看免疫原
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Protein G
保存液	PBS
内含物	no preservative
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2539671

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2.

Predicted reactivity: Mouse (100%), Rat (100%), Dog (100%), Chimpanzee (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate and dihydroxy-acetone phosphate in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。