Invitrogen

TRAF3 Recombinant Rabbit Monoclonal Antibody (12H13L59)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1个已发表图片

4 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

TRAF3 Antibody in Immunocytochemistry (ICC/IF)

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TRAF3 Antibody (700121) in ICC/IF

Immunofluorescent analysis of TRAF3 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with TRAF3 Recombinant Rabbit Monoclonal Antibody (Product # 700121) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Pa... View More

TRAF3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TRAF3 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A549 cells were transfected with TRAF3 siRNA and loss of signal was observed in Western Blot using Anti-TRAF3 Monoclonal Antibody (Product # 700121). {KD}

产品信息

700121

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

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免疫组化（石蜡） (IHC (P))

1:10-1:100

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

12H13L59

抗原

A recombinant protein corresponding to amino acids 1-187 of Q13114.

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偶联物

Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2532283

产品详细信息

This antibody is predicted to react with chimpanzee, Rhesus monkey, mouse, rat, porcine, canine, bovine and equine based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e. g. RANK, CD30, CD40, etc. ) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6). TRAF3, originally named CRAF1, interacts directly with the CD40 cytoplasmic tail through a region of similarity to the tumor necrosis factor-alpha (TNF-alpha) receptor-associated factors. TRAF3 binds only a single site, which contains the sequence PEQET, whereas TRAF1 and TRAF2 are capable of binding to either the PEQET site or an additional downstream domain.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A20 undermines alternative NF-κB activity and expression of anti-apoptotic genes in Helicobacter pylori infection.

Cellular and molecular life sciences : CMLS

Lim MCC,Maubach G,Birkl-Toeglhofer AM,Haybaeck J,Vieth M,Naumann M

700121 was used in Western Blot to report that the classical NF-κB target gene product A20 contributes to the negative regulation of alternative NF-κB signaling in gastric epithelial cells infected by Helicobacter pylori.

Fri Jan 28 00:00:00 EST 2022

TIFA has dual functions in Helicobacter pylori-induced classical and alternative NF-κB pathways.

EMBO reports

Maubach G,Lim MCC,Sokolova O,Backert S,Meyer TF,Naumann M

Published figure using TRAF3 recombinant monoclonal antibody (Product # 700121) in Western Blot

Mon Sep 06 00:00:00 EDT 2021

Cellular inhibitors of apoptosis are global regulators of NF-κB and MAPK activation by members of the TNF family of receptors.

Science signaling

Varfolomeev E,Goncharov T,Maecker H,Zobel K,Kömüves LG,Deshayes K,Vucic D

Tue Mar 20 00:00:00 EDT 2012

Cellular inhibitors of apoptosis are global regulators of NF-κB and MAPK activation by members of the TNF family of receptors.

Science signaling

Varfolomeev E,Goncharov T,Maecker H,Zobel K,Kömüves LG,Deshayes K,Vucic D

Tue Mar 20 00:00:00 EDT 2012

生物信息学

蛋白别名: CD40 associated protein 1; CD40 binding protein; CD40 receptor associated factor 1; CD40 receptor-associated factor 1; CD40-binding protein; CD40BP; CRAF1; LAP1; LMP1-associated protein 1; RING-type E3 ubiquitin transferase TRAF3; TNF receptor-associated factor 3

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基因别名: CAP-1; CAP1; CD40bp; CRAF1; IIAE5; LAP1; TRAF3; TRAFAMN

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UniProt ID: (Human) Q13114

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Entrez Gene ID: (Human) 7187

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功能