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Invitrogen
TRAP1 Monoclonal Antibody (Trap1-6), APC
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TRAP1 Antibody (MA5-45269) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using PC3M cells. Samples incubated with TRAP1 monoclonal antibody (Product # MA5-45269). Counterstain used was Mitotracker (red) mitochondria stain. DAPI (blue) nuclear stain. (A) staining of mitochondria with MitoTracker dye; (B) staining of nuclei with DAPI; (C) staining with TRAP1 antibody; (D) tricolor composite of images A-C.
产品信息
MA5-45269
产品规格
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
Trap1-6
抗原
Purified recombinant full length TRAP1 (no tags)
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
1 µg/mL of MA5-45269 was sufficient for detection of Trap-1/HSP75 in 20 µg of Hela Cell lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 75kDa.
靶标信息
The 90 kDa heat shock protein (hsp90) family of molecular chaperones is a highly conserved family of proteins that play an important physiological role. Hsp90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of hsp90 is the tumor necrosis factor receptor-associated protein (TRAP1). Like hsp90, TRAP1 is found to be associated with numerous proteins involved in diverse actions. Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting presequence strongly implicates TRAP1 as a mitochondrial matrix protein.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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