Invitrogen

TSG101 Polyclonal Antibody

Name: TSG101 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-31260

高级验证

17个已发表图片

22 篇参考文献

查看其他24个TSG101抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

TSG101 Antibody in Immunocytochemistry (ICC/IF)

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TSG101 Antibody (PA5-31260) in ICC/IF

Immunofluorescence analysis of Tsg101 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for overnight at room temperature. The cells were labeled with TSG101 Polyclonal Antibody (Product # PA5-31260, 1:100( in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32790, 1:200... View More

TSG101 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TSG101 Antibody in Immunoprecipitation (IP)

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产品信息

PA5-31260

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看20篇已发表文章 20篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100

酶联免疫吸附实验 (ELISA)

1:1,000-1:10,000

免疫沉淀 (IP)

1:100-1:500

亲和纯化 (AP)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Bovine, C. elegans, Horse, Human, Mouse, Zebrafish

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human TSG101. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.42 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2548734

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, K562, THP-1, HL-60, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, A549, H1299, HCT116, U87-MG, SK-N-SH, IMR32, SK-N-AS.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

TSG101-4A10 recognizes TSG101 protein, the product of a recently identified Tumor Susceptibility Gene whose inactivation in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice. The TSG101 gene encodes a 46 kDa protein and contains a proline-rich domain and a leucine-zipper motif within a coiled-coil domain near the carboxyl terminus.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: ESCRT-I complex subunit TSG101; ESCRTI complex subunit TSG101; TSG 101; Tumor supressor; tumor susceptibility gene 10; tumor susceptibility gene 101; Tumor susceptibility gene 101 protein; tumor susceptibility protein

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基因别名: AI255943; CC2; Rw; TSG10; TSG101; VPS23

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UniProt ID: (Human) Q99816, (Rat) Q6IRE4, (Mouse) Q61187

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Entrez Gene ID: (Human) 7251, (Rat) 292925, (Mouse) 22088

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功能