Invitrogen

TSG101 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1个已发表图片

3 篇参考文献

查看其他25个TSG101抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

TSG101 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

TSG101 Antibody (PA5-82236) in ICC/IF

Immunofluorescence analysis of Tsg101 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TSG101 Polyclonal Antibody (Product # PA5-82236) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32790), (1:20... View More

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产品信息

PA5-82236

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

1:200-1:500

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human TSG101. Recombinant protein control fragment (Product #RP-89296).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Anitgen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2789397

产品详细信息

Immunogen sequence: RMKEEMDRAQ AELNALKRTE EDLKKGHQKL EEMVTRLDQE VAEVDKNIEL LKKKDEELSS ALEKMENQSE NNDIDEVIIP TAPLYKQILN LYAEENAIED TIFYLGEALR RGVIDLDVFL KHVRLLS

靶标信息

TSG101-4A10 recognizes TSG101 protein, the product of a recently identified Tumor Susceptibility Gene whose inactivation in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice. The TSG101 gene encodes a 46 kDa protein and contains a proline-rich domain and a leucine-zipper motif within a coiled-coil domain near the carboxyl terminus.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Small Extracellular Vesicles from Peripheral Blood of Aged Mice Pass the Blood-Brain Barrier and Induce Glial Cell Activation.

Cells

Morales-Prieto DM,Murrieta-Coxca JM,Stojiljkovic M,Diezel C,Streicher PE,Henao-Restrepo JA,Röstel F,Lindner J,Witte OW,Weis S,Schmeer C,Marz M

Published figure using TSG101 polyclonal antibody (Product # PA5-82236) in Western Blot

Fri Feb 11 00:00:00 EST 2022

Small Extracellular Vesicles from Peripheral Blood of Aged Mice Pass the Blood-Brain Barrier and Induce Glial Cell Activation.

Cells

Morales-Prieto DM,Murrieta-Coxca JM,Stojiljkovic M,Diezel C,Streicher PE,Henao-Restrepo JA,Röstel F,Lindner J,Witte OW,Weis S,Schmeer C,Marz M

Published figure using TSG101 polyclonal antibody (Product # PA5-82236) in Western Blot

Fri Feb 11 00:00:00 EST 2022

MicroRNA-370 carried by M2 macrophage-derived exosomes alleviates asthma progression through inhibiting the FGF1/MAPK/STAT1 axis.

International journal of biological sciences

Li C,Deng C,Zhou T,Hu J,Dai B,Yi F,Tian N,Jiang L,Dong X,Zhu Q,Zhang S,Cui H,Cao L,Shang Y

PA5-82236 was used in Western Blot to conclude that M2Φ-Exos carry miR-370 to alleviate asthma progression through downregulating FGF1 expression and the MAPK/STAT1 signaling pathway.

Wed Mar 30 00:00:00 EDT 2022

Cancer-derived exosomal TRIM59 regulates macrophage NLRP3 inflammasome activation to promote lung cancer progression.

Journal of experimental & clinical cancer research : CR

Liang M,Chen X,Wang L,Qin L,Wang H,Sun Z,Zhao W,Geng B

PA5-82236 was used in Western Blotting to indicate that tumor-derived exosomal TRIM59 converts macrophages to tumor-promoting functions of macrophages via regulating ABHD5 proteasomal degradation, to activate NLRP3 inflammasome signaling pathway to promote lung cancer progression by IL-1β secretion.

Mon Aug 31 00:00:00 EDT 2020

生物信息学

蛋白别名: ESCRT-I complex subunit TSG101; ESCRTI complex subunit TSG101; TSG 101; Tumor supressor; tumor susceptibility gene 10; tumor susceptibility gene 101; Tumor susceptibility gene 101 protein; tumor susceptibility protein

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基因别名: AI255943; CC2; Rw; TSG10; TSG101; VPS23

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UniProt ID: (Human) Q99816, (Rat) Q6IRE4, (Mouse) Q61187

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Entrez Gene ID: (Human) 7251, (Rat) 292925, (Mouse) 22088

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功能

DNA binding transcription corepressor activity protein binding ligand-dependent nuclear receptor transcription coactivator activity ubiquitin protein ligase binding protein homodimerization activity ubiquitin binding virion binding calcium-dependent protein binding ubiquitin-protein ligase

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