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Invitrogen
TSLP Polyclonal Antibody, Biotin, PeproTech®
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TSLP Antibody (500-P258BT-25UG) in ELISA
Sandwich ELISA: To detect hTSLP by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of TSLP Polyclonal Antibody, Biotin (Product # 500-P258BT-500UG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech TSLP Polyclonal Antibody (Product # 500-P258-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hTSLP.
产品信息
500-P258BT-25UG
产品规格
种属反应
Human
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived, 15.0 kDa Recombinant Human TSLP
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
产品详细信息
AA Sequence of recombinant protein: MYDFTNCDFE KIKAAYLSTI SKDLITYMSG TKSTEFNNTV SCSNRPHCLT EIQSLTFNPTAGCASLAKEM FAMKTKAALA IWCPGYSETQ INATQAMKKR RKRKVTTNKC LEQVSQLQGLWRRFNRPLLK QQ.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TSLP. Anti-Human TSLP-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hTSLP by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TSLP (500-P258) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hTSLP.
Western Blot: To detect hTSLP by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTSLP is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P258BT-1MG will be provided as 2 x 500 µg
靶标信息
TSLP is a 159 amino acid four helix-bundle hemopoietic cytokine that signals through a heterodimeric receptor complex consisting of the human TSLP receptor and the IL-7R alpha-chain. It impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes, enhances the maturation of CD11c (+) dendritic cells, naive CD4 (+), and CD8 (+) T cells into proallergic effectors, and primes dendritic cells to produce large amounts of IL-12 after CD40 ligand stimulation. TSLP influences the regulation of the positive selection of regulatory T cells and maintenance of peripheral CD4 (+) T cell homeostasis. It induces allergic inflammation by directly activating mast cells and may be involved in the pathophysiology of inflammatory arthritis. It is expressed in heart, liver and prostate.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Thymic stromal lymphopoietin
基因别名: TSLP
UniProt ID: (Human) Q969D9
Entrez Gene ID: (Human) 85480
