Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
TagRFP Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
图: 1 / 4
TagRFP Antibody (R10367) in ICC/IF
Immunofluorescent analysis of RFP Tag was performed using 70% confluent p65-mRFP transfected HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with RFP Polyclonal Antibody (Product # R10367) at1:2500 dilution and NFkB p65 Monoclonal Antibody (2A12A7) (Product # 33-9900) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG ... View More
产品信息
R10367
应用
建议稀释比
已发表文章
产品规格
已发表种属
C. elegans,
Tag
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Full-length recombinant denatured and non-denatured TagRFP was used as the immunogen
偶联物
形式
Lyophilized
保存条件
-20°C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody can be used to detect native RFP, RFP variants, and RFP fusion proteins in Western blot, immunoprecipitation, ELISA, and immunocytochemistry. This antibody has been shown to detect denatured and native TurboRFP, TurboFP602, TurboFP635, TagRFP, TagFP635, and mKate2.
Storage and reconstitution: to prepare a 1 mg/mL stock solution, add 100 µL sterile water or 50% glycerol to the vial. If desired, aliquot the antibody upon reconstitution to produce single-use samples. Store the reconstituted product with sterile water at 4° C, avoiding freeze/thaw cycles. When stored as directed, the solution is stable for 3 months. If reconstituted with 50% glycerol, store at -20° C, avoiding freeze/thaw cycles. When stored as directed, this solution is stable for 6 months.
靶标信息
Red fluorescent protein (TagRFP) derived from Entacmaea quadricolor eqFP611 (Uniprot ID: Q8ISF8) is an important tool in biotechnology and cell biology as a spectrally distinct companion or substitute for the green fluorescent protein (GFP; from Aequorea jellyfish). Pigment protein. The emission does not change over the pH range of 4 to 10. Maturation occurs via a green intermediate and is complete within 12 hours. Recombinant expression at high temperatures induces oligomerization. Abs(max)=559 nm. Exhibits a smaller absorbance peak at 525 nm. Has a strong fluorescence emission spectrum which peaks at 611 nm. [Uniprot]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
