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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • UCP3 Antibodies

          Invitrogen

          UCP3 Polyclonal Antibody

          高级验证
          10个已发表图片
          27 篇参考文献
          查看其他6个UCP3抗体

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          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite UCP3 Polyclonal Antibody

          • 抗体检测数据 (1)
          • 已发表图片 (10)
          • 高级验证 (1)
          UCP3 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          UCP3 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          图: 1 / 12

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          UCP3 Antibody (PA1-055) in WB

          Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of L6 (Lane 1) and C2C12 (Lane 2). The blot was probed with Anti-UCP3 Rabbit Polyclonal Antibody (Product # PA1-055, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 34 kDa band corresponding to UCP3 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® ; 12% Bis-... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          UCP3 Antibody in Western Blot (WB)
          UCP3 Antibody in Western Blot (WB)
          UCP3 Antibody in Western Blot (WB)
          UCP3 Antibody in Western Blot (WB)
          UCP3 Antibody in Western Blot (WB)
          UCP3 Antibody in Western Blot (WB)
          UCP3 Antibody in Western Blot (WB)
          UCP3 Antibody in Western Blot (WB)
          UCP3 Antibody in Western Blot (WB)
          UCP3 Antibody in Western Blot (WB)
          UCP3 Antibody in Western Blot (WB)
          UCP3 Antibody

          产品信息

          PA1-055

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          0.5-2 µg/mL
          查看26篇已发表文章 26篇已发表文章

          其他PubMed (Misc)

          -
          查看1篇已发表文章 1篇已发表文章
          产品规格

          种属反应

          Dog, Mouse, Rat

          已发表种属

          Human, Mouse, Rat

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          Synthetic peptide corresponding to residues C R(295) A L M K V Q V L R E S P F(308) of mouse and rat UCP-3.
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          1 mg/mL

          纯化类型

          Antigen affinity chromatography

          保存液

          PBS with 1mg/mL BSA

          内含物

          0.05% sodium azide

          保存条件

          -20°C, Avoid Freeze/Thaw Cycles

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2213925

          产品详细信息

          PA1-055 detects uncoupling protein-3 (UCP-3) mouse, rat, and canine tissues. This antibody will also detect recombinant human UCP-3, but does not detect UCP-3 from mouse liver or kidney.

          PA1-055 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~30 kDa protein representing UCP-3 from rat heart homogenate.

          The PA1-055 immunogen is a synthetic peptide corresponding to residues C R(295) A L M K V Q V L R E S P F(308) of mouse and rat UPC-3. This peptide differs from the porcine and human UCP-3 sequence by a single amino acid substitution. PA1-055 immunizing peptide (Cat. # PEP-074) is available for use in neutralization and control experiments.

          靶标信息

          The uncoupling protein UCP1 (formerly designated UCP) is an integral membrane protein unique to brown adipose tissue mitochondria. UCP1 forms a dimer that acts as a proton channel, which can uncouple oxidative phosphorylation by dissipating the electrochemical potential across the inner mitochondrial membrane. This process induces heat production in brown adipose tissue and is involved in regulation of body temperature and glucose metabolism. UCP2 is a structurally related protein that also uncouples mitochondrial respiration. It is more widely expressed in human and mouse tissues, including white adipose tissue and muscle, than is UCP1. UCP2 is thought to play a role in body weight regulation. An additional UCP family member, UCP3, is highly muscle specific and is possibly involved in the uncoupling of oxidative phosphorylation in skeletal muscle.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: mitochondrial uncoupling protein 3; Putative mitochondrial transporter UCP3; Solute carrier family 25 member 9; UCP 3; uncoupling protein; Uncoupling protein 3; Uncoupling protein 3, mitochondrial; Uncoupling protein-3

          查看更多 收起

          基因别名: AI645527; SLC25A9; UCP-3; UCP3

          查看更多 收起

          UniProt ID: (Dog) Q9N2I9, (Mouse) P56501, (Rat) P56499

          查看更多 收起

          Entrez Gene ID: (Dog) 403575, (Mouse) 22229, (Rat) 25708

          查看更多 收起

          功能
          oxidative phosphorylation uncoupler activity hydrogen ion transmembrane transporter activity transporter secondary carrier transporter
          参与通路
          hydrogen ion transmembrane transport response to superoxide response to hypoxia fatty acid metabolic process transport mitochondrial transport proton transport transmembrane transport aging response to nutrient response to cold response to activity response to insulin cellular response to hormone stimulus response to steroid hormone response to glucocorticoid
          It has to be done as per old AB suggested Products section.
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          性能保障

          只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符,您即可享受免费退换货服务。*

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