Invitrogen

UHMK1 Polyclonal Antibody

Product Image Gallery

UHMK1 Antibody in Western Blot (WB) — LncRNA H19-induced UHMK1 is highly expressed in PDAC. (A) Volcano plot showing differentially expressed genes in MIA-PaCa2 cell line depleted for lncRNA H19 using the siRNAs siH19-1 (left panel) and siRNAs siH19-2 (right panel), in comparison with cells transfected with a nonsense siRNA control (NC). The threshold was set to a log 2 (fold change) >1 and a P value <0.05. Vertical axis corresponds to the statistical significance level provided as the -Log10 P value. The horizontal dashed gray line shows the P-value cutoff (-log10 1.3 P = 0.05) with points above the line having P values <0.05. The vertical gray dashed line indicates 1-fold changes/doubling (log2-fold change of 1), The six most significantly differentially regulated genes were MIGA1, SERPINB9, SGPL1, ZNF616, ZNF56, and UHMK1. (B) UHMK1 mRNA expression in the PDAC cell lines BxPc-3, AsPC-1 and MIA-PaCa2 after depletion of lncRNA H19, compared with control cells (NC). (C) UHMK1 mRNA expression in CRL-4023 (CRL), and the PDAC cell lines BxPc-3, AsPC-1, MIA-PaCa2, PANC-1, and BxGEM, The data were normalized to the expression of CRL-4023 cells. (D) GAPDH served as a loading control. The protein sizes in kilodaltons (kDa) are shown on the right. The crude Western blot images are shown in Figure S2. (E) UHMK1 protein expression was detected in paraffin-embedded human tissue derived from PDAC (n = 20) or nonmalignant (normal) pancreata (n = 20) by performing immunohistochemistry. Cell nuclei were stained with hematoxylin

产品信息

PA5-50622

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:200-1:1,000	查看1篇已发表文章 1篇已发表文章

产品规格
种属反应	Human, Mouse, Rat
已发表种属	Not Applicable
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthetic peptide corresponding to residues near the C terminal of human U2AF homology motif (UHM) kinase 1 查看免疫原
偶联物	Unconjugated
形式	Liquid
浓度	1.7 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.4, with 40% glycerol
内含物	0.05% sodium azide
保存条件	-20°C
RRID	AB_2636075

产品详细信息

The antibody detects endogenous levels of total UHMK1 protein.

靶标信息

Upon serum stimulation, KIS, a member of the Ser/Thr protein kinase family, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. This protein, which contains 1 RRM (RNA recognition motif)domain, may be involved in trafficking and/or processing of RNA. KIS is widely expressed, with highest levels in skeletal muscle, kidney, placenta and peripheral blood leukocytes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

UHMK1 Is a Novel Marker for Personalized Prediction of Pancreatic Cancer Prognosis.

Frontiers in oncology

Luo Y,Han S,Yan B,Ji H,Zhao L,Gladkich J,Herr I

Published figure using UHMK1 polyclonal antibody (Product # PA5-50622) in Western Blot

Sat Apr 02 00:00:00 EDT 2022

生物信息学

蛋白别名: kinase interacting stathmin; kinase interacting with leukemia-associated gene (stathmin); Kinase interacting with stathmin; KIS protein kinase; P-CIP2; PAM COOH-terminal interactor protein 2; Serine/threonine-protein kinase Kist; U2AF homology motif kinase 1

基因别名: 4732477C12Rik; 4930500M09Rik; AA673513; AI449218; AU021979; KIS; KIST; P-CIP2; UHMK1

UniProt ID: (Human) Q8TAS1, (Mouse) P97343, (Rat) Q63285

Entrez Gene ID: (Human) 127933, (Mouse) 16589, (Rat) 246332

分子生物学功能: non-receptor serine/threonine protein kinase protein modifying enzyme