Invitrogen

UHRF1 Polyclonal Antibody

View all (17) UHRF1 antibodies
Datasheet
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Datasheet
Protocols
Tech Support

Product Details

PA5-96109

Applications
Tested Dilution
Publications

免疫印迹 (WB)

1:500-1:2,000
-

免疫组化（石蜡） (IHC (P))

1:50-1:200
-

免疫细胞化学 (ICC/IF)

1:20-1:50
-
产品规格

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

分类

Polyclonal

类型

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human UHRF1.
View immunogen

Conjugate

Unconjugated

形式

Liquid

浓度

0.57 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2807911

Product Specific Information

Immunogen sequence: MWIQVRTMDG RQTHTVDSLS RLTKVEELRR KIQELFHVEP GLQRLFYRGK QMEDGHTLFD YEVRLNDTIQ LLVRQSLVLP HSTKERDSEL SDTDSGCCLG QSESDKSSTH GEAAAETDSR PADEDMWDET ELGLYKVNEY VDARDTNMGA WFEAQVVRVT RKAPSRDEPC SSTSRPALEE DVIYHVKYDD YPENGVVQMN SRDVRARART IIKWQDLEVG QVVMLNYNPD NPKERGFWYD AEISRKRETR TARELYANVV; Positive Samples: THP-1, HeLa, SW480, MCF7; Cellular Location: Nucleus

Target Information

This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Bioinformatics

Protein Aliases: Ac2-121; E3 ubiquitin-protein ligase UHRF1; FLJ21925; Hnp95 Huhrf1; hUHRF1; HuNp95; Inverted CCAAT box-binding protein of 90 kDa; MGC138707; mUhrf1; nuclear phosphoprotein 95; Nuclear protein 95; Nuclear zinc finger protein Np95; RING finger protein 106; RING-type E3 ubiquitin transferase UHRF1; Transcription factor ICBP90; ub; Ubiquitin-like PHD and RING finger domain-containing protein 1; ubiquitin-like with PHD and ring finger domains 1; Ubiquitin-like-containing PHD and RING finger domains protein 1

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Gene Aliases: AL022808; hNP95; hUHRF1; huNp95; ICBP90; NP95; RNF106; TDRD22; UHRF1

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UniProt ID: (Human) Q96T88, (Mouse) Q8VDF2

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Entrez Gene ID: (Human) 29128, (Mouse) 18140

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Molecular Function: ubiquitin-protein ligase

Function(s)
negative regulation of transcription from RNA polymerase II promoter DNA repair transcription, DNA-templated cell cycle cell proliferation maintenance of DNA methylation histone monoubiquitination histone ubiquitination positive regulation of cellular protein metabolic process protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of transcription from RNA polymerase II promoter protein autoubiquitination regulation of methylation-dependent chromatin silencing positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity regulation of transcription, DNA-templated cellular response to DNA damage stimulus chromatin modification
Process(es)
core promoter proximal region sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding ubiquitin-protein transferase activity protein binding zinc ion binding methyl-CpG binding ligase activity nucleosomal histone binding methylated histone binding histone binding identical protein binding hemi-methylated DNA-binding ubiquitin protein ligase activity DNA binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity
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