Invitrogen

UHRF1 Recombinant Rabbit Monoclonal Antibody (1H2L41)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他21个UHRF1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (2)
高级验证 (1)

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UHRF1 Antibody (702830) in WB

Western blot analysis was performed on Modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1) Hep G2 (Lane 2), HCT 116 (Lane 3), COLO 205 (Lane 4), Jurkat (Lane 5), MCF-7 (Lane 6), MDA-MB-231 (Lane 7), U-2 OS (Lane 8) and PANC-1 (Lane 9). The blots were probed with Anti-UHRF1 Recombinant Rabbit Monoclonal Antibody (Product # 702830, 1:200 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 90 kDa... View More

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UHRF1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF-7 cells were transfected with UHRF1 siRNA and decrease in signal intensity was observed in Western blot application using Anti-UHRF1 Recombinant Rabbit Monoclonal Antibody (Product # 702830). {KD}

产品信息

702830

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

1H2L41

抗原

Protein corresponding to human UHRF1 [126-285]

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偶联物

Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2725627

产品详细信息

This antibody is predicted to react with Monkey, Dog, Rat.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Ac2-121; E3 ubiquitin-protein ligase UHRF1; FLJ21925; Hnp95 Huhrf1; hUHRF1; HuNp95; Inverted CCAAT box-binding protein of 90 kDa; MGC138707; nuclear phosphoprotein 95; Nuclear protein 95; Nuclear zinc finger protein Np95; RING finger protein 106; RING-type E3 ubiquitin transferase UHRF1; Transcription factor ICBP90; ub; Ubiquitin-like PHD and RING finger domain-containing protein 1; ubiquitin-like with PHD and ring finger domains 1; Ubiquitin-like-containing PHD and RING finger domains protein 1

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基因别名: hNP95; hUHRF1; huNp95; ICBP90; NP95; RNF106; TDRD22; UHRF1

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UniProt ID: (Human) Q96T88

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Entrez Gene ID: (Human) 29128

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功能