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FIGURE: 1 / 1
USP16 Antibody (PA5-96452) in WB
Western blot analysis of USP16 in various lysates. Samples were incubated with USP16 Polyclonal antibody (Product # PA5-96452) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-96452
产品规格
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human USP16.
Conjugate
Unconjugated
形式
Liquid
浓度
0.99 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2808254
Product Specific Information
Immunogen sequence: MGKKRTKGKT VPIDDSSETL EPVCRHIRKG LEQGNLKKAL VNVEWNICQD CKTDNKVKDK AEEETEEKPS VWLCLKCGHQ GCGRNSQEQH ALKHYLTPRS EPHCLVLSLD NWSVWCYVCD NEVQYCSSNQ LGQVVDYVRK QASITTPKPE KDNGNIELEN KKLEKESKNE QEREKKENMA KENPPMNSPC QITVKGLSNL GNTCFFNAVM QNLSQTPVLR ELLKEVKMSG TIVKIEPPDL ALTEPLEINL EPPGPLTLAM SQFLNEMQET KKGVVTPKEL; Positive Samples: COS-7, 293T, NIH/3T3, Mouse brain, Mouse heart, Mouse spleen, Rat brain; Cellular Location: Nucleus
Target Information
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme 16; deubiquitinating enzyme 16 {ECO:0000255|HAMAP-Rule:MF_03062}; Ubiquitin carboxyl-terminal hydrolase 16; ubiquitin carboxyl-terminal hydrolase 16 {ECO:0000255|HAMAP-Rule:MF_03062}; ubiquitin specific protease 16; Ubiquitin thioesterase 16; ubiquitin thioesterase 16 {ECO:0000255|HAMAP-Rule:MF_03062}; ubiquitin thiolesterase 16; Ubiquitin-processing protease UBP-M; ubiquitin-specific processing protease 16; Ubiquitin-specific-processing protease 16; ubiquitin-specific-processing protease 16 {ECO:0000255|HAMAP-Rule:MF_03062}; USP16
Gene Aliases: 1200004E02Rik; 2810483I07Rik; 6330514E22Rik; MSTP039; UBP-M; UBPM; USP16
UniProt ID: (Human) Q9Y5T5, (Rat) Q2KJ09, (Mouse) Q99LG0
Entrez Gene ID: (Human) 10600, (Rat) 288306, (Mouse) 74112
Molecular Function:
cysteine protease
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
ubiquitin-dependent protein catabolic process
cellular response to DNA damage stimulus
cell cycle
mitotic nuclear division
histone deubiquitination
monoubiquitinated histone H2A deubiquitination
positive regulation of transcription, DNA-templated
positive regulation of translational elongation
positive regulation of transcription from RNA polymerase II promoter
protein homotetramerization
cell division
regulation of cell cycle
histone H2A K63-linked deubiquitination
biological_process
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of proteasomal protein catabolic process
regulation of transcription, DNA-templated
proteolysis
chromatin modification
transcription coactivator activity
cysteine-type endopeptidase activity
thiol-dependent ubiquitin-specific protease activity
zinc ion binding
histone binding
ubiquitin binding
molecular_function
protein binding
peptidase activity
cysteine-type peptidase activity
hydrolase activity
thiol-dependent ubiquitinyl hydrolase activity
metal ion binding
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