Invitrogen

Ubiquitin (linkage specific K27) Recombinant Rabbit Monoclonal Antibody (JE75-30)

问题与答案

问题与答案

问题与答案

抗体检测数据 (6)

Ubiquitin (linkage specific K27) Antibody in Immunocytochemistry (ICC/IF)

Ubiquitin (linkage specific K27) Antibody in Immunocytochemistry (ICC/IF)

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Ubiquitin (linkage specific K27) Antibody (MA570070) in ICC/IF

Immunocytochemistry analysis of Ubiquitin (linkage specific K27) in Jurkat cells. Samples were incubated with Ubiquitin (linkage specific K27) monoclonal antibody (Product # MA570070) using a dilution of 1:1,000 in 1% BSA in PBST overnight at 4°C followed by Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabili... View More

Ubiquitin (linkage specific K27) Antibody in Immunocytochemistry (ICC/IF)

Ubiquitin (linkage specific K27) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Ubiquitin (linkage specific K27) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Ubiquitin (linkage specific K27) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Ubiquitin (linkage specific K27) Antibody in Flow Cytometry (Flow)

Ubiquitin (linkage specific K27) Antibody in Dot Blot (DB)

Product Image Gallery

Ubiquitin (linkage specific K27) Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of Ubiquitin (linkage specific K27) in Jurkat cells. Samples were incubated with Ubiquitin (linkage specific K27) monoclonal antibody (Product # MA570070) using a dilution of 1:1,000 in 1% BSA in PBST overnight at 4°C followed by Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1:100 dilution overnight at 4°C. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1:1,000 dilution.

Ubiquitin (linkage specific K27) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Ubiquitin (linkage specific K27) in paraffin-embedded rat spleen tissue. Samples were incubated with Ubiquitin (linkage specific K27) monoclonal antibody (Product # MA570070) using a dilution of 1:1,000 for 1 hour at room temperature. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Ubiquitin (linkage specific K27) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Ubiquitin (linkage specific K27) in paraffin-embedded mouse spleen tissue. Samples were incubated with Ubiquitin (linkage specific K27) monoclonal antibody (Product # MA570070) using a dilution of 1:200 for 1 hour at room temperature. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Ubiquitin (linkage specific K27) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Ubiquitin (linkage specific K27) in paraffin-embedded human spleen tissue. Samples were incubated with Ubiquitin (linkage specific K27) monoclonal antibody (Product # MA570070) using a dilution of 1:200 for 1 hour at room temperature. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Ubiquitin (linkage specific K27) Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of Ubiquitin (linkage specific K27) in Jurkat cells. Samples were stained with Ubiquitin (linkage specific K27) monoclonal antibody (Product # MA570070) using a dilution of 1:1,000 (red) at 4°C for an hour followed by iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at a dilution of 1:1,000 for 30 minutes at 4°C. Cells were fixed and permeabilized. Rabbit IgG Isotype Control (green). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Ubiquitin (linkage specific K27) Antibody in Dot Blot (DB) — Dot blot analysis of Ubiquitin (linkage specific K27) in different proteins. Samples were incubated with Ubiquitin (linkage specific K27) monoclonal antibody (Product # MA570070) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG - HRP Secondary Antibody at a dilution of 1:50,000 for 1 hour at room temperature. Lane 1: Unmodified Ubiquitin peptide (negative); Lane 2: K27-linkage Specific Ubiquitin peptide (positive); Lane 3: K6-linkage Specific Ubiquitin peptide (negative); Lane 4: K11-linkage Specific Ubiquitin peptide (negative); Lane 5: K29-linkage Specific Ubiquitin peptide (negative); Lane 6: K33-linkage Specific Ubiquitin peptide (negative); Lane 7: K48-linkage Specific Ubiquitin peptide (negative); Lane 8: K63-linkage Specific Ubiquitin peptide (negative). Proteins loading: 100 ng, 25 ng, 5 ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 3 minutes; ECL.

Immunogen image

Ubiquitin (linkage specific K27) Recombinant Rabbit Monoclonal Antibody (JE75-30)

Immunogen Image Ubiquitin (linkage specific K27) Recombinant Rabbit Monoclonal Antibody (JE75-30)

产品信息

MA570070

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

-

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

-

免疫细胞化学 (ICC/IF)

1:1,000

-

流式细胞分析 (Flow)

1:1,000

-

点杂交 (DB)

1:1,000

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JE75-30

抗原

Synthetic peptide within human ubiquitin aa 20-34.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 0.05% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Positive control: Jurkat, human spleen tissue, mouse spleen tissue, rat spleen tissue.

Subcellular location: Cell Membrane, Cytoplasmic and Nuclear.

靶标信息

Ubiquitin is a small regulatory protein predominantly known for its role in protein degradation via the ubiquitin-proteasome pathway. Post-translational modifications of ubiquitin, such as polyubiquitination, involve linking ubiquitin molecules through one of its seven lysine residues (K6, K11, K27, K29, K33, K48, and K63). The K27-linked ubiquitin chains are of particular interest due to their involvement in non-proteolytic functions, including DNA damage response, transcriptional regulation, and immune response. K27-linked polyubiquitination can signal for the recruitment of specific binding proteins that recognize this distinct linkage, influencing various cellular processes. This specific linkage is structurally distinct from other types of ubiquitin chains, forming a compact and rigid conformation that determines its unique binding properties and functional outcomes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: epididymis secretory protein Li 50; polyubiquitin B; Polyubiquitin-B; ubiquitin C

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基因别名: AL033289; HEL-S-50; Rps27a; Uba52; UBB; Ubb2; UBC

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UniProt ID: (Human) P0CG47, (Mouse) P0CG49, (Rat) P0CG51

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Entrez Gene ID: (Human) 7314, (Mouse) 22187, (Rat) 192255

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protein binding

male meiosis I female meiosis I male gonad development female gonad development ubiquitin homeostasis hypothalamus gonadotrophin-releasing hormone neuron development mitochondrion transport along microtubule neuron projection morphogenesis regulation of mitochondrial membrane potential adipose tissue development fat pad development regulation of proteasomal protein catabolic process seminiferous tubule development energy homeostasis regulation of neuron death positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator G2/M transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter MAPK cascade activation of MAPK activity protein polyubiquitination nucleotide-excision repair, DNA damage recognition nucleotide-excision repair, DNA duplex unwinding stimulatory C-type lectin receptor signaling pathway MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway glycogen biosynthetic process transcription-coupled nucleotide-excision repair nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest transforming growth factor beta receptor signaling pathway Notch signaling pathway I-kappaB kinase/NF-kappaB signaling JNK cascade fibroblast growth factor receptor signaling pathway regulation of tumor necrosis factor-mediated signaling pathway regulation of necrotic cell death Wnt signaling pathway endosomal transport macroautophagy viral life cycle virion assembly translesion synthesis negative regulation of transforming growth factor beta receptor signaling pathway anaphase-promoting complex-dependent catabolic process positive regulation of protein ubiquitination regulation of type I interferon production negative regulation of type I interferon production tumor necrosis factor-mediated signaling pathway nucleotide-excision repair, DNA incision ion transmembrane transport TRIF-dependent toll-like receptor signaling pathway interstrand cross-link repair NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway ERBB2 signaling pathway negative regulation of epidermal growth factor receptor signaling pathway error-prone translesion synthesis DNA damage response, detection of DNA damage protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling proteasome-mediated ubiquitin-dependent protein catabolic process regulation of mRNA stability cellular protein metabolic process innate immune response positive regulation of epidermal growth factor receptor signaling pathway positive regulation of transcription from RNA polymerase II promoter T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity stress-activated MAPK cascade negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Wnt signaling pathway, planar cell polarity pathway regulation of transcription from RNA polymerase II promoter in response to hypoxia nucleotide-binding oligomerization domain containing signaling pathway global genome nucleotide-excision repair error-free translesion synthesis intracellular transport of virus negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway regulation of signal transduction by p53 class mediator positive regulation of protein monoubiquitination