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Invitrogen
V5 Tag Monoclonal Antibody (SV5-Pk1), FITC
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V5 Tag Antibody (MA1-80281) in ICC/IF
Immunofluorescent analysis of V5 Tag was performed using 70% confluent V5-H3-6XHis transfected HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with V5 Tag Monoclonal Antibody (SV5-Pk1), FITC (Product # MA1-80281) at 1:100 dilution and Histone H3 Recombinant Polyclonal Antibody (Product # 711055) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled wit... View More
产品信息
MA1-80281
产品规格
种属反应
Tag
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
SV5-Pk1
抗原
Paramyxovirus SV5, Simian-Virus 5.
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
0.1 mg/mL
纯化类型
Protein A
保存液
PBS with 1% BSA
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA1-80281 recognizes the sequence IPNPLLGL, present on the P/V proteins of the paramyxovirus, SV5. This antibody has been used to detect recombinant proteins, including epitope-tagged transmembrane and secreted proteins. Successfully used tags include a 14-amino acid tag (gly lys pro ile pro asn pro leu leu gly leu asp ser thr) and a smaller 9-amino acid tag that has been used to inhibit the binding of the monoclonal antibody to its native protein.
靶标信息
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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