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Invitrogen
VCAM-1 Polyclonal Antibody, PeproTech®
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VCAM-1 Antibody (500-P300-1MG) in WB
Western Blot: To detect hVCAM-1 by Western Blot analysis VCAM-1 Polyclonal Antibody (Product # 500-P300-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hVCAM-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P300-1MG
产品规格
种属反应
Human
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
HEK293 cells-derived Recombinant Human VCAM-1
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
产品详细信息
AA Sequence of recombinant protein: FKIETTPESR YLAQIGDSVS LTCSTTGCES PFFSWRTQID SPLNGKVTNE GTTSTLTMNP VSFGNEHSYL CTATCESRKL EKGIQVEIYSF PKDPEIHLSG PLEAGKPITV KCSVADVYPF DRLEIDLLKG DHLMKSQEFL EDADRKSLET KSLEVTFTPV IEDIGKVLVC RAKLHIDEMD SVPTVRQAVK ELQVYISPKN TVISVNPSTK LQEGGSVTMT CSSEGLPAPE IFWSKKLDNG NLQHLSGNAT LTLIAMRMED SGIYVCEGVN LIGKNRKEVE LIVQEKPFTV EISPGPRIAA QIGDSVMLTC SVMGCESPSF SWRTQIDSPL SGKVRSEGTN STLTLSPVS FENEHSYLCT VTCGHKKLEK GIQVELYSFPR DPEIEMSGGLV NGSSVTVSCK VPSVYPLDRLE IELLKGETILE NIEFLEDTDM KSLENKSLEMT FIPTIEDTGKA LVCQAKLHID DMEFEPKQRQ STQTLYVNVA PRDTTVLVSP SSILEEGSSV NMTCLSQGFP APKILWSRQL PNGELQPLSE NATLTLISTK MEDSGVYLCE GINQAGRSRK EVELIIQVTP KDIKLTAFPS ESVKEGDTVI ISCTCGNVPE TWIILKKKAE TGDTVLKSID GAYTIRKAQL KDAGVYECES KNKVGSQLRS LTLDVQGREN NKDYFSP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human VCAM-1. Anti-Human VCAM-1-specific antibody was purified by affinity chromatography employing an immobilized Human VCAM-1 matrix.
Sandwich ELISA: To detect Human VCAM-1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human VCAM-1 (500-P300Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human VCAM-1.
Western Blot: To detect hVCAM-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hVCAM-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD106; CD106 antigen; DKFZp779G2333; INCAM-100; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; V-CAM 1; Vascular cell adhesion protein 1
基因别名: CD106; INCAM-100; VCAM1
UniProt ID: (Human) P19320
Entrez Gene ID: (Human) 7412
response to hypoxia
acute inflammatory response
chronic inflammatory response
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
cell-matrix adhesion
aging
response to nutrient
amine metabolic process
response to zinc ion
response to ionizing radiation
viral process
membrane to membrane docking
B cell differentiation
extracellular matrix organization
response to lipopolysaccharide
response to nicotine
calcium-mediated signaling using intracellular calcium source
cellular response to vascular endothelial growth factor stimulus
positive regulation of T cell proliferation
response to ethanol
regulation of immune response
leukocyte tethering or rolling
oxidation-reduction process
cell chemotaxis
interferon-gamma-mediated signaling pathway
cellular response to tumor necrosis factor
