Invitrogen

VCAM-1 Monoclonal Antibody (1.G11B1)

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VCAM-1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of VCAM-1 was performed using 70% confluent log phase HAEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VCAM-1 Mouse monoclonal Antibody (Product # MA5-16429) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2500 for 1 hour at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

VCAM-1 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of KM-H2 cells stained with Mouse anti Human CD106

VCAM-1 Antibody in Western Blot, Neutralization, Inhibition Assays (WB, Neu, IA) — Figure 2 The enhanced expression of ICAM-1 or VCAM-1 is not responsible for the PT-triggered tumor cell adhesion (A/E) Confluent HUVECs were treated with PT (10, 30, 100 nM) or TNFalpha (10 ng/ml) for 12 h. The mRNA expression of ICAM-1 (A) or VCAM-1 (E) was analyzed by qPCR experiments. (B/F) Confluent HUVECs were treated with PT (10, 30, 100 nM) or TNFalpha (10 ng/ml) for 24 h. The total protein expression of ICAM-1 (B) or VCAM-1 (F) was determined by western blot analysis. (C/G) Confluent HUVECs were treated with PT (30, 100 nM) or TNFalpha (10 ng/ml) for 24 h. The cell surface expression of ICAM-1 (C) or VCAM-1 (G) was analyzed by flow cytometry. (D/H) Confluent HUVECs were treated with 30 nM PT for 24 h. An ICAM-1 blocking antibody (1, 5, 10 mug/ml) (D) or a VCAM-1 blocking antibody (1, 5, 10 mug/ml) (H) was added for the last 30 min of PT treatment. Fluorescence-labeled MDA cells were added and were allowed to adhere for 10 min. The amount of adherent MDA cells was determined by fluorescence measurements. (A-H) Data are expressed as mean +- SEM. A-C and E-H: n=3, D: n=5. *p <= 0.05 versus negative control, n.s.: p > 0.05 versus PT alone. TNFalpha was used as positive control and was not included into the statistical analyses.

产品信息

MA5-16429

应用	建议稀释比	已发表文章
免疫印迹 (WB)	Assay-dependent	查看1篇已发表文章 1篇已发表文章
免疫组化（冰冻） (IHC (F))	Assay-dependent	-
免疫细胞化学 (ICC/IF)	5 µg/ml	-
流式细胞分析 (Flow)	1:10 -1:50	查看3篇已发表文章 3篇已发表文章
酶联免疫吸附实验 (ELISA)	Assay-dependent	-
免疫沉淀 (IP)	Assay-dependent	-
中和实验 (Neu)	-	查看3篇已发表文章 3篇已发表文章
功能检测 (FN)	Assay-dependent	-
抑制试剂 (IA)	-	查看1篇已发表文章 1篇已发表文章
其他PubMed (Misc)	-	查看1篇已发表文章 1篇已发表文章

产品规格
种属反应	Human, Non-human primate, Pig
已发表种属	Human
宿主/亚型	Mouse / IgG1
分类	Monoclonal
类型	Antibody
克隆号	1.G11B1
抗原	human endothelial cells
偶联物	Unconjugated FITC
形式	Liquid
浓度	1 mg/mL
纯化类型	Protein A
保存液	PBS
内含物	0.09% sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2537945

产品详细信息

Non-reducing conditions are recommended for Western blot applications.

靶标信息

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The pretubulysin-induced exposure of collagen is caused by endothelial cell retraction that results in an increased adhesion and decreased transmigration of tumor cells.

Oncotarget

Schwenk R,Stehning T,Bischoff I,Ullrich A,Kazmaier U,Fürst R

Published figure using VCAM-1 monoclonal antibody (Product # MA5-16429) in Inhibition Assays

Fri Sep 29 00:00:00 EDT 2017

The pretubulysin-induced exposure of collagen is caused by endothelial cell retraction that results in an increased adhesion and decreased transmigration of tumor cells.

Oncotarget

Schwenk R,Stehning T,Bischoff I,Ullrich A,Kazmaier U,Fürst R

Published figure using VCAM-1 monoclonal antibody (Product # MA5-16429) in Inhibition Assays

Fri Sep 29 00:00:00 EDT 2017

The pretubulysin-induced exposure of collagen is caused by endothelial cell retraction that results in an increased adhesion and decreased transmigration of tumor cells.

Oncotarget

Schwenk R,Stehning T,Bischoff I,Ullrich A,Kazmaier U,Fürst R

Published figure using VCAM-1 monoclonal antibody (Product # MA5-16429) in Inhibition Assays

Fri Sep 29 00:00:00 EDT 2017

Adhesion of human hematopoietic progenitor cells to mesenchymal stromal cells involves CD44.

Cells, tissues, organs

Wagner W,Wein F,Roderburg C,Saffrich R,Diehlmann A,Eckstein V,Ho AD

MA5-16429 was used in blocking or activating experiment to test whether CD44 aids in adhesion of human hematopoietic progenitor cells to mesenchymal stromal cells

Fri Sep 19 00:00:00 EDT 2008

Effects of vascular endothelial growth factor on the lymphocyte-endothelium interactions: identification of caveolin-1 and nitric oxide as control points of endothelial cell anergy.

Journal of immunology (Baltimore, Md. : 1950)

Bouzin C,Brouet A,De Vriese J,Dewever J,Feron O

MA5-16429 was used in blocking or activating experiment to clarify mechanisms that regulate tumor endothelial cells anergy

Thu Feb 01 00:00:00 EST 2007

Immunological and ultrastructural characterization of endothelial cell cultures differentiated from human cord blood derived endothelial progenitor cells.

Histochemistry and cell biology

Neumüller J,Neumüller-Guber SE,Lipovac M,Mosgoeller W,Vetterlein M,Pavelka M,Huber J

MA5-16429 was used in flow cytometry to assess the ability of endothelial progenitor cells to differentiate into early mature endothelial cells

Fri Dec 01 00:00:00 EST 2006

Effect of maternal anti-HPA-1a antibodies and polyclonal IVIG on the activation status of vascular endothelial cells.

Clinical and experimental immunology

Radder CM,Beekhuizen H,Kanhai HH,Brand A

MA5-16429 was used in flow cytometry to test if anti-HPA-1a antibodies and intravenous immunoglobulin affect vascular endothelial cells in order to identify susceptibility for intracranial hemorrhage

Thu Jul 01 00:00:00 EDT 2004

Irradiation of mechanically-injured human arterial endothelial cells leads to increased gene expression and secretion of inflammatory and growth promoting cytokines.

Atherosclerosis

Wondergem J,Wedekind LE,Bart CI,Chin A,van der Laarse A,Beekhuizen H

MA5-16429 was used in flow cytometry to assess the effect of irradiation on re-endothelialisation of circular denuded tracks made in post-confluent cultures of arterial endothelial cells and cellular factors that contribute to this process

Thu Jul 01 00:00:00 EDT 2004

Human pulmonary valve endothelial cells express functional adhesion molecules for leukocytes.

The Journal of heart valve disease

Dvorin EL,Jacobson J,Roth SJ,Bischoff J

MA5-16429 was used in flow cytometry to explore how the cardiac valve endothelium is not damaged after transplantation.

Mon Sep 01 00:00:00 EDT 2003

生物信息学

蛋白别名: CD106; CD106 antigen; DKFZp779G2333; INCAM-100; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; V-CAM 1; Vascular cell adhesion protein 1

基因别名: CD106; INCAM-100; VCAM1

UniProt ID: (Human) P19320

Entrez Gene ID: (Human) 7412, (Pig) 396925

功能

response to hypoxia acute inflammatory response chronic inflammatory response cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion aging response to nutrient amine metabolic process response to zinc ion response to ionizing radiation viral process membrane to membrane docking B cell differentiation extracellular matrix organization response to lipopolysaccharide response to nicotine calcium-mediated signaling using intracellular calcium source cellular response to vascular endothelial growth factor stimulus positive regulation of T cell proliferation response to ethanol regulation of immune response leukocyte tethering or rolling oxidation-reduction process cell chemotaxis interferon-gamma-mediated signaling pathway cellular response to tumor necrosis factor

参与通路

integrin binding primary amine oxidase activity cell adhesion molecule binding

VCAM-1 Monoclonal Antibody (1.G11B1)

产品信息

MA5-16429

免疫印迹 (WB)

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

酶联免疫吸附实验 (ELISA)

免疫沉淀 (IP)

中和实验 (Neu)

功能检测 (FN)

抑制试剂 (IA)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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