Invitrogen

VCAM-1 Monoclonal Antibody (1.G11B1)

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VCAM-1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of VCAM-1 was performed using 70% confluent log phase HAEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VCAM-1 Mouse monoclonal Antibody (Product # MA5-16429) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2500 for 1 hour at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

VCAM-1 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of KM-H2 cells stained with Mouse anti Human CD106

VCAM-1 Antibody in Western Blot, Neutralization, Inhibition Assays (WB, Neu, IA) — Figure 2 The enhanced expression of ICAM-1 or VCAM-1 is not responsible for the PT-triggered tumor cell adhesion (A/E) Confluent HUVECs were treated with PT (10, 30, 100 nM) or TNFalpha (10 ng/ml) for 12 h. The mRNA expression of ICAM-1 (A) or VCAM-1 (E) was analyzed by qPCR experiments. (B/F) Confluent HUVECs were treated with PT (10, 30, 100 nM) or TNFalpha (10 ng/ml) for 24 h. The total protein expression of ICAM-1 (B) or VCAM-1 (F) was determined by western blot analysis. (C/G) Confluent HUVECs were treated with PT (30, 100 nM) or TNFalpha (10 ng/ml) for 24 h. The cell surface expression of ICAM-1 (C) or VCAM-1 (G) was analyzed by flow cytometry. (D/H) Confluent HUVECs were treated with 30 nM PT for 24 h. An ICAM-1 blocking antibody (1, 5, 10 mug/ml) (D) or a VCAM-1 blocking antibody (1, 5, 10 mug/ml) (H) was added for the last 30 min of PT treatment. Fluorescence-labeled MDA cells were added and were allowed to adhere for 10 min. The amount of adherent MDA cells was determined by fluorescence measurements. (A-H) Data are expressed as mean +- SEM. A-C and E-H: n=3, D: n=5. *p <= 0.05 versus negative control, n.s.: p > 0.05 versus PT alone. TNFalpha was used as positive control and was not included into the statistical analyses.

产品细节

Applications

Tested Dilution

Publications

免疫印迹 (WB)

Assay-dependent

View 1 publication 1 publication

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

5 µg/ml

流式细胞分析 (Flow)

1:10 -1:50

View 3 publications 3 publications

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

中和实验 (Neu)

View 3 publications 3 publications

功能检测 (FN)

Assay-dependent

抑制试剂 (IA)

View 1 publication 1 publication

其他PubMed (Misc)

View 1 publication 1 publication

产品规格

种属反应

Human, Non-human primate, Pig

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

1.G11B1

抗原

human endothelial cells

偶联物

Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2537945

产品详细信息

Non-reducing conditions are recommended for Western blot applications.

靶标信息

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: CD106; CD106 antigen; DKFZp779G2333; INCAM-100; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; V-CAM 1; Vascular cell adhesion protein 1

基因别名: CD106; INCAM-100; VCAM1

UniProt ID: (Human) P19320

Entrez Gene ID: (Human) 7412, (Pig) 396925

功能